We narrowed to 4,313 results for: Pet2

1. pET28a-MH6-RspCas13d

   Plasmid

   #108305

   Purpose

   Expresses E. coli codon optimized RspCas13d in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

   Insert

   RspCas13d
   

   Use

   CRISPR

   Tags

   mH6 tag

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22b-6h-GST-TEVp

   Plasmid

   #172887

   Purpose

   Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Insert

   TEV protease
   

   Tags

   GST solubility tag and HHHHHH

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. FL MALT1 WT in pET29b-Strep

   Plasmid

   #48968

   Purpose

   Expresses Full length wild type MALT1 in e.coli

   Depositor

   Guy Salvesen

   Article

   Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

   Insert

   MALT1 isoform A (MALT1 Human)
   

   Tags

   His and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pET21a-TEX264_wt-His6

   Plasmid

   #220214

   Purpose

   Bacterial expression of wild type TEX264, C-terminal His6 tag

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-Streptavidin-K121R

   Plasmid

   #89880

   Purpose

   Streptavidin that has unimpaired biotin binding after dye labeling

   Depositor

   Mark Howarth

   Article

   Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

   Insert

   Streptavidin-K121R-Glutamate_Tag
   

   Tags

   6 glutamate tag (C terminal on insert)

   Expression

   Bacterial

   Mutation

   K121R

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-SnoopTagJr-MBP

   Plasmid

   #105628

   Purpose

   Plasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigase

   Depositor

   Mark Howarth

   Article

   Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

   Insert

   SnoopTagJr-MBP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-SBP-TEV

   Plasmid

   #36943

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   6xHistag-SBP-TEV

   Expression

   Bacterial

   Available Since

   June 19, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pET28b-His-hCE

   Plasmid

   #112710

   Purpose

   Expresses N-terminally His-tagged human CE/RNGTT in bacterial cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   RNGTT (RNGTT Human)
   

   Tags

   6xHis and Stop codons placed after RNGTT sequence…

   Expression

   Bacterial

   Mutation

   R594H

   Promoter

   T7

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21b-Casp1-His

   Plasmid

   #11809

   Depositor

   Guy Salvesen

   Insert

   Caspase-1 (CASP1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-Ec.coaBC (pCLK1210)

    Plasmid

    #50448

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

    Insert

    PPCS PPCDC
    

    Tags

    6XHIS tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET29b Tau Cys

    Plasmid

    #108867

    Purpose

    Expresses human Tau with a mutated cysteine

    Depositor

    Gabriele Kaminski Schierle

    Insert

    Tau (MAPT Human)
    

    Expression

    Bacterial

    Mutation

    I260C C291A C322A to allow maleimide dye labelling

    Promoter

    T7

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-MH6-RspWYL1

    Plasmid

    #108306

    Purpose

    Expresses E. coli codon optimized RspWYL1 in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Insert

    RspWYL1
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-SUMO-MjDim1

    Plasmid

    #189936

    Purpose

    Expression of Mjdim1, a methyltransferase used in m6A-SAC-seq.

    Depositor

    Chuan He

    Article

    Hu et al Nat Biotechnol. 2022 Mar 14. pii: 10.103

    Insert

    rsmA (MJ_RS00455 Methanocaldococcus jannaschii)
    

    Tags

    6x His tag, FLAG tag, SUMO tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. LOV-Turbo-His6_pET21a

    Plasmid

    #199668

    Purpose

    expresses LOV-Turbo in bacteria

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a_His-TEV-EiCsm6

    Plasmid

    #172487

    Purpose

    Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    Enterococcus italicus Csm6 (codon optimized)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7 promoter and lac operator

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET24a-AlkB deltaN11

    Plasmid

    #73622

    Purpose

    Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

    Depositor

    Catherine Drennan

    Article

    Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    AlkB (alkB E. coli)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    deletion of first 11 AA

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pET28_human-pro-IL18

    Plasmid

    #183390

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    pro-IL18 (IL18 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-SUMO-DogTag

    Plasmid

    #105629

    Purpose

    Plasmid for recombinant expression in bacteria of SUMO domain fused to DogTag, permitting covalent linkage to SnoopTagJr

    Depositor

    Mark Howarth

    Article

    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

    Insert

    SUMO-DogTag
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-His-hAos1

    Plasmid

    #53135

    Purpose

    E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    Aos1 (SAE1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET24a-LaM4-EGFP

    Plasmid

    #182641

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
    

    Tags

    BAP, EGFP, HA, His6, and T7

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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