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pSFFV_mNG3K(1-10)

Plasmid

#157993

Purpose

Lentiviral vector of constitutive expression of mNeonGreen3K(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

pSFFV_mNG3K(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

G27D, D42E, M128I, K153M, T170I

Promoter

SFFV

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA

Plasmid

#128034

Purpose

Mammalian expression vector with N terminal HA tag

Depositor

Oskar Laur

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pv6_MCPH1_BRCT W706RW815R

Plasmid

#250355

Purpose

Expresses mutant tandem BRCT domain from MCPH1 fused to eGFP. Serves as control.

Depositor

Tuncay Baubec

Article

da Silva et al Nat Commun. 2025 Nov 20;16(1):10127. doi

Insert

MCPH1 tandem BRCT

Tags

NLS, eGFP and biotintag, TEV cleavage

Expression

Mammalian

Mutation

W706R and W815R

Promoter

CAGGS

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-h-cGAS

Plasmid

#127161

Purpose

expression of human cGAS protein

Depositor

Thomas Tuschl

Article

Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

Insert

MB21D1 (CGAS Human)

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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DHFR-SpCas9-DHFR

Plasmid

#85447

Purpose

Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-SpCas9-DHFR

Use

AAV

Tags

Destabilized domain (C-terminus version) of E.col…

Expression

Mammalian

Promoter

Cbh

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-BFP-sesRNA-tTA2-WPRE-pA

Plasmid

#192070

Purpose

Expression of BFP, sesRNA in mammalian cells, with tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for tdTom

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-SB2

Plasmid

#228868

Purpose

PE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequence

Depositor

Sangsu Bae

Article

Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

Insert

PE7-2A-MLH1-SB-NLS-2A-MLH1-SB

Use

CRISPR

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRGEB32

Plasmid

#63142

Purpose

Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.

Depositor

Yinong Yang

Article

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Rice snoRNA U3 promoter for gRNA/PTG expression a…

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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3xAP1pGL3 (3xAP-1 in pGL3-basic)

Plasmid

#40342

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

3xAP-1

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains three canonical AP-1 binding sites (TGAC…

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pDisCoTune

Plasmid

#173713

Purpose

Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

Depositor

Morten Norholm

Article

Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2

Inserts

ERV1 (ERV1 Budding Yeast)
PDI

Expression

Bacterial

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-hMLH1dn

Plasmid

#222997

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-hMLH1dn

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Deletion of residues 754-756 in MLH1 as described…

Promoter

CMV

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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SP-dCas9-VPR

Plasmid

#63798

Purpose

SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

SP-dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pKC105_pLenti_TetON_PABPC1_INT

Plasmid

#242110

Purpose

Tet- or Dox-inducible expression of PABPC1 fused to INT (human PARP4 fragment)

Depositor

Fei Chen

Article

Chao et al Science. 2026 Jan 15:eadz9353. doi: 10.1

Insert

PAB4L, PARP4 fragment (PABPC1 Human)

Use

Lentiviral

Mutation

PABPC1 aa 1-370, PARP4 aa 1562-1724

Promoter

TRE3G

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Cytoplasmic PancACe

Plasmid

#215708

Purpose

Expresses acetyl-CoA biosensor in the cytoplasm

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and cytoplasmic localization sequence

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRRLSIN.cPPT.PGK-GFP.WPRE

Plasmid

#12252

Purpose

3rd generation lentiviral backbone

Depositor

Didier Trono

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2007

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG2(11)1-10

Plasmid

#82610

Purpose

Lentiviral vector of constitutive expression of mNeonGreen2(1-10)

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNeonGreen2(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

K128M, S142T, R150M, G172V and K213M

Promoter

SFFV

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19

Plasmid

#50005

Purpose

pUC cloning vector

Depositor

Joachim Messing

Article

Norrander et al Gene. 1983 Dec;26(1):101-6.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cloning vector

Promoter

lac

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pML104

Plasmid

#67638

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

Depositor

John Wyrick

Article

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Inserts

Cas9
single guide RNA expression cassette

Use

CRISPR

Expression

Yeast

Promoter

pSNR52 and pTDH3

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VPR-Puro

Plasmid

#99373

Purpose

3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

(Sp)dCas9-VPR-P2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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Mitochondrial PancACe

Plasmid

#215710

Purpose

Expresses acetyl-CoA biosensor in the mitochondria

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and mitochondrial localization sequence…

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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