We narrowed to 4,231 results for: Pet2

1. pET28C-mCherry-53BP1LCD

   Plasmid

   #204407

   Purpose

   E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   53BP1(1208-1977) (TP53BP1 Human)
   

   Use

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28sumo-hRig-I DCARDS

   Plasmid

   #210828

   Purpose

   protein expression in ecoli

   Depositor

   Anna Pyle

   Article

   Luo et al Cell. 2011 Oct 14;147(2):409-22. doi: 10

   Insert

   hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28b(+)_rPD-L1nb

   Plasmid

   #226715

   Purpose

   Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Recombinant PD-L1 nanobody
   

   Use

   Tags

   HA tag and Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21-HIS-Tol2

   Plasmid

   #112486

   Purpose

   functional recombinant Tol2 protein from E.coli

   Depositor

   Stephen Ekker

   Article

   Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

   Insert

   Tol2 Transposase (tpase Synthetic)
   

   Use

   Tags

   HIS/6x

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28_mouse-pro-IL18

   Plasmid

   #183389

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   pro-IL18 (Il18 Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET23b-Casp6-His

   Plasmid

   #11823

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 6 (CASP6 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-Ec.coaD (pESC106)

   Plasmid

   #50388

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

   Depositor

   Tadhg Begley, Erick Strauss

   Article

   Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

   Insert

   PPAT
   

   Use

   Tags

   6xHis-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-mH6-Cas12g1

   Plasmid

   #120879

   Purpose

   Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12g1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-Hook3 aa 1-552

   Plasmid

   #74614

   Purpose

   Expresses a truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-552 (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21a-Core Traptavidin

    Plasmid

    #26054

    Depositor

    Mark Howarth

    Article

    Chivers et al Nat Methods. 2010 Apr 11. ():.

    Insert

    Core Traptavidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Streptavidin with 2 amino acid mutations: S52G R5…

    Promoter

    Available since

    Aug. 30, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pET28a-HIV p24

    Plasmid

    #212195

    Purpose

    Bacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminus

    Depositor

    Richard C. Willson

    Insert

    Chain A, Capsid protein p24, HIV type 1
    

    Use

    Tags

    Polyhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET23b-Casp7-His

    Plasmid

    #11825

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 7 (CASP7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pET21b-Caspase-3(C163S)

    Plasmid

    #90088

    Purpose

    Expresses caspase 3 with C163S to prevent autocatalysis in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    C163S

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-Ec.coaE (pESC124)

    Plasmid

    #50390

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    DPCK
    

    Use

    Tags

    6XHIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21 MBPx-SpyCatcher

    Plasmid

    #72327

    Purpose

    Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET21 MBPx-SpyCatcher
    

    Use

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Mutation

    MBP contains A312V, I317V mutations and 172, 173,…

    Promoter

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21b Apaf1 1-591

    Plasmid

    #26642

    Depositor

    Guy Salvesen

    Article

    Pop et al Mol Cell. 2006 Apr 21. 22(2):269-75.

    Insert

    Apoptotic protease activating factor 1 (APAF1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acids 1-591

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pET28a-RBP-LOV2

    Plasmid

    #185792

    Purpose

    Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-LOV2
    

    Use

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a SnoopTag-MBP

    Plasmid

    #72323

    Purpose

    Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a SnoopTag-MBP
    

    Use

    Tags

    N-terminal His6 tag. MBP is fused at the C-termin…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21-Streptavidin-Glutamate_Tag

    Plasmid

    #46367

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

    Insert

    Streptavidin-Glutamate_Tag
    

    Use

    Tags

    6 glutamate tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

20. BARD1 26-327 pET28a

    Plasmid

    #12646

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    BRCA1 binding partner for a Ring domain E3 Ub-ligase (BARD1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge