We narrowed to 18,496 results for: Met

1. RINS1(mut)

   Plasmid

   #107291

   Purpose

   Plasmid for the expression of the inactive mutant of RINS1 sensor

   Depositor

   Dmytro Yushchenko

   Article

   Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

   Insert

   RINS1(mut)
   

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   two Arg/Ser mutations at the beginning and in the…

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCX-SpiCee-NLS

   Plasmid

   #140900

   Purpose

   mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-NLS
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCHC042 (∆phaCAB)

   Plasmid

   #226208

   Purpose

   Conjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for phaCAB
   

   Use

   Synthetic Biology

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

   Plasmid

   #73404

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   At4CL
   PhCHS
   CmCHI
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. C413-E45: attB4-hr5-ie1p>-attB1r

   Plasmid

   #162930

   Purpose

   Gateway attB4/attB1r entry clone for transient insect expression using the ie1 promoter from AcMNPV

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   AcMNPV ie1 promoter with hr5 enhancer
   

   Use

   Synthetic Biology

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a(+)::NL3F10H

   Plasmid

   #141290

   Purpose

   NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   NLUC3F10H
   

   Use

   Luciferase

   Tags

   Flag (x3) and His (x10)

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMMB67EH-Spy

   Plasmid

   #90102

   Purpose

   IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.

   Depositor

   Samuel Miller

   Article

   Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

   Insert

   YcgR (ycgR )
   

   Use

   Broad-host range

   Expression

   Bacterial

   Promoter

   Ptac

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETSUMO2_Bacteroidetes-bGSDM-TEV

   Plasmid

   #222101

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Bacteroidetes-bGSDM-TEV ORF
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   TEV protease site replaces residues E242–G248

   Promoter

   T7

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5

   Plasmid

   #135455

   Purpose

   Bacterial expression of uTEV1Δ protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   MBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
   

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation improves stability, S153N mutation…

   Promoter

   tac promoter

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-FKBP-SYNDAPIN2

    Plasmid

    #221311

    Purpose

    Expression of SYNDAPIN2 (pLxIS) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    SYNDAPIN2 (pLxIS) (Pacsin2 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG-4xFKBP

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)

    Plasmid

    #117910

    Purpose

    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uM

    Depositor

    Kai Johnsson

    Article

    Yu et al Science. 2018 Sep 14;361(6407):1122-1126

    Insert

    SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    R44A on eDHFR

    Promoter

    T7

    Available Since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-21a-PE2-His

    Plasmid

    #201973

    Purpose

    Express His-tagged PE2 in bacteria

    Depositor

    Scot Wolfe

    Article

    Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

    Insert

    PE2-HIS
    

    Tags

    His and c-myc NLS

    Expression

    Bacterial

    Available Since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNBA001

    Plasmid

    #155323

    Purpose

    Binary vector for expression of PULSE-controlled EFR-GFP

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_(etr)8-(C120)5-PhCMVmin-EFR-GFP-T35S_RB
    

    Use

    Synthetic Biology; Eukaryotic expression

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNBA002

    Plasmid

    #155324

    Purpose

    Binary vector for expression of PULSE-controlled GBP-mCherry

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_(etr)8-(C120)5-PhCMVmin-GBP-mCherry-T35S_RB
    

    Use

    Synthetic Biology; Eukaryotic expression

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiX2-PURO-shLKB1-Hu

    Plasmid

    #61242

    Purpose

    Lentivirus expressing shRNA to human LKB1

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    LKB1 (STK11 Human)
    

    Use

    Lentiviral

    Available Since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-FLAG LIP

    Plasmid

    #15737

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    LIP (CEBPB Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSF-G1(7AW)RS

    Plasmid

    #207516

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-p3-PE(1152)-N-term

    Plasmid

    #187181

    Purpose

    p3-PE2-N-term(1152) in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Bock et al Sci Transl Med. 2022 Mar 16;14(636):eabl

    Insert

    PE2 from plasmid #132775
    

    Use

    AAV

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS MCK promoter

    Plasmid

    #12528

    Depositor

    Ronald Kahn

    Article

    Bruning et al Mol Cell. 1998 Nov . 2(5):559-69.

    Insert

    MCK promoter (Ckm Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. LOV-Turbo-V5_pRS415

    Plasmid

    #199667

    Purpose

    expresses LOV-Turbo in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Tags

    V5

    Expression

    Yeast

    Promoter

    Gal1,10

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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