Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,137 results for: biotin

Clear keywords
Showing: 201 - 250 of 1137 results

  3. Dead Streptavidin-SpyCatcher

    Plasmid
    #59547
    Purpose
    pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead-dloop-Streptavidin-SpyCatcher
    Tags
    N-terminally shortened SpyCatcher
    Expression
    Bacterial
    Mutation
    PolyAsp in L3/4 loop for ion-exchange
    Available Since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. TIRAP-TAP_IRES_GFP

    Plasmid
    #52740
    Purpose
    Retroviral vector expresses hTIRAP-3xHA and a target site for the biotin ligase BirA upstream of IRES and GFP
    Depositor
    Jonathan Kagan
    Article
    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10
    Insert
    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    Use
    Retroviral
    Tags
    3x HA, 3x TEV, and BirA target site
    Expression
    Mammalian
    Available Since
    July 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79887
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Expression
    Bacterial
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79887-plasmid-map-sequence-id-402553

    Enlarge

  18. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79889
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Expression
    Bacterial
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79889-plasmid-map-sequence-id-150065

    Enlarge

  20. pIRESneo2 mycBioID-Tollip M240A,F241A

    Plasmid
    #178055
    Purpose
    myc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.
    Depositor
    David Tumbarello
    Article
    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:
    Insert
    Toll-Interacting Protein (TOLLIP Human)
    Tags
    mycBioID
    Expression
    Mammalian
    Mutation
    Changed Methionine 240 to Alanine and changed Phe…
    Promoter
    CMV
    Available Since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    178055-plasmid-map-sequence-id-350118

    Enlarge

  26. p663-UBC-ER-TurboID-V5_IDG-K

    Plasmid
    #214453
    Purpose
    Gateway destination clone of ER-TurboID (endoplasmic reticulum control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    ER-TurboID-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214453-plasmid-map-sequence-id-425197

    Enlarge

  27. p663-UBC-PM-TurboID-V5_IDG-K

    Plasmid
    #214455
    Purpose
    Gateway destination clone of PM-TurboID (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    PM-TurboID-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214455-plasmid-map-sequence-id-423213

    Enlarge

  28. p663-UBC-PM-miniTurbo-V5_IDG-K

    Plasmid
    #214454
    Purpose
    Gateway destination clone of PM-miniTurbo (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    PM-miniTurbo-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214454-plasmid-map-sequence-id-433841

    Enlarge

  29. p663-UBC-ER-miniTurbo-V5_IDG-K

    Plasmid
    #214452
    Purpose
    Gateway destination clone of ER-miniTurbo (endoplasmic reticulum control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    ER-miniTurbo-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214452-plasmid-map-sequence-id-423209

    Enlarge

  30. p663-UBC-MTS-miniTurbo-V5_IDG-K

    Plasmid
    #214456
    Purpose
    Gateway destination clone of MTS-miniTurbo (mitochondrial control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    MTS-miniTurbo-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214456-plasmid-map-sequence-id-423210

    Enlarge

  31. p663-UBC-MTS-TurboID-V5_IDG-K

    Plasmid
    #214457
    Purpose
    Gateway destination clone of MTS-TurboID (mitochondrial control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    MTS-TurboID-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214457-plasmid-map-sequence-id-423208

    Enlarge

  33. p663-UBC-miniTurbo-V5-PIP5K1A_IDG-K

    Plasmid
    #214462
    Purpose
    Gateway destination clone of PIP5K1A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-PIP5K1A (PIP5K1A Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214462-plasmid-map-sequence-id-425204

    Enlarge

  34. p667-UBC-STK17A-V5-miniTurbo_IDG-K

    Plasmid
    #214466
    Purpose
    Gateway destination clone of STK17A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    STK17A-V5-miniTurbo (STK17A Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214466-plasmid-map-sequence-id-425202

    Enlarge

  35. p663-UBC-miniTurbo-V5-RIOK1_IDG-K

    Plasmid
    #214464
    Purpose
    Gateway destination clone of RIOK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-RIOK1 (RIOK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214464-plasmid-map-sequence-id-423214

    Enlarge

  36. p663-UBC-miniTurbo-V5-PRPF4B_IDG-K

    Plasmid
    #214463
    Purpose
    Gateway destination clone of PRPF4B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-PRPF4B (PRPF4B Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214463-plasmid-map-sequence-id-425201

    Enlarge

  37. p663-UBC-miniTurbo-V5-STK19_IDG-K

    Plasmid
    #214465
    Purpose
    Gateway destination clone of STK19 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-STK19 (STK19 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214465-plasmid-map-sequence-id-423215

    Enlarge

  38. p663-UBC-miniTurbo-V5-CDK11A_IDG-K

    Plasmid
    #214461
    Purpose
    Gateway destination clone of CDK11A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDK11A (CDK11A Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214461-plasmid-map-sequence-id-423211

    Enlarge

  39. p663-UBC-miniTurbo-V5-CSNK1G2_IDG-K

    Plasmid
    #214459
    Purpose
    Gateway destination clone of CSNK1G2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CSNK1G2 (CSNK1G2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214459-plasmid-map-sequence-id-423212

    Enlarge

  40. p663-UBC-miniTurbo-V5-CSNK1G3_IDG-K

    Plasmid
    #214460
    Purpose
    Gateway destination clone of CSNK1G3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CSNK1G3 (CSNK1G3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214460-plasmid-map-sequence-id-425206

    Enlarge

  41. p663-UBC-miniTurbo-V5-CSNK1G1_IDG-K

    Plasmid
    #214458
    Purpose
    Gateway destination clone of CSNK1G1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CSNK1G1 (CSNK1G1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214458-plasmid-map-sequence-id-425205

    Enlarge

  42. CP3

    Plasmid
    #223335
    Purpose
    Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    Toc64-miniTurboID-EGFP-3HA
    Tags
    miniTurboID-EGFP-3HA
    Expression
    Plant
    Promoter
    CaMV35S
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    223335-plasmid-map-sequence-id-455588

    Enlarge

  43. CP2

    Plasmid
    #223334
    Purpose
    Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    Toc64-EGFP-TurboID-3HA
    Tags
    EGFP-TurboID-3HA
    Expression
    Plant
    Promoter
    CaMV35S
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    223334-plasmid-map-sequence-id-455592

    Enlarge

  44. CP1

    Plasmid
    #223333
    Purpose
    Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    Toc64-TurboID-EGFP-3HA
    Tags
    TurboID-EGFP-3HA
    Expression
    Plant
    Promoter
    CaMV35S
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    223333-plasmid-map-sequence-id-455591

    Enlarge

  45. CP4

    Plasmid
    #223336
    Purpose
    Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    Toc64-EGFP-miniTurboID-3HA
    Tags
    EGFP-miniTurboID-3HA
    Expression
    Plant
    Promoter
    CaMV35S
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    223336-plasmid-map-sequence-id-455590

    Enlarge

  46. phage - ubc - nls - 2xmcp - egfp - BirA*

    Plasmid
    #131132
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Article
    Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11
    Insert
    NLS-HA-2xMCP-EGFP-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available Since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131132-plasmid-map-sequence-id-255500

    Enlarge

  47. PX4

    Plasmid
    #223340
    Purpose
    Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    ABCD1-EGFP-miniTurboID-3HA (PXA1 Mustard Weed)
    Tags
    EGFP-miniTurboID-3HA
    Expression
    Plant
    Promoter
    CaMV35S
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    223340-plasmid-map-sequence-id-455821

    Enlarge

  48. PX3

    Plasmid
    #223339
    Purpose
    Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    ABCD1-miniTurboID-EGFP-3HA (PXA1 Mustard Weed)
    Tags
    miniTurboID-EGFP-3HA
    Expression
    Plant
    Promoter
    CaMV35S
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    223339-plasmid-map-sequence-id-455820

    Enlarge

  49. PX2

    Plasmid
    #223338
    Purpose
    Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    ABCD1-EGFP-TurboID-3HA (PXA1 Mustard Weed)
    Tags
    EGFP-TurboID-3HA
    Expression
    Plant
    Promoter
    CaMV35S
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    223338-plasmid-map-sequence-id-455587

    Enlarge

  50. PX1

    Plasmid
    #223337
    Purpose
    Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    ABCD1-TurboID-EGFP-3HA (PXA1 Mustard Weed)
    Tags
    TurboID-EGFP-3HA
    Expression
    Plant
    Promoter
    CaMV35S
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    223337-plasmid-map-sequence-id-455823

    Enlarge

Showing: 201 - 250 of 1137 results