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Showing: 2501 - 2550 of 13556 results

  13. hCas9_D10A

    Plasmid
    #41816
    Purpose
    Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering
    Depositor
    George Church
    Article
    Mali et al Science. 2013 Feb 15;339(6121):823-6. do
    Insert
    Cas9_D10A
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A nickase
    Promoter
    CMV
    Available since
    Jan. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    41816-plasmid-map-sequence-id-116715

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  14. pAB1

    Plasmid
    #62547
    Purpose
    broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.
    Depositor
    Michael Franklin
    Article
    Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47
    Insert
    eGFP
    Use
    Broad host range plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pTrc
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    62547-plasmid-map-sequence-id-314155

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  19. pKM3

    Plasmid
    #154168
    Purpose
    Empty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154168-plasmid-map-sequence-id-309416

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  20. p414-TEF1p-Cas9-CYC1t

    Plasmid
    #43802
    Purpose
    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter
    Depositor
    George Church
    Article
    Dicarlo et al Nucleic Acids Res
    Insert
    Human Optimized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1 promoter
    Available since
    March 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    43802-plasmid-map-sequence-id-333570

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  32. pHsp70-Cas9

    Plasmid
    #45945
    Purpose
    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.
    Depositor
    Jill Wildonger, Melissa Harrison, Kate O'Connor-Giles
    Article
    Gratz et al Genetics. 2013 May 24.
    Insert
    Codon optimized Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Expression
    Mutation
    Silent change from C to A at bp 834 of insert
    Promoter
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    45945-plasmid-map-sequence-id-116251

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  49. p415-GalL-Cas9-CYC1t

    Plasmid
    #43804
    Purpose
    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter
    Depositor
    George Church
    Article
    Dicarlo et al Nucleic Acids Res
    Insert
    Human Optimized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GalL
    Available since
    March 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    43804-plasmid-map-sequence-id-59154

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Showing: 2501 - 2550 of 13556 results