174,421 results

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pLifeAct-miRFP703

Plasmid

#79993

Purpose

F-Actin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Lifeact

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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ActTS-GR

Plasmid

#79774

Purpose

FRET-based actinin tension sensors

Depositor

Tetsuya Kitaguchi

Article

Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

Insert

ActTS-GR

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-ORF-V2

Plasmid

#73638

Purpose

Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-ORF-V1

Plasmid

#73637

Purpose

Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-rtTA-IN

Plasmid

#60612

Purpose

Backbone for tet-inducible genes

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Type

Empty backbone

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pKHR7

Plasmid

#74624

Purpose

a cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.

Depositor

David Grunwald

Article

Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

Insert

cmlc2::mCherry

Use

Zebrafish expression

Promoter

cmlc2

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-FLCN

Plasmid

#72291

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGFP-Kif5c

Plasmid

#71853

Purpose

mammalian expression of GFP-Kif5c

Depositor

Michelle Peckham

Article

Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95

Insert

Kif5c (Kif5c Rat)

Tags

EGFP

Expression

Mammalian

Mutation

Kif5c starts at AA3

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pclbw-opa1(isoform 1)-myc

Plasmid

#62845

Purpose

Mammalian Expression of myc-tagged Opa1 isoform1

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mmOPA1isoform1-myc (Opa1 Mouse)

Use

Retroviral

Tags

9xMyc

Expression

Mammalian

Promoter

cmv-bactin

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
pEF6-Acly(S455D)

Plasmid

#70768

Purpose

Expresses phosphomimetic mouse ATP-citrate lyase in mammalian cells

Depositor

Kathryn Wellen

Article

Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

Insert

ATP Citrate Lyase (Acly Mouse)

Tags

myc-epitope

Expression

Mammalian

Mutation

S455D

Promoter

EF1alpha

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon

Plasmid

#69906

Purpose

Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ciRS7 (CDR1-AS Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_dSV40-Aurora A-GFP

Plasmid

#67924

Purpose

Expresses Aurora A-GFP in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

GFP-Aurora A (AURKA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

Plasmid

#62044

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

LAP2beta (Tmpo Rat)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-LAC

Plasmid

#64965

Purpose

mini-Tn7 vector with Ptac expression cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pYLsgRNA-OsU6c

Plasmid

#66197

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

OsU6c

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCSF107mT-GATEWAY-3'-3HA

Plasmid

#67616

Purpose

A pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Article

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

three inframe 3' HA tags

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
Myc-E-Syt1

Plasmid

#66833

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt1 (ESYT1 Human)

Tags

Myc

Expression

Mammalian

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pComb3

Plasmid

#63888

Purpose

For phage display of antibody libraries by fusion to modified geneIII.

Depositor

Carlos Barbas

Article

Barbas CF et al Proc Natl Acad Sci U S A. 1991 Sep 15;88

Type

Empty backbone

Use

Phage display

Tags

geneIII

Expression

Bacterial

Promoter

lacZ

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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Str-Ii_neomycin

Plasmid

#65312

Purpose

Cytoplasmic ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Expression

Mammalian

Mutation

Streptavidin in its cytoplasmid part

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pSR660

Plasmid

#65216

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Article

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-8 cap

Use

AAV

Expression

Insect

Promoter

P10 and polyhedrin

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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