We narrowed to 88,480 results for: MAL

1. pCAG-mDrc7-FLAG

   Plasmid

   #176470

   Purpose

   Expression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

   Insert

   dynein regulatory complex subunit 7 (Drc7 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)

   Plasmid

   #178851

   Purpose

   Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   mNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)
   

   Expression

   Mammalian

   Mutation

   Gαq: C9S/C10S/Q209L/L254A

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV Puro dn-cHSF1

   Plasmid

   #134728

   Purpose

   Lentiviral expression vector for constitutively active dominant-negative HSF1

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

   Insert

   dn-cHSF1 (HSF1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 186-202, 379−529

   Promoter

   CMV

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBactin-mOrange2-NEDD1-gTBD

   Plasmid

   #196858

   Purpose

   Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2-N-gTBD (NEDD1 Human)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. RRL.sin.cPPT.SFFV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG536)

   Plasmid

   #139443

   Purpose

   Lentiviral vector to ectopically express human NCOA7 isoform 4 from SFFV promoter

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEX-YFP-STIM1-deltaK

   Plasmid

   #18861

   Depositor

   Tobias Meyer

   Article

   Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

   Insert

   STIM1 (STIM1 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Stop codon inserted at amino acid postion 671 of …

   Available Since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

   Plasmid

   #114087

   Purpose

   U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   entry vector for FnCas12a crRNAs
   

   Expression

   Mammalian

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-mNG-eDHFR(69K6)-G-alpha-q

   Plasmid

   #172106

   Purpose

   Mammalian expression of Gαq fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   mNeonGreen-eDHFR(69K6)-Gαq (GNAQ Human)
   

   Expression

   Mammalian

   Mutation

   Gαq: C9S/C10S/Q209L

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

   Plasmid

   #136660

   Purpose

   Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   ABE7.10
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS and hSt1Cas9

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-LYCHOS (GPR155) WT-HA

    Plasmid

    #199660

    Purpose

    Transient expression of LYCHOS (GPR155) WT

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    GPR155 (GPR155 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-shMBNL1-9155

    Plasmid

    #115460

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shMBNL1-9155 (MBNL1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pODN-mNG-ECT2

    Plasmid

    #183835

    Purpose

    Repair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ECT2 homology arms with mNeonGreen-linker (ECT2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mNeonGreen-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBactin-EGFP-NEDD1-gTBD

    Plasmid

    #196857

    Purpose

    Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    EGFP-N-gTBD (NEDD1 Human)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRE

    Plasmid

    #139088

    Purpose

    Expresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    xCas9 3.7
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    A262T, R324L,S409I, E480K, E543D, M694I, E1219V

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

    Plasmid

    #114089

    Purpose

    CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized FnCas12a
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3_ATP10B

    Plasmid

    #203695

    Purpose

    expresses ATP10B

    Depositor

    Peter Vangheluwe

    Article

    Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

    Insert

    ATP10B (ATP10B Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-UBC-S2-RAB_EKARev

    Plasmid

    #160726

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S2-RAB_EKARev
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. LoxP1-His-H2B-Cherry-2A (SO290)

    Plasmid

    #99616

    Purpose

    To clone gene of interest downstream of LoxP1-His-H2B-Cherry-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    6xHis-H2B-Cherry
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139091

    Purpose

    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dSpCas9-NG-VPR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-UBC-S3-RAB_EKARev

    Plasmid

    #160727

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S3-RAB_EKARev
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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