We narrowed to 59,318 results for: %s

1. pFUW-TetO-Txnip

   Plasmid

   #178437

   Purpose

   doxycycline-inducible expression of Mouse Txnip in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

   Insert

   Txnip (Txnip Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL-p21UTRm2

   Plasmid

   #20879

   Depositor

   Robert Blelloch

   Article

   Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

   Insert

   p21 3'UTR site 2 mutation (Cdkn1a Mouse)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Predicted miRNA binding site 2 (Position ~1100) G…

   Available Since

   March 12, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pUPD2 PhiC31 attP:TMtb:P35S:attB (GB1494)

   Plasmid

   #160577

   Purpose

   35S promoter sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB)

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   PhiC31 PB (attP:TMtb:P35S:attB)
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. FnoCas12a-DR-cr in PBCSK-

   Plasmid

   #126642

   Purpose

   Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   FnoCas12a Full Length Direct Repeat crRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 Promoter

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET19b-hMED9

   Plasmid

   #15365

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Paoletti et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

   Insert

   hMED9 (MED9 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   N/A

   Available Since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

6. HEL binding scFv D44.1

   Plasmid

   #111718

   Purpose

   Yeast Surface Display of D44.1 as a reference starting point for design

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   D44.1
   

   Tags

   cmyc tag

   Expression

   Yeast

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVL94 - pOp4:VND7

   Plasmid

   #116016

   Purpose

   destination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-induction

   Depositor

   Thomas Greb

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
   

   Expression

   Plant

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-FluoSTEP-EKAR

   Plasmid

   #181858

   Purpose

   Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-EKAR
   

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. 49535-sgFMR1_E3B

   Plasmid

   #157783

   Purpose

   Expresses a sgRNA targeting the 3rd exon of human FMR1 gene

   Depositor

   Xinyu Zhao

   Article

   Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

   Insert

   FMR1 (FMR1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MO70-hJunctate - EF-mutant

    Plasmid

    #79597

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

    Insert

    junctate (ASPH Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    77DAD79 to 77AAA79

    Promoter

    CMV

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

11. dmBACCS2VL-IRES-dOrai-IRES-GFP

    Plasmid

    #72897

    Purpose

    Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2VL-IRES-dOrai-IRES-GFP
    

    Tags

    IRES-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. LjUBQ:MtGLV6:T35s

    Plasmid

    #164741

    Purpose

    Overexpression of Peptide coding gene (Binary vector)

    Depositor

    Wolf Scheible

    Article

    Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

    Insert

    Medtr3g095180.1
    

    Tags

    Terminator 35S

    Expression

    Plant

    Promoter

    Lotus japonicus Ubiquitin

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. LjUBQ:MtGLV2:T35s

    Plasmid

    #164740

    Purpose

    Overexpression of Peptide coding gene (Binary vector)

    Depositor

    Wolf Scheible

    Article

    Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

    Insert

    Medtr5g012400.1
    

    Tags

    Terminator 35S

    Expression

    Plant

    Promoter

    Lotus japonicus Ubiquitin

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. L309-myc-seipin-CT

    Plasmid

    #64063

    Purpose

    myc-tagged murine seipin (cytosolic C-terminus); lentiviral construct

    Depositor

    Weiping Han

    Article

    Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

    Insert

    bscl2 (Bscl2 Mouse)
    

    Use

    Lentiviral

    Tags

    myc epitope

    Expression

    Mammalian

    Mutation

    Contains Arg326 to Ser443

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA5FRT/TO-p97-RG-mycStrep

    Plasmid

    #31840

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Mutation

    R95G

    Promoter

    CMV

    Available Since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

16. ShaCas9

    Plasmid

    #163794

    Purpose

    Expresses ShaCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUDE714

    Plasmid

    #103021

    Purpose

    expression of a Cpf1 programming crRNA targetting HIS4 (crHIS4-4.S)

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    crHIS4-4.S (HIS4 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330_sgACTA2

    Plasmid

    #63712

    Purpose

    Express sgACTA2 in mammalian cells.

    Depositor

    Stanley Qi

    Article

    Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

    Insert

    ACTA2 (ACTA2 Human, Mouse)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET22b-ecDHFR-N23PP/G51PEKN

    Plasmid

    #118583

    Purpose

    Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Expression

    Bacterial

    Mutation

    N23 mutated to P23, G51 mutated to P51, a Pro res…

    Promoter

    T7

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. 4E2H.210_LUMABS

    Plasmid

    #155209

    Purpose

    Expresses 4E2H.210_LUMABS sensor in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E2H.210_LUMABS
    

    Tags

    6x His Tag

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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