164,577 results

1. pYX122-mtGFP

   Plasmid

   #45048

   Depositor

   Benedikt Westermann

   Article

   Westermann et al Yeast. 2000 Nov;16(15):1421-7.

   Insert

   mtGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TPI

   Available Since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pPICZ-pre-pro-alphaf(I)-E2-Crimson

   Plasmid

   #117660

   Purpose

   Test intracellular localisation of E2-Crimson and study the secretion efficiency

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-pro-alphaf(I)-E2-Crimson
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   This plasmid encodes the fluorescent protein E2-C…

   Promoter

   pAOX1

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHM106Env

   Plasmid

   #139024

   Purpose

   Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

   Depositor

   Anjali Joshi

   Article

   Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

   Insert

   HIV-1 Envelope Glycoprotein (env HIV-1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. BM00369

   Plasmid

   #155338

   Purpose

   Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling GUS. It constitutively expresses dsRed and a plant selection cassette.

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-dsRed-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-GUS-POpto_LB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-MinE-His

   Plasmid

   #133623

   Purpose

   Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

   Insert

   MinE-His (minE E. coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWZL Neo Myr Flag ADRBK1

   Plasmid

   #20418

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   ADRBK1 (GRK2 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA-ET

   Plasmid

   #72542

   Purpose

   expresses FLAG tagged human BRD4 ET domain (608-699 aa)

   Depositor

   Christopher Vakoc

   Article

   Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

   Insert

   BRD4 ET (BRD4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_EDNRB_WT

   Plasmid

   #81881

   Purpose

   Gateway Donor vector containing EDNRB , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   EDNRB (EDNRB Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-hSyn-SpdCas9-W3SL

   Plasmid

   #210708

   Purpose

   This Plasmid express hSyn promoter driven SpdCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   S. Pyogenes dCas9
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   hSyn

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. MAC_N_ZIC3

    Plasmid

    #167809

    Purpose

    Expresses ZIC3 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    ZIC3 (ZIC3 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. YL-CashGem-BAT1-URA3-LINEAR

    Plasmid

    #174837

    Purpose

    pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GPDp

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUW-TetO-Klf5

    Plasmid

    #128830

    Purpose

    dox-dependent expression of mouse Klf5

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Klf5 (Klf5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSilencer-Opa1 KO

    Plasmid

    #191949

    Purpose

    Expression of a siRNA that targets Opa1

    Depositor

    Luca Scorrano

    Insert

    Opa1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-puro/Flag PD-L1 N35/3NQ

    Plasmid

    #121455

    Purpose

    expression of human PD-L1 mutant in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N192Q, N200Q and N219Q

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRB051_LEU2

    Plasmid

    #196993

    Purpose

    Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

    Depositor

    Matthew Maurano

    Article

    Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.

    Type

    Empty backbone

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJEP203-Lenti-TRE3G-GFPGluN2B-WPRE-pA

    Plasmid

    #164787

    Purpose

    pLenti vector backbone designed to express GFP-GluN2B from a TRE3G promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    GluN2B
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    1.3αCaMKII

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PK187

    Plasmid

    #44623

    Depositor

    Robert Oshima

    Article

    Oshima et al Differentiation. 1986;33(1):61-8.

    Insert

    K18 (KRT18 Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX6P1-human LIC1 C-terminal half

    Plasmid

    #74599

    Purpose

    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    

    Use

    Tags

    N-terminal GST and Strep II

    Expression

    Bacterial

    Mutation

    amino acids 389-523 of LIC1

    Promoter

    T7

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTYB2-eIF1A

    Plasmid

    #37218

    Depositor

    Jon Lorsch

    Article

    Acker et al Methods Enzymol. 2007;430:111-45.

    Insert

    eIF1A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

20. p404GALS

    Plasmid

    #17435

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    GALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    GALS promoter is between SacI and XbaI restrictio…

    Promoter

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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