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Showing: 2651 - 2700 of 22679 results

  5. His6-pAG-RTase

    Plasmid
    #214932
    Purpose
    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems
    Depositor
    Chuan He
    Article
    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s
    Insert
    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…
    Promoter
    T7 promoter
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    214932-plasmid-map-sequence-id-426479

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  16. pcDNA3-FRB-VMAc-CSarcin

    Plasmid
    #70223
    Purpose
    Expresses VMA intein with C-terminal portion of alpha-sarcin
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    alpha Sarcin (C-terminal portion)
    Use
    Tags
    FKBP-rapamycin binding (FRB) domain of the mammal…
    Expression
    Mammalian
    Mutation
    Changed Asp 28 to Cys, deleted amino acids 1-27
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  28. pQCXIP GFP-MOSPD2 RD/LD

    Plasmid
    #186468
    Purpose
    Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to EGFP in mammalian cells
    Depositor
    Catherine Tomasetto
    Article
    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017
    Insert
    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    R404D/L406D (mutant unable to bind FFAT motifs)
    Promoter
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186468-plasmid-map-sequence-id-366060

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  31. AKARdual

    Plasmid
    #99869
    Purpose
    Measure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrate
    Depositor
    Marc Tramier
    Article
    Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10
    Insert
    AKAR4
    Use
    Tags
    LSSmOrange and mKate2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    99869-plasmid-map-sequence-id-192827

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  36. pPD47_52

    Plasmid
    #1446
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    Sp6p, T7p, OriLam>>, OriLam<<, KanRN,…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1446-plasmid-map-sequence-id-57

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  38. pcDNA3.1_mGFP-FGFR3-K650Q

    Plasmid
    #191756
    Purpose
    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650Q mutation (associated with HCH; target mutation: c.1948A>C; silent mutation added on purpose: c.1938C>T)
    Depositor
    Irene Tiemann-Boege
    Article
    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi
    Insert
    mGFP-FGFR3IIIc
    Use
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    c.1948A>C (p.K650Q) + silent mutation c.1938C&…
    Promoter
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191756-plasmid-map-sequence-id-382285

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  40. pCMK188

    Plasmid
    #226659
    Purpose
    pCB2.4 - pBR322 shuttle vector that replicates in E. coli and Synechococcus sp. PCC 7002, contains HIP1 site
    Depositor
    David Nielsen
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    226659-plasmid-map-sequence-id-455439

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  43. pcDNA-mC/EBPb

    Plasmid
    #49198
    Purpose
    expression of mouse C/EBPbeta in mammalian cells
    Depositor
    Jed Friedman
    Insert
    C/EBPbeta (Cebpb Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    49198-plasmid-map-sequence-id-265902

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  45. pCMK74

    Plasmid
    #226657
    Purpose
    Broad host range WVO1 vector that replicates in Synechococcus sp. PCC 7002, KanR, contains HIP1 site
    Depositor
    David Nielsen
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    226657-plasmid-map-sequence-id-455440

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  50. pAdTrack-mC/EBPbeta

    Plasmid
    #49196
    Purpose
    adenoviral shuttle vector with mouse C/EBPbeta
    Depositor
    Jed Friedman
    Insert
    C/EBPbeta LAP fragment (Cebpb Mouse)
    Use
    Adenoviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 2651 - 2700 of 22679 results