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We narrowed to 4,942 results for: codon optimized

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Showing: 81 - 100 of 4942 results

  2. pTPGI_dCas9_VP64

    Plasmid
    #49013
    Purpose
    encodes yeast-optimized dCas9_VP64 synthetic transcription factor
    Depositor
    Timothy Lu
    Publication
    Farzadfard et al ACS Synth Biol. 2013 Sep 11.
    Insert
    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Yeast
    Promoter
    pTPGI (galactose+aTc inducible)
    Available since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    49013-plasmid-map-sequence-id-231494

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  3. pFGC-pcoCas9

    Plasmid
    #52256
    Purpose
    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA
    Depositor
    Jen Sheen
    Insert
    35SPPDK:pcoCas9:NOS cassette
    Use
    CRISPR; Plant expression
    Promoter
    constitutive 35SPPDK promoter
    Available since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52256-plasmid-map-sequence-id-116013

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  5. BPK2101

    Plasmid
    #65770
    Purpose
    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    Use
    CRISPR
    Tags
    NLS-3xFlag
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65770-plasmid-map-sequence-id-112650

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  6. pCW57.1-FLAG-SRSF3_Full.Length_Codon.Optimized-Blast

    Plasmid
    #171951
    Purpose
    All-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, full-length SRSF3
    Depositor
    Sujatha Jagannathan
    Publication
    Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:
    Insert
    serine and arginine rich splicing factor 3 (SRSF3 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    codon-optimized
    Promoter
    TRE
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171951-plasmid-map-sequence-id-338270

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  8. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid
    #185914
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185914-plasmid-map-sequence-id-367363

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  9. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid
    #139988
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139988-plasmid-map-sequence-id-275338

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  10. pCW57.1-FLAG-SRSF3_Truncated_Codon.Optimized-Blast

    Plasmid
    #171952
    Purpose
    All-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, truncated SRSF3
    Depositor
    Sujatha Jagannathan
    Publication
    Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:
    Insert
    serine and arginine rich splicing factor 3 (SRSF3 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    codon-optimized, truncated at amino acid 114 with…
    Promoter
    TRE
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171952-plasmid-map-sequence-id-338274

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  11. pCFJ1415

    Plasmid
    #84828
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 introns
    Depositor
    Andrew Fire
    Publication
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Expression
    Bacterial and Worm
    Promoter
    Peft-3
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84828-plasmid-map-sequence-id-163670

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  15. p3xFLAG SARS-CoV2 RdRp.co

    Plasmid
    #154008
    Purpose
    Expression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cells
    Depositor
    Patrick Moore
    Publication
    Meng et al J Med Virol. 2023 Jan;95(1):e28246. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)
    Tags
    3xFLAG tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 23, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154008-plasmid-map-sequence-id-300786

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  16. pCFJ1137

    Plasmid
    #84827
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns
    Depositor
    Andrew Fire
    Publication
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Expression
    Bacterial and Worm
    Promoter
    Peft-3
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84827-plasmid-map-sequence-id-163671

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  17. pCMV-Nopt

    Plasmid
    #231704
    Purpose
    Mammalian expression vector encoding a codon-optimized N gene from vesicular stomatitis virus, strain Indiana.
    Depositor
    Ron Geller
    Publication
    Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VSV N gene (codon-optimized ) (VSIVgp1 vesicular stomatitis virus)
    Use
    Vsv expression
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    CMV
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231704-plasmid-map-sequence-id-459826

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Showing: 81 - 100 of 4942 results