We narrowed to 4,052 results for: plasmid lenti crispr

1. LentiCRISPR v2-sgAMPK alpha1 clone3

   Plasmid

   #162121

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCrisprV2 sgRNA hCRY1 c-term

   Plasmid

   #179453

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human CRY1
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCrisprV2 sgRNA hPER2c-term #1

   Plasmid

   #179454

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCrisprV2 sgRNA hPER2c-term #2

   Plasmid

   #179455

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCrisprV2 sgRNA hPER2c-term #3

   Plasmid

   #179456

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LentiCRISPR v2-sgAMPK alpha2 clone1

   Plasmid

   #162122

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha2

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha2 (PRKAA2 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LentiCRISPR v2-sgAMPK alpha2 clone2

   Plasmid

   #162123

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha2

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha2 (PRKAA2 Human)
   

   Use

   Lentiviral

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LentiCRISPR v2-sgAMPK alpha2 clone3

   Plasmid

   #162124

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha2

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha2 (PRKAA2 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

   Plasmid

   #138147

   Purpose

   Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NLS

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

    Plasmid

    #154429

    Purpose

    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

    Depositor

    David Liu

    Article

    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

    Insert

    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    VRQR point mutations in SpCas9

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid

    #217798

    Purpose

    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR and Lentiviral

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    EF1a

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. BRD4 CRISPRi plasmid

    Plasmid

    #154890

    Purpose

    Human BRD4 CRISPRi gRNA

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

    Insert

    BRD4-targeting gRNA
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-sgRNA

    Plasmid

    #71409

    Purpose

    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI

    Depositor

    Eric Lander, David Sabatini

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti.Cas9.BFP.sgRNA.parental

    Plasmid

    #196715

    Purpose

    Sortable WT-SpCas9 expression and guide cassette (1-vector system).

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Non-targeting CRISPRi plasmid

    Plasmid

    #154891

    Purpose

    Human non-targeting CRISPRi gRNA

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Lalli et al Genome Res. 2020 Sep;30(9):1317-1331. do

    Insert

    Nontargeting gRNA
    

    Use

    CRISPR and Lentiviral

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti.Cas9.BFP.Blast

    Plasmid

    #196714

    Purpose

    WT SpCas9 with BSD-TagBFP for selection and sorting

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRDA52_Lenti.puro.U6_modified DR

    Plasmid

    #196723

    Purpose

    For cloning pairs of AsCas12a guides with two DRs that are as different as possible.

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-dCas9-VP64-BFP

    Plasmid

    #196711

    Purpose

    CRISPR-activation. dCas9-VP64 with TagBFP for sorting

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-VP64-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-L3US2-RFP-HDAC3

    Plasmid

    #83966

    Purpose

    Lentiviral CRISPR HDAC3 dual gRNA targeting vector

    Depositor

    Eleanor Chen

    Insert

    HDAC3 dual gRNAs (HDAC3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti_dCas9-2xAM

    Plasmid

    #92220

    Purpose

    Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Inserts

    Sp-dCas9-2xAM tag
    gRNA to be inserted into Bbs I sites
    

    Use

    CRISPR and Lentiviral

    Tags

    2xAM tag

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    CBh and U6

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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