We narrowed to 18,496 results for: Met

1. pWP1_mFTCD

   Plasmid

   #102317

   Purpose

   expression of mouse cDNA of FTCD, refractory to the guides in plasmids 1,2

   Depositor

   David Sabatini

   Article

   Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   Ftcd (Ftcd Mouse)
   

   Use

   Lentiviral

   Promoter

   EF1alpha

   Available Since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJC1-Rap2A-3xFLAG

   Plasmid

   #87972

   Purpose

   Expression of Rap2A in mammalian cells

   Depositor

   David Sabatini

   Article

   Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. JM3 [JBEI-6891]

   Plasmid

   #89788

   Purpose

   Broad host range vector pBBR1-MCS2 containing 'tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli) under control of BAD promoter

   Depositor

   Harry Beller

   Article

   Muller et al Appl Environ Microbiol. 2013 Jul;79(14):

   Insert

   tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli)
   

   Expression

   Bacterial

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGREAT4

   Plasmid

   #170892

   Purpose

   GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   pG10-90

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. KHBD00486

   Plasmid

   #39591

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG5965 (woc Fly)
   

   Use

   Gateway donr vector

   Promoter

   none

   Available Since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

6. GST-PRMT9 L182A/D183A/I184A/G185A

   Plasmid

   #67608

   Purpose

   bacterial expression of catalytically inactive GST-PRMT9 LDIG(182-185)AAAA mutant

   Depositor

   Steven Clarke

   Article

   Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

   Insert

   PRMT9 (PRMT9 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   catalytic mutant LDIG(182-185)AAAA

   Promoter

   tac

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pEGFP-C1X-Nup88-mut

   Plasmid

   #87337

   Purpose

   To express human Nup88 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP88 (NUP88 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   shRNA resistant changes

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pM117: VEGFA array presgRNA

   Plasmid

   #166869

   Purpose

   U6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Insert

   Human VEGFA targeting array presgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSECB

   Plasmid

   #106305

   Purpose

   Express spCas9 with sgRNA, blast resistance

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CRISPR V2 sgShmt2_2

    Plasmid

    #106315

    Purpose

    Express Cas9 and sgRNA targeting mShmt2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting mShmt2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET51b(+)-NADPH-LUCID-2 (C50 29 nM)

    Plasmid

    #117909

    Purpose

    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nM

    Depositor

    Kai Johnsson

    Article

    Yu et al Science. 2018 Sep 14;361(6407):1122-1126

    Insert

    SNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    R98A on eDHFR

    Promoter

    T7

    Available Since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLPC myc-mRAP1 I312R

    Plasmid

    #73137

    Purpose

    Expression of myc-tagged mRAP1 with single point mutatoin I312R

    Depositor

    Agnel Sfeir

    Article

    Yeung et al Cell Rep. 2013 Jun 27;3(6):1847-56. doi:

    Insert

    Rap1 (Terf2ip Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    isoleucine changed to an arginine at amino acid 3…

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pET28a-His6-OAZ-GSY-SPM-Ctag

    Plasmid

    #163613

    Purpose

    Expresses truncated human Ornithine Decarboxylase Antizyme fused to SPM in bacterial cells for calcium-induced covalent coupling to Ornithine Decarboxylase.

    Depositor

    Mark Howarth

    Article

    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

    Insert

    OAZ-GSY-SPM
    

    Tags

    C-tag and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSET FLIPglu-600uDelta13 Aphrodite

    Plasmid

    #13570

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta13 Aphrodite
    

    Tags

    Aphrodite and ECFP

    Expression

    Bacterial

    Mutation

    Fluorophore-derived linker shortened. F16A mutati…

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. OphA-delta C6

    Plasmid

    #127679

    Purpose

    Bacterial expression of His-tagged OphA-delta C6

    Depositor

    James Naismith

    Article

    Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

    Insert

    OphA-delta C6
    

    Tags

    His8

    Expression

    Bacterial

    Mutation

    delta C6

    Available Since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. KHBD00359

    Plasmid

    #34479

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG7839 (CG7839 Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

17. As1

    Plasmid

    #123159

    Purpose

    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J117, ParsR, PhrpLE

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MolBC

    Plasmid

    #92210

    Purpose

    Expression of H. influenzae MolBC (ABC Transporter) in E. coli

    Depositor

    Douglas Rees

    Article

    Pinkett et al Science. 2007 Jan 19;315(5810):373-7. Ep

    Inserts

    MolC (HI1471 )
    MolB (HI1470 )
    

    Tags

    8x Histidine Tag and Enterokinase Cleavage Site b…

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Neo-PTEN

    Plasmid

    #16411

    Depositor

    Bert Vogelstein

    Article

    Kohli et al Nucleic Acids Res. 2004 . 32(1):e3.

    Insert

    PTEN Neo targeting (PTEN Human)
    

    Use

    AAV; Adeno-associated viral

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

20. A102 ΔAmpH Δ2x+ve

    Plasmid

    #129484

    Purpose

    Expression of Glo3 ΔAmpH lacking the COPI-binding motif in E.Coli

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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