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We narrowed to 7,986 results for: 104

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Showing: 2501 - 2550 of 7986 results

  6. pD649-HAsp-VSIG2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156659
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    VSIG2 (VSIG2 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156659-plasmid-map-sequence-id-302427

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  13. pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry

    Plasmid
    #226104
    Purpose
    Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Insert
    COX8A (COX8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    encodes only for mitochondrial targeting sequence…
    Available Since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    226104-plasmid-map-sequence-id-448409

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  22. CD8a-AA347-363- RVEP-4A-GFP

    Plasmid
    #221597
    Purpose
    Expresses CD8a with of Arl13b ciliary targeting sequence (CTS) from amino acids 347-363 with RVEP4A mutation fused to the cytosolic tail and GFP-tagged
    Depositor
    Lei Lu
    Article
    Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi
    Insert
    Arl13B (ARL13B Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Amino acids 347-363 are inserted with mutation of…
    Promoter
    CMV promoter
    Available Since
    Dec. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    221597-plasmid-map-sequence-id-494682

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  23. CD8a-AA347-363-KRN-3A-GFP

    Plasmid
    #221598
    Purpose
    Expresses CD8a with of Arl13b ciliary targeting sequence (CTS) from amino acids 347-363 with KRN-3A mutation fused to the cytosolic tail and GFP-tagged
    Depositor
    Lei Lu
    Article
    Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi
    Insert
    Arl13B (ARL13B Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Amino acids 347-363 are inserted with mutation of…
    Promoter
    CMV promoter
    Available Since
    Dec. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    221598-plasmid-map-sequence-id-494676

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  24. pD649-HAsp-MPZL1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156511
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    MPZL1 (MPZL1 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Aug. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    156511-plasmid-map-sequence-id-368840

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  25. pT2iApowriter2(#336)

    Plasmid
    #184104
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transient transgenesis
    Tags
    internal tag: 5myc
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184104-plasmid-map-sequence-id-364017

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  26. pAd-M3cherry

    Plasmid
    #61041
    Purpose
    This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.
    Depositor
    Qiao Zhou
    Article
    Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.
    Inserts
    Ngn3 (Neurog3 Mouse)
    pdx1 (Pdx1 Mouse)
    Mafa (Mafa Mouse)
    monomeric cherry
    Use
    Adenoviral
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    61041-plasmid-map-sequence-id-456476

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  28. pCMV-T7-SpCas9(MRKQKC)-P2A-EGFP (RAS2838)

    Plasmid
    #223104
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKQKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRKQKC)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MRKQKC)=D1135M, S1136R, G1218K, E1219Q, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223104-plasmid-map-sequence-id-447670

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  30. TRSP-COMP-blac-flag-his

    Plasmid
    #110964
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    thrombospondin related sporozoite protein (TRSP) (PF3D7_0104000 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    110964-plasmid-map-sequence-id-224712

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  34. pTO_CHMP2B_LAP

    Plasmid
    #101848
    Purpose
    Tet-inducible expression of human CHMP2B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    CHMP2B (CHMP2B Human)
    Use
    Flp-frt recombination, tet-inducible expression
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    silent mutation in Thr 104; A to T
    Promoter
    CMV
    Available Since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101848-plasmid-map-sequence-id-200235

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  45. mEmerald-Hec1-N-12

    Plasmid
    #54119
    Depositor
    Michael Davidson
    Insert
    Hec1 (NDC80 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  46. pGEX-HE4

    Plasmid
    #18100
    Depositor
    Ronny Drapkin
    Insert
    Human Epididymis Protein 4 (WFDC2 Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    Feb. 27, 2009
    Availability
    Academic Institutions and Nonprofits only

  47. pcDNA3-HE4

    Plasmid
    #18101
    Depositor
    Ronny Drapkin
    Insert
    Human Epididymis Protein 4 (WFDC2 Human)
    Expression
    Mammalian
    Available Since
    June 11, 2008
    Availability
    Academic Institutions and Nonprofits only

  48. pAIP4_2H7W

    Plasmid
    #234220
    Purpose
    Heterologous protein expression of chagasin in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2H7W (Tc00.1047053506801.80 Trypanosoma cruzi)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234220-plasmid-map-sequence-id-464185

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  50. mCherry-Hec1-N-12

    Plasmid
    #55061
    Depositor
    Michael Davidson
    Insert
    Hec1 (NDC80 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 2501 - 2550 of 7986 results