We narrowed to 30,903 results for: LIS;

1. pGL4.23-CD69 CaRE3 (minus strand)

   Plasmid

   #91845

   Purpose

   Luciferase reporter for CD69 enhancer (IGI-P0622)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   CD69 CaRE3 (minus strand) (CD69 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Missing 1 base from 13 base polyT tract beginning…

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SP6-VEE-OKS-iM

   Plasmid

   #58973

   Purpose

   RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Inserts

   Oct4 (POU5F1 Human)
   Klf4 (KLF4 Human)
   Sox2 (SOX2 Human)
   cMyc (MYC Human)
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Expression

   Mammalian

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SP6-VEE-OKS-iG

   Plasmid

   #58975

   Purpose

   RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Inserts

   Oct4 (POU5F1 Human)
   Klf4 (KLF4 Human)
   Sox2 (SOX2 Human)
   GLIS (GLIS1 Human)
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Expression

   Mammalian

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Ai139 (TIT2L-GFP-ICL-TPT) targeting vector

   Plasmid

   #114426

   Purpose

   Target a Cre-dependent GFP and a tdTomato-P2A-tTA2 expression cassette into the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   GFP, tdTomato, tTA2,
   

   Use

   Cre/Lox and Mouse Targeting

   Promoter

   TRE2, CAG

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Ai148 (TIT2L-GC6f-ICL-tTA2) targeting vector

   Plasmid

   #114428

   Purpose

   Target a Cre-dependent GCaMP6f expression and a tTA2 cassette into the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   GCaMP6f
   

   Use

   Cre/Lox and Mouse Targeting

   Promoter

   TRE2, CAG

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Ai163 (TIT2L-GC6s-ICL-TPT) targeting vector

   Plasmid

   #114430

   Purpose

   Target a Cre-dependent GCaMP6s cassette and a tdTomato-P2A-tTA2 cassette to the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   GCaMP6s, tdTomato, tTA2
   

   Use

   Cre/Lox and Mouse Targeting

   Promoter

   TRE2, CAG

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL4.23-IL2RA CaRE4 (plus strand)

   Plasmid

   #91850

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0345)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE4 (plus strand) (IL2RA Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Perfect match to reference sequence

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL4.23-IL2RA CaRE4 scramble (plus strand)

   Plasmid

   #91852

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0347)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE4 scramble (plus strand) (IL2RA Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Sequence scrambled from ch10:6094588-6094934

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pαH-S-GSAS-OMICRON

   Plasmid

   #180423

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

   Insert

   Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL4.23-IL2RA CaRE4 (minus strand)

    Plasmid

    #91849

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0626)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pαH-S-RRAR-OMICRON

    Plasmid

    #180424

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side intact

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike (S-RRAR-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pXPR_BRD051-sgMLH1-1

    Plasmid

    #125776

    Purpose

    constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    sgMLH1-1 (MLH1 Human)
    

    Use

    CRISPR

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pXPR_BRD051-sgMLH1-2

    Plasmid

    #125777

    Purpose

    constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    sgMLH1-2 (MLH1 Human)
    

    Use

    CRISPR

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ai140 (TIT2L-GFP-ICL-tTA2) targeting vector

    Plasmid

    #114427

    Purpose

    Target a Cre-dependent GFP and a tTA2 expression cassette into the mouse TIGRE locus

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    GFP, tTA2
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Mammalian

    Promoter

    PGK, CAG

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL4.23-IL2RA CaRE1 (plus strand)

    Plasmid

    #91837

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0614)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE1 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Ai133 (TITL-ssAPEX2tm) targeting vector

    Plasmid

    #114432

    Purpose

    Target a Cre-dependent electron microscopy tag ssAPEX2tm cassette into the mouse TIGRE locus

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    ssAPEX2tm
    

    Use

    Cre/Lox and Mouse Targeting

    Promoter

    TREtight

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pαH-S-GSAS-B.1.617.2.v1

    Plasmid

    #182575

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein Delta variant, furin side replaced by GSAS, 2 Proline at 986 and 987 plus T19R-G142D-del156/157-R158G-L452R-T478K-D614G-P681R-D950N

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike S-GSAS-B.1.617.2.v1 (T19R-G142D-Del156/157-R158G-L452R-T478K-D614G-P681R-D950N) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Ai167 (TIT2L-ChrimsonR-tdT-ICL-tTA2) targeting vector

    Plasmid

    #114431

    Purpose

    Target a Cre-dependent ChrimsonR-tdTomato cassette and a tTA2 cassette into the mouse TIGRE locus

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    ChrimsonR-tdTomato, tTA2
    

    Use

    Cre/Lox and Mouse Targeting

    Promoter

    TRE2, CAG

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Ai170 (TIT2L-ASAP2s-Kv-ICL-tTA2) targeting vector

    Plasmid

    #114435

    Purpose

    Target a Cre-dependent voltage sensor ASAP2s cassette into the mouse TIGRE locus

    Depositor

    Hongkui Zeng, Michael Lin

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    ASAP2s-Kv, tTA2
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Kv2.1 tag

    Promoter

    TRE2, CAG

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXPR_BRD003-sgMYC

    Plasmid

    #125770

    Purpose

    constitutive expression of a guide RNA targeting human MYC (CRISPR positive control)

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    sgMYC (MYC Human)
    

    Use

    CRISPR

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge