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Addgene

We narrowed to 29,328 results for: REP

Showing: 10301 - 10320 of 29328 results
  1. pExp-PpiB

    Plasmid
    #112572
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Type
    Empty backbone
    Use
    Tags
    8xHis-PpiB and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. NM-01

    Plasmid
    #69799
    Purpose
    Bacterial expression of Cas9 with His, MBP, and Strep tags
    Depositor
    Insert
    Cas9
    Use
    Tags
    HIS, MBP, and StrepII
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine (D10A) and Histidine 840 …
    Promoter
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. pcB6-Myc-Mfn2 IYFFT

    Plasmid
    #191953
    Purpose
    Expression of Mfn2 protein, in which the C-terminus, after the transmembrane domain, is replaced by a stretch of hydrophobic amino acids (IYFFT)
    Depositor
    Insert
    Myc-Mfn2-IYFFT
    Use
    Tags
    Expression
    Mammalian
    Mutation
    C- terminus replaced by the stretch of hydrophobi…
    Promoter
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. N1-LOC8R

    Plasmid
    #66896
    Purpose
    Leader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial targeting sequence compatible with any N1 vector. Internal restriction sites enable modular subcloning of 1x, 2x, 3x or 4x presequences.
    Depositor
    Insert
    LOC8R
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pExp-DsbA(SS)

    Plasmid
    #112563
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Type
    Empty backbone
    Use
    Tags
    8xHis-DsbA(SS) and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. psiCHECK-2 GATD1 Alu_Mut1

    Plasmid
    #205470
    Purpose
    Luciferase reporter for Alu Domain Activity
    Depositor
    Insert
    Mutant 1 GATD1 Alu Domain
    Use
    Luciferase and RNAi
    Tags
    Expression
    Mutation
    Mutated the entire structure of the alu domain wi…
    Promoter
    T7
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pSG7084 pG46-46

    Plasmid
    #232621
    Purpose
    pG46 derivative containing two replacement cassette sites (siteA and siteB) for gap-replacement-based chemical modification(s).
    Depositor
    Type
    Empty backbone
    Use
    Precursor plasmid to generate modified dna circles
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. 1203T-CMV-Cas7.1-NLS-bGHpolyA

    Plasmid
    #221540
    Purpose
    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain
    Depositor
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. 1203W-CMV-Cas7.1-7.2-bGHpolyA

    Plasmid
    #220961
    Purpose
    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and Cas7.2 domains from DiCas7-11, deactivated (truncated from DiCas7-11 effector)
    Depositor
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. pDONR223 Rab25-STOP

    Plasmid
    #228034
    Purpose
    DONR vector for shuttling Rab25 into DEST vector. Contains a STOP codon.
    Depositor
    Insert
    Rab25 (RAB25 Human)
    Use
    Gateway donr
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. pHAGE mCherry-Rab25

    Plasmid
    #228039
    Purpose
    Mammalian lentiviral expression of mCherry-labeled Rab25.
    Depositor
    Insert
    Rab25 (RAB25 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. R6K_BAD_GFP-mCherry_ChInt++

    Plasmid
    #187393
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette encoding for arabinose-inducible GFP and mCherry
    Depositor
    Insert
    GFP, mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. R6K_BAD_GFP-mCherry_ChInt--

    Plasmid
    #187394
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette encoding for arabinose-inducible GFP and mCherry
    Depositor
    Insert
    GFP, mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 10301 - 10320 of 29328 results