161,488 results

1. pLV-tetO-myc T58A

   Plasmid

   #19763

   Depositor

   Konrad Hochedlinger

   Article

   Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

   Insert

   c-myc (MYC Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   T58A

   Promoter

   Available since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcsII-LMP7-flag

   Plasmid

   #86773

   Purpose

   C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   immunoproteasome beta subunit 5 (LMP7) (PSMB8 Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-ccdB-3xHA

   Plasmid

   #214986

   Purpose

   Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xHA tag

   Depositor

   Wade Harper

   Type

   Empty backbone

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

   Plasmid

   #199349

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-SUMOEu

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. APneg-VAMP1b

   Plasmid

   #168008

   Purpose

   negative selection PACE Accessory Plasmid

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   VAMP1(T29-K89)-T7PAP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. APneg-VAMP1c

   Plasmid

   #168009

   Purpose

   negative selection PACE Accessory Plasmid

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   VAMP1(T29-K89)-T7PAP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lifeact-3xmNG::HIS3

   Plasmid

   #227596

   Purpose

   Integration vector for expressing Lifeact-3xmNeonGreen

   Depositor

   Bruce Goode

   Article

   Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

   Insert

   Lifeact-3xmNeonGreen::HIS3
   

   Use

   Tags

   3xmNeonGreen

   Expression

   Yeast

   Mutation

   Truncated ABP140 (aa 1-17 only)

   Promoter

   Abp140

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP11818-pAAV-AiE2004m_3xC2-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN1818)

   Plasmid

   #164458

   Purpose

   AiE2004m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minCMV

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLHCX-FLAG-mPKM1

   Plasmid

   #44240

   Depositor

   Lewis Cantley, Matthew Vander Heiden

   Article

   Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

   Insert

   Pyruvate Kinase M1 (Pkm Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

10. H2B-mGarnet2

    Plasmid

    #104312

    Purpose

    codes for a fusion of fluorescent protein mGarnet2 and human histon 2B (H2B), makes the H2B visible in live cells

    Depositor

    Ulrich Nienhaus

    Article

    Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

    Insert

    H2B-mGArnet2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNS35 (multiAsCas12a piggyBac)

    Plasmid

    #217336

    Purpose

    piggyBac expression of multiAsCas12a

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    multiAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    R1226A/E174R/S542R/K548R

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. LT1_36_QUEEN-2m

    Plasmid

    #177708

    Purpose

    Plasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    pTEF1-QUEEN2m
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. 6xHis-MBP-huTsCas12a

    Plasmid

    #174687

    Purpose

    Expresses 6xHis-MBP tagged TsCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huTsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

    Plasmid

    #31476

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV d14 GFP donor EF1s I-SceI
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    GFP has 14 amino acids deleted from c terminus

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK-FLAG-UbxD8

    Plasmid

    #53777

    Purpose

    express FLAG-tagged human UbxD8 gene

    Depositor

    Yihong Ye

    Article

    Xu et al J Biol Chem. 2013 Jun 21;288(25):18068-7

    Insert

    UbxD8 (FAF2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

16. FKBP-mEGFP-YAP-MAML2

    Plasmid

    #217612

    Purpose

    part A of YAP-MAML2 SPARK-OFF

    Depositor

    Xiaokun Shu

    Article

    Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

    Insert

    FKBP-mEGFP-YAP-MAML2 (YAP1, MAML2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC1_oROS-HT

    Plasmid

    #216413

    Purpose

    Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. plk1 T210D

    Plasmid

    #132964

    Purpose

    To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

    Depositor

    Michael Yaffe

    Article

    Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

    Insert

    polo-like kinase 1 (PLK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T210D

    Promoter

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. p-dCas9-HDAC1-Hygro

    Plasmid

    #104409

    Purpose

    transient expression of dCas9-HDAC1 fusion protein

    Depositor

    Stephan Beck

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPAICS-EGFP

    Plasmid

    #99108

    Purpose

    Expresses human PAICS-EGFP fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    PAICS (PAICS Human)
    

    Use

    Tags

    EGFP (enhanced green fluorescent protein)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge