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We narrowed to 18,037 results for: multi

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Showing: 1041 - 1060 of 18037 results

  2. Opto-CRAC version 1

    Plasmid
    #101245
    Purpose
    Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling
    Depositor
    Yubin Zhou
    Publication
    He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LOV2 and STIM1 cytosolic fragment
    Tags
    mCherry
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    101245-plasmid-map-sequence-id-209272

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  3. pCI-neo FlucSM EGFP

    Plasmid
    #90171
    Purpose
    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation
    Depositor
    Franz-Ulrich Hartl
    Publication
    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLuc EGFP
    Expression
    Mammalian
    Mutation
    R188Q in FLuc
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    90171-plasmid-map-sequence-id-226523

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  4. pSR657

    Plasmid
    #65214
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Publication
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-2 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    p10 and polyhedrin
    Available since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    65214-plasmid-map-sequence-id-116341

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  5. p46Cpf1-OP2

    Plasmid
    #98592
    Purpose
    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.
    Depositor
    Qiong Wu
    Publication
    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    araBAD
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    98592-plasmid-map-sequence-id-201329

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  11. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

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  12. pFC333

    Plasmid
    #87844
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker
    Depositor
    Uffe Mortensen
    Publication
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    ble (bleomycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    87844-plasmid-map-sequence-id-174620

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  14. FLX1.8NFATGFPpd2HS4

    Plasmid
    #169096
    Purpose
    Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation
    Depositor
    Geoffrey Owens
    Inserts
    NFAT enhancer (from IL-4 promoter)
    Destabilized green fluorescent protein
    Use
    Lentiviral
    Promoter
    NFAT enhancer + synthetic TATA box
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169096-plasmid-map-sequence-id-338033

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  16. mCherry-CRY2-5ptaseOCRL

    Plasmid
    #66836
    Purpose
    Expression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.
    Depositor
    Pietro De Camilli
    Publication
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5ptaseOCRL (OCRL Human)
    Tags
    mCherry-CRY2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    66836-plasmid-map-sequence-id-116702

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Showing: 1041 - 1060 of 18037 results