171,027 results

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pD2529-CAG-Flag-mouse TGFb3

Plasmid

#180262

Purpose

Mouse full length TGFβ3 expression

Depositor

Timothy Springer

Article

Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

Insert

TGFβ3 (Tgfb3 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pUC_cohNaV1.1_2009aa_IRES2_GFP

Plasmid

#227642

Purpose

Expresses Nav 1.1 and IRES-GFP

Depositor

Dravet Syndrome Foundation Spain

Insert

NaV1.1 (SCN1A Human)

Tags

IRES-GFP

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TOgw-N-mCherry

Plasmid

#223948

Purpose

Gateway cloning of genes of interest with an N-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression

Depositor

Karla Feijs, Roko Žaja

Type

Empty backbone

Tags

mCherry

Expression

Mammalian

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pJUMP27-1A(sfGFP)

Plasmid

#126974

Purpose

Level 1 vector. sfGFP reporter. Origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_NPM1_ALK

Plasmid

#205868

Purpose

Express mEGFP-tagged fusion protein, NPM1_ALK from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

NPM1_ALK (NPM1, ALK Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBbB15a-GFP

Plasmid

#126130

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-TagRFP

Plasmid

#67167

Purpose

CRISPaint universal donor for TagRFP fusion tagging

Depositor

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TagRFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA

Plasmid

#213536

Purpose

Expresses the split transcription factors in mouse liver

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA-HA

Use

AAV

Tags

EGFP and HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -2

Plasmid

#173888

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Lamp1-mTurquoise2

Plasmid

#98828

Purpose

In vivo visualization of lysosomes (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lamp1

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTag-RFP-C-h-Rab11a

Plasmid

#79806

Purpose

Express TagRFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab11a (RAB11A Human)

Tags

TagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC17A9

Plasmid

#131973

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC17A9 (SLC17A9 Human)

Expression

Mammalian

Available Since

Oct. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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rGluA1 (px330:2-guideCas9)

Plasmid

#169437

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-S in pAR9 (rep/cap)

Plasmid

#174539

Purpose

encodes AAV-S capsid for efficient gene delivery to the mammalian cochlea

Depositor

Casey Maguire

Article

Ivanchenko et al Mol Ther Methods Clin Dev. 2021 Mar 29;2

Insert

VP1 of AAV9 containing the AAV-S 7-mer peptide insert

Use

AAV

Expression

Mammalian

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI-CMV-GFP-TREX1 D18N BLAST

Plasmid

#164225

Purpose

lentiviral plasmid for GFP-hTREX1 D18N (catalytically-deficient)

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

D18N

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pmEGFP_a_tubulin_IRES_puro2b

Plasmid

#21042

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCCL-c-MNDU3-CD3D WT cDNA

Plasmid

#198020

Purpose

LV Expression of wildtype CD3D cDNA

Depositor

Donald Kohn

Insert

CD3D WT cDNA

Use

Lentiviral

Expression

Mammalian

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRXG

Plasmid

#113643

Purpose

This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.

Depositor

Jeffrey Barrick

Article

Monk et al ACS Synth Biol. 2016 Sep 20.

Insert

mRFP1-linker-sfGFP

Use

Synthetic Biology

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA neo

Plasmid

#104992

Purpose

This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pmRFP_a_tubulin_IRES_puro2b

Plasmid

#21041

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

pmRFP

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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