Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 24,285 results for: nes

Showing: 2501 - 2550 of 24285 results

  6. pRcCMV-STAT31F

    Plasmid
    #134725
    Purpose
    Expresses chimeric STAT3-STAT1 protein with human STAT3 amino acids 1-514 joined to human STAT1 at amino acid 509, with a FLAG epitope tag at the C-terminus. Based on addgene plasmid #8691 and #8707
    Depositor
    Curt Horvath, Jim Darnell
    Article
    Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.
    Insert
    STAT3-STAT1 Fusion (STAT3, STAT1 Human)
    Use
    Tags
    FLAG and human STAT3 aa 1-514 joined to human STA…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    134725-plasmid-map-sequence-id-272781

    Enlarge

  7. pRcCMV-STAT13F

    Plasmid
    #134723
    Purpose
    Expresses chimeric STAT1-STAT3 protein with human STAT1 amino acids 1-508 joined to human STAT3 at amino acid 515, with a FLAG epitope tag at the C-terminus. Based on addgene plasmids #8691 and #8707
    Depositor
    Curt Horvath, Jim Darnell
    Article
    Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.
    Insert
    STAT1-STAT3 fusion (STAT3, STAT1 Human)
    Use
    Tags
    FLAG and human STAT1 aa1-508 joined to human STAT…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    134723-plasmid-map-sequence-id-272780

    Enlarge

  9. pSLIK_hygro_3xflag_NRF2(RR)

    Plasmid
    #136535
    Purpose
    Lentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)
    Depositor
    Kevin Janes
    Article
    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13
    Insert
    NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    This plasmid is developed by mutating 3 base pair…
    Promoter
    Available since
    July 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    136535-plasmid-map-sequence-id-304676

    Enlarge

  11. RED

    Plasmid
    #139835
    Purpose
    Expresses RNase H1-EGFP- dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Article
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dCas9 protein contains two mutations: D10A and H8…
    Promoter
    tet-inducible CMV
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139835-plasmid-map-sequence-id-310615

    Enlarge

  12. dRED

    Plasmid
    #139836
    Purpose
    Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Article
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Use
    Tags
    Expression
    Mammalian
    Mutation
    RNase H1 protein contains D210N mutation. dCas9 …
    Promoter
    tet-inducible CMV
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139836-plasmid-map-sequence-id-308228

    Enlarge

  13. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid
    #71685
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71685-plasmid-map-sequence-id-322543

    Enlarge

  15. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid
    #71684
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71684-plasmid-map-sequence-id-129672

    Enlarge

  17. pT2-shATRX-GFP

    Plasmid
    #124259
    Purpose
    Expresses shRNA targeting ATRX with a GFP reporter which is driven by the SV40 promoter. Construct has inverted repeats to be used in Sleeping beauty system.
    Depositor
    Maria Castro
    Article
    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii
    Insert
    shATRX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    124259-plasmid-map-sequence-id-248702

    Enlarge

  18. PGL4.11-COL1A1mSMAD

    Plasmid
    #230917
    Purpose
    Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.
    Depositor
    Avi Rosenberg
    Article
    Yang et al Lab Invest. 2024 May;104(5):100336. doi:
    Insert
    Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
    Use
    Luciferase
    Tags
    Luciferase-luc2p
    Expression
    Bacterial and Mammalian
    Mutation
    COL1A1 promoter with mutant potential SMAD bindin…
    Promoter
    COL1A1
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230917-plasmid-map-sequence-id-458138

    Enlarge

  19. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

    Enlarge

  20. Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

    Plasmid
    #135766
    Purpose
    This plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.
    Depositor
    Nadia Mercader Huber
    Article
    Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6
    Insert
    Wilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
    Use
    Zebrafish
    Tags
    Expression
    Mutation
    Promoter
    UAS
    Available since
    Jan. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    135766-plasmid-map-sequence-id-270202

    Enlarge

  21. G protein alpha-s-mCherry

    Plasmid
    #66968
    Purpose
    G protein alpha-s internally tagged with mCherry and EE epitope
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Insert
    G-alpha-s-EE-mCherry (Gnas Discosoma sp., Rat)
    Use
    Tags
    internal EE epitope (residues 189-194 in alpha- s…
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66968-plasmid-map-sequence-id-121428

    Enlarge

  24. G protein alpha-s-mCerulean

    Plasmid
    #66084
    Purpose
    G protein alpha-s internally tagged with mCerulean and EE epitope
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Insert
    G-alpha-s-EE-mCerulean (Gnas Aequorea victoria, Rat)
    Use
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    66084-plasmid-map-sequence-id-118926

    Enlarge

  26. PGL4.11-COL1A1mTEAD

    Plasmid
    #230916
    Purpose
    Comparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.
    Depositor
    Avi Rosenberg
    Article
    Yang et al Lab Invest. 2024 May;104(5):100336. doi:
    Insert
    Human COL1A1 promoter with mutant TEAD elements (COL1A1 Human)
    Use
    Luciferase
    Tags
    Luciferase- luc2p
    Expression
    Bacterial and Mammalian
    Mutation
    mutant potential TEAD bind elements AGGAAT to CTG…
    Promoter
    COL1A1
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230916-plasmid-map-sequence-id-460667

    Enlarge

  31. pαH-S-GSAS-OMICRON.BA.5

    Plasmid
    #213069
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 variant
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    S-GSAS-Omicron.BA5 (S )
    Use
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    CMV
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    213069-plasmid-map-sequence-id-423221

    Enlarge

  34. pαH-S-RRAR/XBB.1.16

    Plasmid
    #213037
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.16 with with furin side intact
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    s -RRAR.XBB.1.16 (S )
    Use
    Tags
    HRV 3C cleavage site (before tags) 8X His tag 2X…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 furin site RRAR …
    Promoter
    CMV
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    213037-plasmid-map-sequence-id-423206

    Enlarge

  35. pαH-S-RRAR-OMICRON.BA.5

    Plasmid
    #213071
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 with with furin side intact
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    pαH-S-RRAR-OMICRON.BA.5 (S )
    Use
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685, furin site inta…
    Promoter
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    213071-plasmid-map-sequence-id-422361

    Enlarge

  50. PGL4.11-COL1A1double mutants

    Plasmid
    #230918
    Purpose
    Double mutation of TEAD and SMAD binding elements in COL1A1 promoter; check the promoter activity.
    Depositor
    Avi Rosenberg
    Article
    Yang et al Lab Invest. 2024 May;104(5):100336. doi:
    Insert
    Human CLO1A1 promoter including SMAD and TEAD mutant elements (COL1A1 Human)
    Use
    Luciferase
    Tags
    Luciferase-luc2p
    Expression
    Bacterial and Mammalian
    Mutation
    COL1A1 promoter with mutant SMAD and TEAD binding…
    Promoter
    COL1A1 with mutant SMAD and TEAD elements
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230918-plasmid-map-sequence-id-460669

    Enlarge

Showing: 2501 - 2550 of 24285 results