We narrowed to 30,488 results for: ica

1. pDEST-hPDIb′a′

   Plasmid

   #60556

   Purpose

   enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

   Depositor

   Han Choe

   Article

   Song et al Appl Biochem Biotechnol. 2013 May;170(1)

   Type

   Empty backbone

   Use

   Tags

   His6-hPDIb'a' tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Plas-gRNA-LEU2

   Plasmid

   #157656

   Purpose

   GFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequence

   Depositor

   Frank Albert

   Article

   Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

   Insert

   GFP-gRNA
   

   Use

   Insert storage (replicative in e. coli)

   Tags

   GFP-gRNA

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hsa-miR-100 plix4.03

   Plasmid

   #121128

   Purpose

   inducible expression in cells

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   hsa-miR-100 (MIR100 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TN-XXL pRSETB

   Plasmid

   #45796

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

   Insert

   TN-XXL
   

   Use

   Tags

   6xHIS, ECFP, and YFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pSopB2xHA

   Plasmid

   #112513

   Purpose

   PsopB driven sopB-2xHA and sigE

   Depositor

   Olivia Steele-Mortimer

   Article

   Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

   Insert

   sopB-2xHA/sigE operon
   

   Use

   Tags

   2xHA

   Expression

   Bacterial

   Mutation

   SopB C-terminal 2xHA

   Promoter

   PsopB

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDH_ss-SBP-EGFP

   Plasmid

   #172358

   Purpose

   Reporter to monitoring a streptavidin-binding peptide trafficking

   Depositor

   Guido Kroemer

   Article

   Zhao et al Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.

   Insert

   ER importing signal sequence
   

   Use

   Lentiviral

   Tags

   SBP-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mTFP1-VSVG-16

   Plasmid

   #55517

   Purpose

   Localization: Golgi/ER Transport, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   VSVG
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   deltaK VSVG ts045

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

8. Plas-CRISPR_reporter

   Plasmid

   #157658

   Purpose

   CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

   Depositor

   Frank Albert

   Article

   Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

   Insert

   dCAS9-VP64, mCherry, KanMX
   

   Use

   Insert storage (replicative in e. coli)

   Tags

   Expression

   Mutation

   N28D in mCherry- please see depositor comments be…

   Promoter

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGBW-m4046841

   Plasmid

   #145578

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 helicase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pFA6a-TRP1-PGAL1-GFP

    Plasmid

    #41615

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP(S65T), TRP1 reverse orientation, and yeast AD…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMBP_hBrg1 (J1 antibody)

    Plasmid

    #26109

    Depositor

    Jerry Crabtree

    Article

    Wang et al EMBO J. 1996 Oct 1. 15(19):5370-82.

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. sspa-GCaMP6s

    Plasmid

    #67559

    Purpose

    short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6s
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pRSET-triple mutation-pa-GCaMP6f

    Plasmid

    #67570

    Purpose

    bacterial expression of a photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    pa-GCaMP6f
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial

    Mutation

    V115H, L64F, T65S

    Promoter

    T7

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. mTFP1-Lysosomes-20

    Plasmid

    #55495

    Purpose

    Localization: Lysosome Membrane, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    LAMP1
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pSB74

    Plasmid

    #19591

    Depositor

    Jay Keasling

    Article

    Bang et al Appl Environ Microbiol. 2000 Sep . 66(9)

    Insert

    thiosulfate reductase (phsB S. enterica serovar Typhimurium LT2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-hB50

    Plasmid

    #18967

    Depositor

    Mariano Viapiano

    Article

    Hu et al J Biol Chem. 2008 Jul 7. ():.

    Insert

    Brevican N-terminus (BCAN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Human brevican N-terminus aa 1-400

    Promoter

    Available since

    Dec. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

17. mTFP1-FAK-5

    Plasmid

    #55484

    Purpose

    Localization: Focal Adhesion Kinase, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    FAK
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

18. mTFP1-Paxillin-22

    Plasmid

    #55504

    Purpose

    Localization: Focal Adhesions, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Paxillin
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pGBW-m4046766

    Plasmid

    #145612

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. mTFP1-LC-Myosin-N-7

    Plasmid

    #55494

    Purpose

    Localization: Light Chain Myosin, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    LC-Myosin
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. vector plix 4.03

    Plasmid

    #121130

    Purpose

    inducible expression in cells

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    empty cassete for contol of miR expression
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pADH118-2

    Plasmid

    #90984

    Purpose

    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. albicans LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX28

    Plasmid

    #46850

    Purpose

    TOP RFP op. PGK-puro indicator plasmid to assay TOP IMCE efficiency

    Depositor

    Monica Hollstein

    Article

    Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

    Insert

    attB, RFP and PGK-puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGBW-m4046813

    Plasmid

    #145619

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. Tol2-lyzC-microRNA 146a-Dendra2

    Plasmid

    #97113

    Purpose

    overexpression of microRNA and Dendra2 in Zebrafish

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    microRNA 146a Dendra2
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mTFP1-Cx26-7

    Plasmid

    #55473

    Purpose

    Localization: Gap Junctions, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Cx26
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

27. Tol2-lyzC-microRNA 199-3-Dendra2

    Plasmid

    #97119

    Purpose

    overexpression of microRNA and Dendra2 in Zebrafish

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    microRNA 199 3 Dendra2
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mTFP1-Profilin-10

    Plasmid

    #55506

    Purpose

    Localization: Cytoskeleton, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Profilin
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pFA6a-TRP1-PGAL1-GST

    Plasmid

    #41612

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GST, TRP1 reverse orientation, and yeast ADH1 ter…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGBW-m4046898

    Plasmid

    #145707

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge