We narrowed to 13,854 results for: GAN

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pFRT-TO-RPB1-His K710,719R CRres si2,4R

Plasmid

#139407

Purpose

Dox-inducible, K710, 719R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Tags

6xHis

Expression

Mammalian

Mutation

K710,719R

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 C-t

Plasmid

#89981

Purpose

Expresses the GFP-tagged C-terminus of ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-182

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM138-mCherry

Plasmid

#41630

Depositor

Joseph Gleeson

Article

Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

Insert

Transmembrane Protein 138 (TMEM138 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only
FB013

Plasmid

#119710

Purpose

TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

Plasmid

#169166

Purpose

Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

Insert

3xFlag-6His-nsp15 (ORF1ab Synthetic, SARS-CoV-2)

Tags

3xFlag-6His

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV ShadowG-AURKA-mTurq2

Plasmid

#157768

Purpose

Expression of AuroraA kinase biosensor under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2 and shadowG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-hINO80 HSA (212-526)

Plasmid

#29443

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 212-526 (a.a.)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
IGFALS-bio-His

Plasmid

#52180

Purpose

Expresses full-length Insulin-like growth factor-binding protein complex ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

IGFALS (IGFALS Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-Dendra2-Puro

Plasmid

#169217

Purpose

Targeting vector backbone to support a knock-in of Linker-Dendra2-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked Dendra2-P2A-Puro

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEMS2272

Plasmid

#111898

Purpose

ssAAV genome with Ple342 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple342 (TUBB3 Human)

Use

AAV

Promoter

Ple342 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-hINO80C (1224-1556)

Plasmid

#29445

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 1224-1556 (a.a.)

Available Since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-bio-myc-mCE 2-210

Plasmid

#112715

Purpose

Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Tags

biotinylation signal peptide (Tagwerker et al. 20…

Expression

Mammalian

Mutation

Silent mutation at Arg 132 (CGT to CGC), deleted …

Promoter

CMV

Available Since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3c_SUD-N

Plasmid

#156469

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-N protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-N (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3c_SUD-M

Plasmid

#156470

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-M (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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LADL Bridge + Promoter Only Target

Plasmid

#127669

Purpose

Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

CRY2-HA-2A-mCherry and gRNAs 115 and 117

Use

CRISPR

Tags

HA

Expression

Mammalian

Promoter

EF1a and hU6

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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zgc:111821_R (OZ598)

Plasmid

#35238

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zgc:111821 (zgc:111821 Zebrafish)

Use

Zebrafish targeting

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
zgc:111821_L (OZ597)

Plasmid

#35237

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zgc:111821 (zgc:111821 Zebrafish)

Use

Zebrafish targeting

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
CACNA1F_R (OZ508)

Plasmid

#27183

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting cacna1f (cacna1f Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only
Encephalopsin (Opn3)_L (OZ541)

Plasmid

#28066

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting Encephalopsin (Opn3) (opn3 Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
Encephalopsin (Opn3)_R (OZ542)

Plasmid

#28067

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting Encephalopsin (Opn3) (opn3 Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only