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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31,561 results for: ide

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Showing: 10301 - 10320 of 31561 results

  11. pCMV6-AC EJ7-GFP

    Plasmid
    #113617
    Purpose
    Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP
    Depositor
    Jeremy Stark
    Article
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Insert
    EJ7-GFP variant of EGFP
    Expression
    Mammalian
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    113617-plasmid-map-sequence-id-218254

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  12. pcDNA3-Lyn-dPlcR

    Plasmid
    #181832
    Purpose
    Genetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).
    Depositor
    Jin Zhang
    Article
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Insert
    Lyn-PlcR
    Tags
    N-terminal targeting sequence from Lyn kinase, dd…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181832-plasmid-map-sequence-id-364041

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  18. PX551

    Plasmid
    #60957
    Purpose
    pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.
    Depositor
    Feng Zhang
    Article
    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Tags
    HA
    Expression
    Mammalian
    Promoter
    pMecp2
    Available Since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    60957-plasmid-map-sequence-id-181490

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  19. pGL3-NFAT luciferase

    Plasmid
    #17870
    Purpose
    3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue
    Depositor
    Jerry Crabtree
    Article
    Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.
    Insert
    3x NFAT binding sequence (IL2 )
    Use
    Luciferase
    Tags
    IL2 promoter and Luciferase
    Expression
    Mammalian
    Promoter
    IL-2 promoter
    Available Since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only
    17870-plasmid-map-sequence-id-325133

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Showing: 10301 - 10320 of 31561 results