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Plasmid#221122PurposeFAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)DepositorInsertPMA1-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-RPA34-FAPoptim
Plasmid#221123PurposeFAP-tagged marker for the nucleus (FAP optimized for yeast expression)DepositorInsertRPA34-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-SEC7-FAPoptim
Plasmid#221124PurposeFAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)DepositorInsertSEC7-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-VPH1-FAPoptim
Plasmid#221125PurposeFAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)DepositorInsertVPH1-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-MFA-NFAPoptim
Plasmid#221090PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ERDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mNeonGreen in pRS315
Plasmid#221160PurposeFluorescent reporter for cytoplasmic aggregates under LEU2 selection markerDepositorInsertCarboxypeptidase Y (PRC1 Budding Yeast)
UseTagsmNeonGreenExpressionYeastMutationDeletion signal peptide, G255A mutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS415 GPDpro-RNQ1-CFP
Plasmid#224887PurposeLow copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).DepositorInsertRNQ1 (RNQ1 Budding Yeast)
UseTagsCFPExpressionYeastMutationPromoterGPDAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-DUET-C17orf53_1-87-Strep
Plasmid#211433Purposebacterial expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to a Strep-TagDepositorInsertC17orf53 (HROB Human)
UseTagsStrep-TagExpressionBacterialMutationDeletion of aa 88-646PromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-Duet-C17orf53-6His
Plasmid#211417Purposebacterial expression of human C17orf53 C-terminally fused to 6xHis-TagDepositorInsertC17orf53 (HROB Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-Duet-6His-C17orf53
Plasmid#211416Purposebacterial expression of human C17orf53 N-terminally fused to a 6xHis-TagDepositorInsertC17orf53 (HROB Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF- RPA4 (MSW#716)
Plasmid#208077PurposeExpression of human Replication Protein A 4 in E.coliDepositorInsertRPA4 (RPA4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET-WHaloCaMP-eNOSpep-EGFP
Plasmid#205306PurposeExpression of WHaloCaMP-eNOSpep-EGFP fusion in E. coliDepositorInsertWHaloCaMP-eNOSpep-EGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsDCP1a_539-582 (MCSII)_G
Plasmid#146407PurposeBacterial Expression of HsDCP1a_539-582DepositorInsertHsDCP1a_539-582 (DCP1A Human)
UseTagsExpressionBacterialMutationone silent mutation T1716G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsPatL1_517-767del664-673_J
Plasmid#146671PurposeBacterial Expression of HsPatL1_517-767-del664-673DepositorInsertHsPatL1_517-767-del664-673 (PATL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsPatL1_517-770-Mut1_J
Plasmid#146672PurposeBacterial Expression of HsPatL1_517-770-Mut1DepositorInsertHsPatL1_517-770-Mut1 (PATL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsPatL1_517-770-Mut2_J
Plasmid#146673PurposeBacterial Expression of HsPatL1_517-770-Mut2DepositorInsertHsPatL1_517-770-Mut2 (PATL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsPatL1_517-770-Mut3_J
Plasmid#146674PurposeBacterial Expression of HsPatL1_517-770-Mut3DepositorInsertHsPatL1_517-770-Mut3 (PATL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetP-HsPatL1_517-770-R591AR595AK625AK626A_J
Plasmid#146675PurposeBacterial Expression of HsPatL1_517-770-R591AR595AK625AK626ADepositorInsertHsPatL1_517-770-R591AR595AK625AK626A (PATL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-Uricase
Plasmid#182417PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-uricase fusionDepositorInsertVNp-mNeongreen-uricase
UseTagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-Erythropoietin
Plasmid#182423PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-Erythropoietin fusionDepositorInsertVNp-mNeongreen-eythropoietin (EPO Human)
UseTagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only