We narrowed to 6,905 results for: crispr cas9 plasmids

1. pLX303-ABI1-dCas9

   Plasmid

   #154474

   Purpose

   Expresses ABI1 fused to the N terminus of dCas9

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ABI1 (ABI1 Mustard Weed)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Includes ABI1 aa 126-423, D143A

   Promoter

   CMV

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-HypaCas9-H2BC11_sgRNA

   Plasmid

   #183883

   Purpose

   pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 sgRNA spacer (H2BC11 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX459-HypaCas9-MYH10_sgRNA

   Plasmid

   #183887

   Purpose

   pX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   MYH10 sgRNA spacer (MYH10 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1

   Plasmid

   #121824

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9/LSD1 (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. IF405: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-p300core

   Plasmid

   #121825

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9/p300core (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. IA304: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-VPR

   Plasmid

   #121822

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9/VPR (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

   Plasmid

   #181745

   Purpose

   dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   human/zebrafish codon optimized SpCas9
   

   Use

   In vitro transcription; t7 promoter

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLL7 lenti-dcas9-3xflag-blast

   Plasmid

   #112133

   Purpose

   lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   dCas9(D10A, N863A)-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A mutants in Cas9

   Promoter

   EF1a

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21

   Plasmid

   #138295

   Purpose

   gRNA for CRISPR/Cas9 knockout of human TRIM21

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   gRNA TRIM21 (TRIM21 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLL8 lenti-D10Acas9-3xflag-blast

    Plasmid

    #112134

    Purpose

    lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    D10Acas9-3xflag-blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A mutant in Cas9

    Promoter

    EF1a

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZac2.1 SV40-CMV-SaCas9-3xNLS

    Plasmid

    #78601

    Purpose

    AAV vector containing SaCas9

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    SV40-CMV-SaCas9-3xNLS
    

    Use

    AAV and CRISPR

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    SV40, CMV promoters

    Available since

    Dec. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pspCas9-3exo-Tetra-com-CLCN5-sp-g1

    Plasmid

    #176236

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

    Plasmid

    #176237

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pspCas9-Exo-tetra-com-hCLCN5-sp-g1

    Plasmid

    #176238

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS3

    Plasmid

    #69229

    Purpose

    Expresses R1335K mutant SpCas9 fused to ZFP-TS3 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS3 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. px551-mSncg-0.27kb-HA-SpCas9

    Plasmid

    #153208

    Purpose

    Truncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cells

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Insert

    Cas9
    

    Use

    AAV and CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated mSncg (0.27kb)

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACT1:Cas9-GFP, U6:sgUPRT

    Plasmid

    #122853

    Purpose

    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Inserts

    Cas9-GFP
    U6-sgUPRT
    

    Use

    CRISPR; Cas9-gfp plasmid for genome editing of cr…

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Cryptosporidium parvum U6 and Cryptosporidium par…

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CMV-SpCas9D10A nickase

    Plasmid

    #216737

    Purpose

    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMVd1

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

    Plasmid

    #198750

    Purpose

    Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - SmallBiT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

    Plasmid

    #198751

    Purpose

    Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - LargeBiT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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