8,964 results

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pHAGE-KRAS-G12D

Plasmid

#116423

Purpose

Lentiviral expression of KRAS G12D

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

G12D

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-gDA3m

Plasmid

#208698

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Has Service

AAV1

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3m

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-Flag-c-Myc

Plasmid

#102626

Purpose

Expresses c-Myc in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

MYC proto-oncogene, bHLH transcription factor (MYC Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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Ub-G76V-GFP

Plasmid

#11941

Depositor

Nico Dantuma

Article

Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

Insert

Ub-G76V-GFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Glycine 76 …

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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pET28a-EGFP-CNA35

Plasmid

#61603

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with EGFP

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

EGFP and CNA35

Tags

His tags

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.TBG.PI.eGFP.WPRE.bGH

Plasmid

#105535

Purpose

AAV expression of EGFP from TBG promoter

Depositor

James M. Wilson

Has Service

AAV8

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

TBG

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-pHluorin_M153R-CD63

Plasmid

#172117

Purpose

Fluorescent reporter for CD63-positive exosome secretion

Depositor

Alissa Weaver

Article

Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

Insert

CD63 (CD63 Human)

Use

Lentiviral

Tags

pHluorin_M153R in a small extracelluar loop of CD…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-rab11 WT

Plasmid

#12674

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Tags

GFP and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Plasmid

#83841

Purpose

Fluorescent probe for G1/S transition and M/G1 transition, one of two plasmids for the FUCCI4 cell cycle reporter

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCG-Hc∆18

Plasmid

#84817

Purpose

Measles virus hemagglutinin expressing plasmid

Depositor

Jakob Reiser

Article

Marino et al Gene Ther. 2015 Mar;22(3):280-5. doi: 10

Insert

Measles virus H glycoprotein

Expression

Mammalian

Promoter

CMV IE

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pRT043-CLYBL-DDdcas9-VPH

Plasmid

#158091

Purpose

Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

Depositor

Martin Kampmann

Article

Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

Insert

CLYBL-CAG-DDdCas9VPH-T2A-GFP

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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Watermelon backbone

Plasmid

#155258

Purpose

Can be used to generate a Watermelon library

Depositor

Joan Brugge, Aviv Regev

Article

Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

Plasmid

#111568

Purpose

Expresses a plasma membrane calcium pump hPMCA2w/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Has Service

AAV5

Insert

Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)

Use

AAV

Tags

mCherry

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET-MBP-mSA2

Plasmid

#52319

Purpose

Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

Depositor

Sheldon Park

Article

Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

Insert

monomeric streptavidin 2

Tags

FLAG, His, MBP, and TEV

Expression

Bacterial

Mutation

S25H mutation compared to streptavidin

Promoter

T7

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-GalT

Plasmid

#11929

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pCR4-24XMS2SL-stable

Plasmid

#31865

Depositor

Robert Singer

Article

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

24xMS2 Stem Loops - Stable

Available Since

Aug. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pY094

Plasmid

#84743

Purpose

Expresses huAsCpf1-T2A-GFP and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
GFP

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-HA-hYTHDC1

Plasmid

#85167

Purpose

Expression of FLAG-HA-tagged human YTHDC1

Depositor

Samie Jaffrey

Article

Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

Insert

YTHDC1 (YTHDC1 Human)

Tags

Flag and Ha

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Tet2

Plasmid

#60939

Purpose

Expresses Tet2 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 2 (Tet2 Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-shYAP1

Plasmid

#27368

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

shYAP1

Expression

Mammalian

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pLVX-EGFP-IRES-puro

Plasmid

#128652

Purpose

mammalian expression of EGFP with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

EGFP

Use

Lentiviral

Tags

N/A

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG3K(1-10)

Plasmid

#157993

Purpose

Lentiviral vector of constitutive expression of mNeonGreen3K(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

pSFFV_mNG3K(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

G27D, D42E, M128I, K153M, T170I

Promoter

SFFV

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-Luc2-P2A-tdTomato

Plasmid

#72486

Purpose

Express Luc2 and tdTomato under EF1 promoter

Depositor

Kazuhiro Oka

Insert

Luc2-P2A-tdTomato

Use

Lentiviral

Expression

Mammalian

Promoter

EF1

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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ISceI-GR-RFP

Plasmid

#17654

Depositor

Tom Misteli

Article

Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

Insert

I-SceI-GRLBD (SCEI, Nr3c1 Rat, Budding Yeast)

Tags

mRFP

Expression

Mammalian

Mutation

C655G mutation in the glucocorticoid receptor (se…

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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ML1Nwt_in_pEGFP-C1

Plasmid

#67797

Purpose

Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain wildtype version) fused to EGFP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pET21a-alpha-synuclein

Plasmid

#51486

Purpose

Inducible expression of human alpha-synuclein in E.coli

Depositor

Michael J Fox Foundation MJFF

Insert

alpha-synuclein (SNCA Human)

Expression

Bacterial

Promoter

T7

Available Since

March 31, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pScaf

Plasmid

#111401

Purpose

Phagemid for producing DNA origami scaffolds, derived from pUC18 and M13mp18

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCasSA

Plasmid

#98211

Purpose

CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

Depositor

Quanjiang Ji

Article

Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-ATG8(416)/GFP-AUT7(416)

Plasmid

#49425

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

Depositor

Daniel Klionsky

Article

Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

ATG8

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

Plasmid

#199390

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Tags

14xHis-Avi-SUMOEu

Expression

Bacterial

Promoter

T5-LacO

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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