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Plasmid#13571DepositorInsertFLIPglu F16A Delta11 Aphrodite
UseTagsAphrodite and AresExpressionBacterialMutationFluorophore-derived linker shortened. F16A mutati…PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pWP1_mFTCD
Plasmid#102317Purposeexpression of mouse cDNA of FTCD, refractory to the guides in plasmids 1,2DepositorInsertFtcd (Ftcd Mouse)
UseLentiviralTagsExpressionMutationPromoterEF1alphaAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSECB
Plasmid#106305PurposeExpress spCas9 with sgRNA, blast resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM6_Ptrc_BsMdh_BmHps_BmPhi
Plasmid#129104PurposeExpresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicusDepositorInsertBsMDH BmHPS BmPHI
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBabe-SIRT3
Plasmid#66167PurposeExpresses SIRT3 via retroviral methodDepositorInsertSIRT3 (Sirt3 Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationL235PPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti CRISPR V2 sgShmt2_2
Plasmid#106315PurposeExpress Cas9 and sgRNA targeting mShmt2DepositorInsertsgRNA targeting mShmt2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)
Plasmid#117910PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR44A on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-2 (C50 29 nM)
Plasmid#117909PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR98A on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLPC myc-mRAP1 I312R
Plasmid#73137PurposeExpression of myc-tagged mRAP1 with single point mutatoin I312RDepositorInsertRap1 (Terf2ip Mouse)
UseTagsmycExpressionMammalianMutationisoleucine changed to an arginine at amino acid 3…PromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-600uDelta13 Aphrodite
Plasmid#13570DepositorInsertFLIPglu F16A Delta13 Aphrodite
UseTagsAphrodite and ECFPExpressionBacterialMutationFluorophore-derived linker shortened. F16A mutati…PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
YFP(159-238) in pcDNAI/Amp
Plasmid#60889PurposeThis vector attaches YFP(159-238) to the N-terminus of a protein.DepositorInsertYFP(159-238)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-NDUFB4
Plasmid#139840PurposeLentiviral expression of human NDUFB4DepositorInsertNDUFB4 (NDUFB4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CB7.CI.PM20D2-flag.WPRE.rBG
Plasmid#132684PurposeAAV plasmid encoding mouse PM20D2 with C-terminal flag tagDepositorInsertPeptidase M20 domain containing 2 (Pm20d2 Mouse)
UseAAVTagsFlagExpressionMutationPromoterChicken b-actinAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA TAG233
Plasmid#105838PurposeExpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 233 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
UseTags6x his tagExpressionBacterialMutationTAG stop codon at site 233PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CRISPR sgSHMT2_2
Plasmid#106310PurposeExpress Cas9 and sgRNA targeting SHMT2DepositorInsertsgRNA targeting SHMT2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYG026
Plasmid#65328Purposereporter plasmids used in YeastFab work, in which promoters could be inserted just upstream YFP(YEVenus) to drive its expression, also contains an mCherry gene driven by TEF2 promoter.DepositorInsertccdB
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCRISPomyces-2BD
Plasmid#234429PurposePlasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning templateDepositorInsertModified dCas9(D10A, H840A) with polyaspartate linking
UseTagsExpressionBacterialMutationLinked polyaspartate using glycine-serine linkerPromoterAvailable sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80EN10-Pct3-(–)-Eldh1(cHis6)-noAccs(ElA2)
Plasmid#233880PurposeExpresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213DepositorInsert(–)-Eldh1 (Elen_0511 Eggerthella lenta)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80EN8-Pct3-(+)-Eddh(cHis6)-noAccs(ElA2)
Plasmid#233879PurposeExpresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213DepositorInsert(+)-Eddh (Elen_0501 Eggerthella lenta)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF26-Pct3-(+)-Eldh1(nHis6)(GpDSM19378)
Plasmid#233874PurposeExpresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(+)-Eldh1 (GPA_25810 Gordonibacter pamelaeae DSM 19378)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF14-Pct3-ElVadh(cHis6)-Accs(ElDSM2243)
Plasmid#233871PurposeExpresses His-tagged Eggerthella lenta DSM 2243 El Vadh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsertEl Vadh (Elen_0497 Eggerthella lenta)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF12-Pct3-(–)-Eldh1(cHis6)-Accs(ElA2)
Plasmid#233870PurposeExpresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(–)-Eldh1 (Elen_0511 Eggerthella lenta)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF10-Pct3-(+)-Eddh(cHis6)-Accs(ElA2)
Plasmid#233869PurposeExpresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(+)-Eddh (Elen_0501 Eggerthella lenta)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD70EN19-Pct5-GPA_25810/25800-(+)-Eldh1/2(GpDSM19378)
Plasmid#225305PurposeExpresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 and (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoterDepositorInsert(+)-Eldh1, (+)-Eldh2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF27-Pct3-(+)-Eldh2(nHis6)(GpDSM19378)
Plasmid#233875PurposeExpresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(+)-Eldh2 (GPA_25800 Gordonibacter pamelaeae DSM 19378)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHPHS926
Plasmid#228237PurposeBxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin NMD reporter with premature termination codon for integration into Bxb1 attP_GA landing padDepositorInsertsmCherry
Beta globin
UseTags3xFLAG, HA, and PuroRExpressionMammalianMutationPTC39PromoterTRE3GV and noneAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-TEF2p-LDH-CYC1t
Plasmid#216120PurposeYeast integrative vector for constitutive expression of LDH at the LEU2 locusDepositorInsertLactate dehydrogenase (LDH Bovine)
UseTagsExpressionYeastMutationPromoterTEF2 promoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TDH3p-LDH-CYC1t
Plasmid#216121PurposeYeast integrative vector for constitutive expression of LDH at the TRP1 locusDepositorInsertLactate dehydrogenase (LDH Bos taurus, Bovine)
UseTagsExpressionYeastMutationPromoterTDH3 promoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRRv2_sgTom
Plasmid#218346PurposeCRISPR KO plasmid for tdTomato in human cell linesDepositorInsertInserted sgRNA targetting tdTomato
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHC005 (∆RE)
Plasmid#226199PurposeConjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).DepositorInsertHomology arms for type I restriction-modification system restriction subunit H16_A0006
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBWB514
Plasmid#209326Purposep15A-tetR-tet-SapI-ME-RBS-mRFP-ME-U1-Barcode-U2-SapI-dbI terminator-oriT-Cm. Fragment expression vector with Golden Gate sites.DepositorInsertmRFP
UseTagsExpressionBacterialMutationPromotertetAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB108
Plasmid#222207PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with a C-terminal His tagDepositorInsertfghA
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-TASL
Plasmid#221265PurposeExpression of TASL in mammalian cells by retroviral transductionDepositorInsertTASL (Tasl Mouse)
UseRetroviralTags3xFLAGExpressionMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-YopE
Plasmid#221328PurposeDox-inducible expression of YopE in mammalian cells by retroviral transductionDepositorInsertYopE
UseRetroviralTagsFLAGExpressionMutationPromoterTRE3GAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-OPHN1
Plasmid#221310PurposeExpression of OPHN1 (pLxIS) in mammalian cells by retroviral transductionDepositorInsertOPHN1 (pLxIS) (Ophn1 Mouse)
UseRetroviralTagsFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-TRIF(Nterm)
Plasmid#221334PurposeOverexpression of TRIF Nterm domain in bacteriaDepositorInsertTRIF Nterm domain (Ticam1 Mouse)
UseTags6xHis-4xFKBPExpressionBacterialMutationPromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-ZC3H12B
Plasmid#221300PurposeExpression of ZC3H12B (pLxIS) in mammalian cells by retroviral transductionDepositorInsertZC3H12B (pLxIS)
UseRetroviralTagsFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-NMD3
Plasmid#221296PurposeExpression of NMD3 (pLxIS) in mammalian cells by retroviral transductionDepositorInsertNMD3 (pLxIS) (Nmd3 Mouse)
UseRetroviralTagsFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
plenti hSyn PGK1
Plasmid#220919PurposeNeuronal specific expression of PGK1DepositorInsertPGK1 (PGK1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV DJ-1 C106A
Plasmid#220914PurposeExpresses DJ-1 with the C106A mutationDepositorInsertDJ-1 (PARK7 Human)
UseTagsExpressionMammalianMutationC106APromoterCMVAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only