We narrowed to 21,628 results for: ESC;

1. pACUH-GFP1-10 mitochondrial matrix

   Plasmid

   #172063

   Purpose

   UAS vector to express GFP1-10 in the mitochondrial matrix

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   GFP1-10 mitochondrial matrix
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFX-mCherry-Sec61β

   Plasmid

   #174468

   Purpose

   Express mCherry with endoplasmic reticulum-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Venus with endoplasmic reticulum-targeting sequence
   

   Use

   Tags

   Sec61 subunit beta

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Str-KDEL_ManII-SBP-tagBFP

   Plasmid

   #65254

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and tagBFP

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. mRuby2-SiT-N-15

   Plasmid

   #55912

   Purpose

   Localization: Golgi TGN, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   SiT (ST6GAL1 Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   aa 1-45 of SiT

   Promoter

   CMV

   Available since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. E0030 (YFP)_CD

   Plasmid

   #66031

   Purpose

   MoClo Basic Part: CDS - Fluorescent protein. Yellow. [C:E0030:D]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   Fluorescent reporter - YFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFX-β-tubulin-SECFP

   Plasmid

   #175826

   Purpose

   Express SECFP with microtubule-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   SECFP with microtuble-targeting sequence
   

   Use

   Tags

   human tubulin beta 4B class IVb;TUBB4B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLBM0090

   Plasmid

   #172824

   Purpose

   Level 2 module containing mCHERRY-nVENUS HSP21-cVENUS

   Depositor

   Benjamin Field

   Article

   Velay et al Plant Methods. 2022 May 26;18(1):69. doi

   Insert

   [35S-P19][35S-CTPSSU-mHSP21-nVENUS][35S-CTPSSU-mHSP21-X3FLAGcVENUS][35S-SP_SV40-CFP]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p148

   Plasmid

   #62616

   Purpose

   ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR

   Depositor

   Mattheos Koffas

   Article

   Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

   Inserts

   flavaone 3B-hydroxylase
   dihydroflavonol 4-reductase
   leucoanthocyanidin reductase
   

   Use

   Synthetic Biology

   Tags

   GBD, PDZ, and SH3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Clover-Nup50-N-10

   Plasmid

   #56303

   Purpose

   Localization: Nuclear Pore, Excitation: 505, Emission: 515

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Nup50 (NUP50 Human)
   

   Use

   Tags

   Clover

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. bdSUMO-R9-GFP-HiBit

    Plasmid

    #170990

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-R9-muGFP-HiBiT
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS5

    Plasmid

    #55750

    Purpose

    Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

    Depositor

    Matthew Bennett

    Article

    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

    Insert

    KanR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Residues 59-264 of KanR

    Promoter

    T7-lac

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pgRNA_hMECP2_promoter_No4

    Plasmid

    #194899

    Purpose

    Lentiviral construct with mCherry and Puro cassettes to express sgRNA-4 targeting the promoter of human MECP2

    Depositor

    Shawn Liu

    Article

    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

    Insert

    sgRNA targeting human MECP2 promoter No.4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1X-Nup93-mut

    Plasmid

    #87335

    Purpose

    To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP93 (NUP93 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGDP3 rmtB

    Plasmid

    #112880

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    rmtB (RmtB Escherichia coli)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLBT0160

    Plasmid

    #172812

    Purpose

    Level 1 module containing P19_P1 F

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    p35S-5UTMV-CDS1_P19-3U+TERNOS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDx_mScarlet-I-SYFP2

    Plasmid

    #189761

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet-I-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I-SYFP2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV & Rha

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFX-β-tubulin-Sirius

    Plasmid

    #175825

    Purpose

    Express Sirius with microtubule-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with microtuble-targeting sequence
    

    Use

    Tags

    human tubulin beta 4B class IVb;TUBB4B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. NSSCP

    Plasmid

    #183190

    Purpose

    mRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEV

    Depositor

    Hsiao-Chun Huang

    Article

    Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

    Inserts

    mRFP-PopZ
    DivIVA-GFP(tevR-50)
    SpmX-TEV(C)
    TEV(N)-SpmX
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGBW-m4046801

    Plasmid

    #145741

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pFX-GalT-SECFP

    Plasmid

    #174470

    Purpose

    Express SECFP with golgi apparatus-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    SECFP with golgi apparatus-targeting sequence
    

    Use

    Tags

    β1,4-galactose transferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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