159,352 results

1. pDONR223_NRAS_p.Q61R

   Plasmid

   #81652

   Purpose

   Gateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   NRAS (NRAS Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Q61R

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pITGA4-GFP

   Plasmid

   #81178

   Purpose

   Mammalian expression of human full length ITGA4-EGFP

   Depositor

   Chinten James Lim

   Insert

   ITGA4 (ITGA4 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Contains KLGFFKR motif; A1002L (please see deposi…

   Promoter

   CMV

   Available since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-MS2-HA-HsRoquin1_AE

   Plasmid

   #148611

   Purpose

   Mammalian Expression of HsRoquin1

   Depositor

   Elisa Izaurralde

   Article

   Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

   Insert

   HsRoquin1 (RC3H1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF.DEST51-mScarlet

   Plasmid

   #207960

   Purpose

   Vector for overexpression of the mScarlet fluorescent protein

   Depositor

   Johanna Ivaska

   Article

   Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-fDIO-Cre

   Plasmid

   #121675

   Purpose

   Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

   Depositor

   Esteban Engel, Alexander Nectow

   Article

   Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

   Has Service

   AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

   Insert

   NLS-CRE-HA
   

   Use

   AAV

   Tags

   3xHA and SV40-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-dTAG-Blast

   Plasmid

   #207716

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. 1436 pcDNA3 Flag HA

   Plasmid

   #10792

   Purpose

   pcDNA3 vector with added Flag, HA tags

   Depositor

   William Sellers

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-H3.3(K36M)-3AID-HA-2A-mCherryBSD

   Plasmid

   #225699

   Purpose

   Lentiviral expression of AID degron tagged H3.3(K36M) and mCherry fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   H3.3 (K36M) (H3-3A, H3-3B Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xAID HA and T2A-mCherry

   Expression

   Mammalian

   Mutation

   K36M

   Promoter

   pEF1A

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEMHE-vhhGFP4-hIgG1-Fc

   Plasmid

   #105576

   Purpose

   T7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusion

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   Human IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pARC8-LambdaRedBeta-EcSSB

    Plasmid

    #162572

    Purpose

    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency.

    Depositor

    George Church

    Article

    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

    Inserts

    Lambda Red-Beta
    E. coli SSB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    May 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTD-NTwinStrep_Km

    Plasmid

    #45938

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV5-ActRIIA

    Plasmid

    #11754

    Depositor

    Jeff Wrana

    Article

    Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

    Insert

    Activin receptor IIA (Acvr2a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. YIplac211-SEC7-mCherry2Bx6

    Plasmid

    #105267

    Purpose

    Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-mCherry2Bx6.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Sec7 portion (SEC7 Budding Yeast)
    

    Use

    Tags

    mCherry2Bx6

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRosa26-DEST

    Plasmid

    #21189

    Purpose

    Rosa26 knock-in vector for Cre-regulated cDNA expression and RNAi in transgenic mice

    Depositor

    Peter Hohenstein, Nick Hastie

    Article

    Hohenstein et al Pathogenetics. 2008 . 1(1):3.

    Insert

    GateWay conversion cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. M7 pdnPKA-GFP

    Plasmid

    #16716

    Depositor

    Randall Moon

    Article

    Ungar et al Dev Biol. 1996 Aug 25;178(1):186-91. doi

    Insert

    dnPKA (Prkar1a Mouse)
    

    Use

    Xenopus expression

    Tags

    GFP

    Expression

    Mutation

    Dominant negative. Mutations at cAMP binding site…

    Promoter

    Available since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-TadA8e-PcrA

    Plasmid

    #221228

    Purpose

    Express HACE Editor as a fusion of TadA-8e adenosine deaminase and PcrA helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-PcrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMET7-GAG-EGFP

    Plasmid

    #80605

    Purpose

    Mammalian expression vector that expresses HIV-1 GAG with EGFP fused to it

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBBR1k-GFPuv

    Plasmid

    #106382

    Purpose

    IPTG-inducible trc promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-PGK-Neo-PIP-NLS-mVenus

    Plasmid

    #118619

    Purpose

    Lentiviral expression of the cell cycle marker PIP-NLS-mVenus

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-NLS-mVenus
    

    Use

    Lentiviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDB5322

    Plasmid

    #204832

    Purpose

    An integrating plasmid containing both the inducible enotetSW4 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CMV promoter and enotetSW4 promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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