179,359 results
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Plasmid#110732PurposeMammalian Expression of BRAFV600E IRES H2B-mOrange2DepositorInsertBRAFV600E IRES H2B-mOrange2 (BRAF Human)
UseLentiviralExpressionMammalianMutationBRAF V600EAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo
Plasmid#102886PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmCherry-N1-EXO1b
Plasmid#111621PurposeExpression of human WT Exonuclease 1 (EXO1) tagged with mCherry in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertExonuclease 1b (EXO1 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40
Plasmid#100852PurposeAAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoterDepositorHas serviceAAV1InsertjRGECO1a
UseAAVExpressionMammalianPromoterCAGAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV-IRES_Tomato
Plasmid#107229PurposeTomato expressing plasmidDepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEJS613_pTetR-P2A-BFPnls/sgTelo
Plasmid#108649PurposeTelomere-targeting Spy sgRNA under U6 promoterDepositorInsertsTelomere-targeting sgRNA
TetR-P2A-BFP
UseCRISPR and LentiviralTagsNoExpressionMammalianPromoterU6 and hPGKAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP‐RNASEH2B
Plasmid#108697PurposeFor expression of N-terminally eGFP-tagged human RNASEH2B in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRNASEH2B (RNASEH2B Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAVTREGCaMP6f
Plasmid#97410PurposeAmplified expression of GCaMP6f in combination with TET driverDepositorInsertGCaMP6f
UseAAVExpressionMammalianPromoterTRE3Available sinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV_Thy1StTA
Plasmid#97411PurposeTET driver for amplified expression in cortical neuronsDepositorInserttTA
UseAAVExpressionMammalianPromotermouse thy1PSsAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0045-EF1a-PguCas13b-NES-HIV
Plasmid#103861PurposeExpresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasmDepositorInsertPguCas13b
UseCRISPR and LentiviralTags3xHA and HIV NESExpressionMammalianAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.Syn.Flex.GCaMP6s.WPRE.SV40
Plasmid#100845PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas serviceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-Cas9-162A
Plasmid#84918Purposefor in vitro transcription of Cas9 with polyA tail encodedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
TagsFlag, NLS, and polyA tailExpressionMammalianPromoterCAGAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT EGFP-miniAID
Plasmid#101714PurposeFor amplification of a EGFP-miniAID for N-terminal taggingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-miniAID
TagsEGFP-miniAIDExpressionMammalianAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40
Plasmid#101061PurposeAAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorHas serviceAAV1InsertCaMPARI2-F391W
UseAAVTagsFLAG-HA-myc and NES_hisPromoterhsyn (synapsin-1)Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-ihSyn1-DIO-tTA
Plasmid#99121PurposeAn AAV genome with Cre-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed expression of the tTADepositorHas serviceAAV1InsertDIO-tTA
UseAAVExpressionMammalianMutation*(see below)PromoterihSyn1Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
YIp204-PADH1-atTIR1-9myc
Plasmid#99532PurposeS. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)DepositorAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5
Plasmid#97044PurposeExpresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.DepositorInsertLigand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
UseLentiviralExpressionMammalianPromoterTet onAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
3xnls-mTurquoise2
Plasmid#98817PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xNLS
TagsmTurquoise2ExpressionMammalianPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-Nanoluc-ccdB
Plasmid#87070PurposeMammalian Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferaseDepositorTypeEmpty backboneTagsNanoluc luciferaseExpressionMammalianAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOD_A0101
Plasmid#90998PurposeModule A, Promoter: 35S, Gene: AtCas9, Terminator: HSPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAtCas9
UseCRISPRPromoter35SAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by