We narrowed to 29,541 results for: REP

1. pAG001 His6-TwinStrep-SUMO-AapCas12b

   Plasmid

   #153162

   Purpose

   Bacterial expression plasmid for AapCas12b

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

   Insert

   AapCas12b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. HBV 1.3-mer P-null replicon

   Plasmid

   #65462

   Purpose

   containing 1.3 units of the P-null HBV genome (subtype ayw)

   Depositor

   Wang-Shick Ryu

   Article

   Wang et al J Virol. 2009 Jun;83(11):5815-24. doi: 1

   Insert

   1.3 units of the P-null HBV genome
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-mCherry-P2A-strepII-PPPCB

   Plasmid

   #67923

   Purpose

   Expresses mCherry and strepII-tag-PP2aBeta in mammalian cells

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

   Insert

   mCherry-P2A-StrepII-PP2aBeta (Ppp2cb Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-FLAG-2xSTREP-CDKN1B-Puro

   Plasmid

   #172611

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDKN1B (CDKN1B Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-HA

   Plasmid

   #162100

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to VSVg-StrepTagII-HA
   

   Use

   Lentiviral

   Tags

   VSVg-StrepTagII-HA

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCD5-PR8-TEV-sfGFP-twinStrep

   Plasmid

   #157933

   Purpose

   Expresses influenza A virus trimeric hemagglutinin fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   Nemanichvili et al J Mol Biol. 2019 Feb 15;431(4):842-856.

   Insert

   A/PuertoRico/8/34 hemagglutinin (HA Synthetic)
   

   Use

   Tags

   sfGFP & TwinStrep

   Expression

   Mammalian

   Mutation

   E190D

   Promoter

   CMV

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-Enh eGFP Reporter-muKC2-X1

   Plasmid

   #59200

   Purpose

   Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

   Depositor

   Benjamin Yu

   Insert

   krt1-ex1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Mu Hsp68 minimal promoter

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCAG-TWIN-STREP-FLAG-NPRL2

   Plasmid

   #164985

   Purpose

   NPRL2 expressing vector for mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   NPRL2 (NPRL2 Human)
   

   Use

   Tags

   2xSTREP FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

   Plasmid

   #207031

   Purpose

   Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. T7 promoter + filler DNA reporter plasmid

    Plasmid

    #156454

    Purpose

    A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Inserts

    NeoR/KanR
    TcR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    Em7 and tet

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xFlag-tdMCP-Erk2 (pErk reporter)

    Plasmid

    #226167

    Purpose

    Used to transiently express the pErk reporter in LABEL-seq MAPK activity assays.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    3xFlag-tdMCP-Erk2 (MAPK1 Human)
    

    Use

    Tags

    3xFlag-tdMCP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJC-repo1.9kb-TALE4-VP64-P10

    Plasmid

    #104612

    Purpose

    Expresses TALE4 under the control of a 1.9kb repo enhancer element

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    1.9kb repo enhancer element (repo Fly)
    

    Use

    TALEN

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-28-Strep-OPA1(MGD) (SB264)

    Plasmid

    #227613

    Purpose

    Inducible expression of Strep-tagged Opa1(MGD) in E.coli

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    OPA1 (OPA1 Human)
    

    Use

    Tags

    Twin-StrepTag

    Expression

    Bacterial

    Mutation

    contains aa 253-580 (MGD) and aa 938-960

    Promoter

    T7

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-HA-AU1

    Plasmid

    #162113

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to StrepTagII-HA-AU1
    

    Use

    Lentiviral

    Tags

    StrepTagII-HA-AU1

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-HA-FLAG

    Plasmid

    #162092

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to StrepTagII-HA-FLAG
    

    Use

    Lentiviral

    Tags

    StrepTagII-HA-FLAG

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFA6a-6xGLY-Strep-tagII-HIS3MX6

    Plasmid

    #20756

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFl Strep-sumo-TEV-hAgo2

    Plasmid

    #136235

    Purpose

    Expresses human Argonaute-2 in insect cells

    Depositor

    Leemor Joshua-Tor

    Article

    Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

    Insert

    human Argonaute-2 (AGO2 Human)
    

    Use

    Tags

    Strep Sumo

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. 4A3.PBAD-repL(WT).J23115-gfp

    Plasmid

    #197872

    Purpose

    Arabinose induced expression of wildtype repL gene, which triggers DNA replication in cis and in trans, weak level of constitutive GFP expression

    Depositor

    Baojun Wang

    Article

    Huan et al Front Microbiol. 2023 Feb 9;14:1095671.

    Inserts

    part kilA/repL
    gfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    contains only the 24 bp of 3'-end of kilA ge…

    Promoter

    Bba_J23115 and PBAD

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52379

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    OCT4

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPuro3.1(+)_Strep-Lrrk2(G2019S)-Myc

    Plasmid

    #161585

    Purpose

    expression of Myc-tagged mouse Lrrk2 containing Parkinson's associated G2019S mutation

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    G2019S

    Promoter

    CMV

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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