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pSico shMAP3K2 human

Plasmid

#85656

Purpose

expression of shRNA targeting human MAP3K2

Depositor

Julien Sage

Article

Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

Insert

shRNA targeting 3'UTR of MAP3K2 (MAP3K2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMiR-CIP2A-miR-375-mutC&D

Plasmid

#53712

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site C&D (refer t…

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutE

Plasmid

#53710

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site E (refer to cita…

Available Since

July 11, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutA&B

Plasmid

#53711

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site A&B (refer t…

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutD

Plasmid

#53709

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site D (refer to cita…

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutC

Plasmid

#53708

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site C (refer to cita…

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutB

Plasmid

#53707

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site B (refer to cita…

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutA

Plasmid

#53706

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site A (refer to cita…

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
pBIG2abc_hsHAUScomplex_FL

Plasmid

#202203

Purpose

Baculoviral transfer vector to co-express 8 subunits of human augmin complex

Depositor

Thomas Surrey

Article

Ustinova et al J Cell Sci. 2023 Jun 15;136(12):jcs26109

Inserts

HAUS1 (HAUS1 Human, codon-optimized form for expression in Trichoplusia ni cells)
HAUS3 (HAUS3 Human, codon-optimized form for expression in Trichoplusia ni cells)
HAUS2 (HAUS2 Human, codon-optimized form for expression in Trichoplusia ni cells)
HAUS6 (HAUS6 Human, codon-optimized form for expression in Trichoplusia ni cells)
HAUS7 (HAUS7 Human, codon-optimized form for expression in Trichoplusia ni cells)
HAUS4 (HAUS4 Human, codon-optimized form for expression in Trichoplusia ni cells)
HAUS5 (HAUS5 Human, codon-optimized form for expression in Trichoplusia ni cells)
HAUS8 (HAUS8 Human, codon-optimized form for expression in Trichoplusia ni cells)

Tags

-mGFP-TEV-TwinStrep and His-tag (6x)

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pRS415-IVa

Plasmid

#31460

Depositor

Ana Kitazono

Article

Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

Insert

linker

Expression

Worm

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pRS415-IVb

Plasmid

#31461

Depositor

Ana Kitazono

Article

Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

Insert

linker

Expression

Yeast

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pRS413-IVa

Plasmid

#31458

Depositor

Ana Kitazono

Article

Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

Insert

linker

Expression

Yeast

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pRS413-IVb

Plasmid

#31459

Depositor

Ana Kitazono

Article

Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

Insert

linker

Expression

Yeast

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pRS316-IVb

Plasmid

#31457

Depositor

Ana Kitazono

Article

Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

Insert

linker

Expression

Yeast

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pGFPns-reporter

Plasmid

#35626

Depositor

Howard Gu

Article

Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

Type

Empty backbone

Use

Reporter

Tags

GFPns

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pEC1.2/EC1.1_Cas9i_TagRFP

Plasmid

#227249

Purpose

CRISPR plasmid with Cas9i expressed under egg cell promoter - TagRFP seed selection

Depositor

Christian Fankhauser

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

pEC1.2/EC1.1

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pPT24

Plasmid

#184419

Purpose

Transformation of mitochondrial DNA in yeast

Depositor

Martin Ott

Article

Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

Type

Empty backbone

Expression

Yeast

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pRS316-IVa

Plasmid

#31456

Depositor

Ana Kitazono

Article

Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

Insert

linker

Expression

Yeast

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
MLRV2: NF-kb-SMAD-DBE-P53-AP1

Plasmid

#124536

Purpose

Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Inserts

5 copies of the NF-kb DNA binding motif
7xSMAD_RE::FLuc
3xDBE_BE::Renilla (FOXO)
2xP53_RE::NLuc
6xAP-1_RE::GrRenilla

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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hfCas12Max

Plasmid

#195336

Purpose

vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

human codon-optimized hfCas12Max

Tags

3xFlag

Expression

Mammalian

Mutation

N243R, E336R, D892R

Promoter

CBh, CMV, hU6

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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