We narrowed to 19,454 results for: Tec
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Plasmid#140643PurposeAcetylcholine sensor for sindbis virusDepositorArticleInsertAch sensor V9
ExpressionMammalianPromoterSP6Available SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph15-eGFP-CCR5TC
Plasmid#135528PurposeEncodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph15
TagseGFPExpressionMammalianPromoterCAGAvailable SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-3xMBT_D355N
Plasmid#46988PurposeInducible expression of 3xMBT (D355N) from L3MBTL1 as a GST fusion protein in E.coliDepositorInsertTriple MBT domain from human L3MBTL1 (L3MBTL1 Human)
TagsGST and PreScission cleavage siteExpressionBacterialMutationMutation aspartic acid 355 to asparagine (D355N);…PromotertacAvailable SinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRaGE Pyl TAG GFP Y35TAG
Plasmid#160041PurposeEncodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.DepositorInsertsPyrrolysyl tRNA synthetase (Methanosarcina mazei)
Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
deGFP
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationChanged tyrosine 35 to TAG stop-codon (Site numbe…PromoterP70b promoter and T500 terminator, Vibrio natrieg…Available SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSynapsin-iAchSnFR
Plasmid#140644PurposeAcetylcholine sensor for lenti virusDepositorArticleInsertAch sensor V9
UseLentiviralPromoterhsynapsinAvailable SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF208
Plasmid#101549PurposeDonor Vector containing ZNF208 transcription factor, part of the Human TFome CollectionDepositorInsertZNF208 (ZNF208 Human)
UseGateway shuttling vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Circular 200,100 (RAB7A)
Plasmid#170118PurposeAAV vector carrying a guide RNA targeting the human RAB7A mRNADepositorAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJFA344C7
Plasmid#49236PurposeTET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cellsDepositorInsertTALE-TET1CD Backbone (TET1 Synthetic, Human)
UseTALEN; Tale-tet1 expression vectorPromoterEF1aAvailable SinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-shMcu-1
Plasmid#181868PurposeExpresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoterDepositorInsertmitochondrial calcium uniporter (Mcu Mouse)
UseAAV, Adenoviral, and Mouse TargetingTagshrGFPExpressionMammalianPromoterU6Available SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl2-Cb5
Plasmid#18000DepositorInsertER targeted Bcl-2 (BCL2 Human)
TagsGFPExpressionMammalianMutationBcl-2's C terminal targeting sequence replac…PromoterCMVAvailable SinceJune 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero45
Plasmid#187931PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero45DepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF280C
Plasmid#88658PurposeDonor Vector containing ZNF280C transcription factor, part of the Human TFome CollectionDepositorInsertZNF280C (ZNF280C Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl2-Maob
Plasmid#18001DepositorInsertMitochondria-targeted Bcl-2 (BCL2 Human)
TagsGFPExpressionMammalianMutationBcl-2's C terminal replaced with mitochondri…PromoterCMVAvailable SinceJune 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
hU6-pegRNA-EF-1α-Cre Template
Plasmid#196036PurposeThe template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre.DepositorTypeEmpty backboneExpressionMammalianPromoterhU6 and EF-1-alphaAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-xFNLS-P2A-Puro
Plasmid#110872PurposeLentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)DepositorInsertxFNLS(3.7)
UseLentiviralTags3X FLAGMutationD10A, A262T, R324L, S409I, E480K, E543D, M694I, E…PromoterEF1sAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1RA-P2A-GFP-PGK-Puro
Plasmid#110866PurposeLentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertCas9-HF1RA
UseLentiviralTagsFLAGMutationR661A, Q695A, Q926A, and NLS sequence at the N-te…PromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
U6-hGRIN2B-CAG-ps-SpCas9
Plasmid#102851PurposeA single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editingDepositorInsertsp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B Synthetic, Human, S. pyogenes)
UseCRISPRTags3X Flag and NLSExpressionMammalianPromoterU6 promoterAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCU-EGFP
Plasmid#181866PurposeExpresses GFP-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoterDepositorInsertmitochondrial calcium uniporter (Mcu Mouse)
UseAAV and AdenoviralTagsEGFPExpressionMammalianPromoterCaMKIIaAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
Polymerase-unfused click editor - pCMV-T7-PCV2-nCas9 (LM2702)
Plasmid#208961PurposePolymerase-unfused click editor comprised of PCV2 fused to nCas9, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
RNASEL gRNA (BRDN0001146030)
Plasmid#77053Purpose3rd generation lentiviral gRNA plasmid targeting human RNASELDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only