We narrowed to 18,496 results for: Met

1. MAC-SARS-CoV-2 NSP1

   Plasmid

   #158352

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 NSP1 (ORF1ab )
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. SEPTOPO

   Plasmid

   #64944

   Purpose

   source of SEP (Super Ecliptic pHluorin) sequence

   Depositor

   Jeremy Henley

   Article

   Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

   Insert

   SEP
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pPMVAd36

   Plasmid

   #98302

   Purpose

   Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   PCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

   Expression

   Yeast

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXWJ109HgAmp30E

   Plasmid

   #123149

   Purpose

   Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J109, PmerT, PhrpLE

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT11

   Plasmid

   #98308

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

   Insert

   TRPL3ClLIS1>TRPL41B
   

   Expression

   Yeast

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJCC_075 AsCas12a D908A

   Plasmid

   #171668

   Purpose

   For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   AsCas12a D908A
   

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed aspartate 908 to alanine

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZ_lacO-cP-AOX1-NpgA

   Plasmid

   #126731

   Purpose

   yeast plasmid overexpressing NpgA

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   npgA
   

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   G951A,C957A

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT3TS-HiFi-nCas9n-A72

   Plasmid

   #125646

   Purpose

   micro-injection of poly-adenylated HiFi-nCas9n RNA

   Depositor

   Jason Berman

   Article

   Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

   Insert

   HiFi-nCas9n
   

   Use

   Rna in vitro transcription vector

   Mutation

   R691A from wild-type Cas9

   Promoter

   T3

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEAST-pBen-sfGFP

   Plasmid

   #114598

   Purpose

   "Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Promoter

   PBen

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2ADW-lox-mCherry-hyBRET-ERK-NLS

    Plasmid

    #170290

    Purpose

    For generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.

    Depositor

    Michiyuki Matsuda

    Article

    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

    Insert

    lox-mCherry-lox-hyBRET-ERK-NLS
    

    Expression

    Mammalian

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. 35S::Cel1SP-GeNL/pCAMBIA1301

    Plasmid

    #190069

    Purpose

    Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

    Depositor

    Takeharu Nagai

    Article

    Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

    Insert

    Cel1 signal peptide-Green-enhanced Nano-lantern
    

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBEAST-BenR

    Plasmid

    #114597

    Purpose

    "Strong constitutive expression of transcription factor, BenR, for cell-free expression"

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    BenR
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Promoter

    OR2-OR1-Pr

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)

    Plasmid

    #139788

    Purpose

    Vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

    Depositor

    Arneet Saltzman

    Article

    Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

    Type

    Empty backbone

    Tags

    6xHis (cleavable by TEV protease) and GST (cleava…

    Expression

    Bacterial

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. FH-TET3-CXXCmut-pEF

    Plasmid

    #127895

    Purpose

    Expresses ten-eleven-translocation 3 (TET3) containing CXXC mutation 80THQ82 to AAA

    Depositor

    Patrick Hogan, Anjana Rao

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    TET3-CxxCmut (TET3 Human)
    

    Tags

    HA and Flag

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-X1-Neo-NDUFB2

    Plasmid

    #139841

    Purpose

    Lentiviral expression of human NDUFB2

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    NDUFB2 (NDUFB2 Human)
    

    Use

    Lentiviral

    Available Since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAc5.1B-lambdaN-HA-DmPAN3-W707AG711E-dsRNAres_Y

    Plasmid

    #148087

    Purpose

    Insect Expression of DmPAN3-W707AG711E-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN3-W707AG711E-dsRNAres (CG11486 Fly)
    

    Expression

    Insect

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAC-SARS-CoV-2 NSP2

    Plasmid

    #158353

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 NSP2 (ORF1ab )
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pegRNA_HEK4_20-7

    Plasmid

    #201974

    Purpose

    Express a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology arm

    Depositor

    Scot Wolfe

    Article

    Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

    Insert

    pegRNA_HEK4_20-7
    

    Expression

    Mammalian

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. MAC-SARS-CoV-2 Orf10

    Plasmid

    #158380

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 Orf10 (ORF10 )
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pHIE050 ZF43x0 mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138731

    Purpose

    Integration Vector, assembled with pHIE042 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x0
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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