165,744 results

1. pAAVS1-TRE-MA4-GFP

   Plasmid

   #114380

   Purpose

   Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.

   Depositor

   Yuet Wai Kan

   Article

   Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

   Insert

   MLL-AF4 fusion gene (AFF1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIBN(deltaNLS)-pmGFP

   Plasmid

   #26867

   Purpose

   Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   NLS sequences mutated

   Promoter

   CMV

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR1K18ms

   Plasmid

   #72644

   Purpose

   Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

   Depositor

   Brian Kvitko

   Article

   Mijatovic et al Phytopathology. 2021 Dec;111(12):2176-21

   Type

   Empty backbone

   Use

   Sucrose counter-selection allelic exchange vector

   Available Since

   March 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLEX-MCS PGK promoter

   Plasmid

   #128044

   Purpose

   Lentiviral vector with PGK promoter

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Mito-B

   Plasmid

   #209864

   Purpose

   Dimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP B
   

   Tags

   Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. HPF1-c004

   Plasmid

   #216874

   Purpose

   Bacterial expression vector for E. coli producing full length HPF1 protein with N-terminal YFP fusion

   Depositor

   Lari Lehtiö

   Article

   Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

   Insert

   Full length HPF1 (HPF1 Human)
   

   Expression

   Bacterial

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-HDR-mEGFP-camk2a

   Plasmid

   #104589

   Purpose

   AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse camk2a

   Depositor

   Ryohei Yasuda

   Article

   Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Promoter

   None

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRE3G-Bi-gePSI-pCamk2a-AcGFP

   Plasmid

   #133732

   Purpose

   Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.

   Depositor

   Erin Schuman

   Article

   Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Inserts

   gePSI-β-chain
   gePSI-α-chain
   AcGFP1
   

   Use

   AAV

   Tags

   murine ornithine decarboxylase - degron (ODC36)

   Expression

   Mammalian

   Promoter

   pCamk2a and pTRE3G-Bi

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AIMTOR T757

   Plasmid

   #140828

   Purpose

   AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells

   Depositor

   Anne Bonnieu

   Article

   Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

   Insert

   AIMTOR T757 (ULK1 Human)
   

   Tags

   NES Nuclear export signal

   Expression

   Mammalian

   Mutation

   The insert comprises only a small sequence of hum…

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJR85

    Plasmid

    #140095

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs1 in stem loop 2 of the constant region
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-SEAP

    Plasmid

    #24595

    Depositor

    Alan Cochrane

    Insert

    SEAP (ALPP Human)
    

    Expression

    Mammalian

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti6MQBI163 SGLT2

    Plasmid

    #194147

    Purpose

    Expresses SGLT2 protein in mammalian cells

    Depositor

    Jun Liu

    Insert

    SLC5A2 (SLC5A2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. p206-Switch-ON

    Plasmid

    #217885

    Purpose

    Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Retroviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST14-SpyCatcher002

    Plasmid

    #102827

    Purpose

    Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyCatcher002
    

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. WIW_Scaffold2

    Plasmid

    #205574

    Purpose

    Nanobody that inhibits seeding of tau fibrils

    Depositor

    David Eisenberg

    Article

    Abskharon et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

    Insert

    WIW_Scaffold2
    

    Tags

    6X His

    Expression

    Bacterial

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDF-5xT7-TtCsm

    Plasmid

    #128572

    Purpose

    For expression of T. thermophilus Csm in E. coli (Csm1-5)

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

    Inserts

    Csm1
    Csm2, Csm3, Csm4, Csm5
    

    Tags

    HRV 3C protease cleavage site and 10xHis tag on C…

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli and Cod…

    Promoter

    T7 promoter, lac operator

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3/td5StayGold(c4)=GianCreg

    Plasmid

    #212022

    Purpose

    For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GianCreg (GOLGB1 Human)
    

    Tags

    td5StayGold(c4)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-PIG-miR-29b-1/miR-29a

    Plasmid

    #64231

    Purpose

    Expresses miR-29b-1 and miR-29a in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

    Insert

    miR-29b-1 and miR-29a (MIR29A, MIR29B1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. opto-Rac1

    Plasmid

    #135396

    Purpose

    Expresses opto-Rac1 (BcLOV4-GGGSx2-WT_Rac1-GGGSx2-mCherry in mammalian cells in the pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al Photochem Photobiol Sci. 2020 Mar 1;19(3

    Insert

    opto-Rac1
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. TALEN-CCR5L18-CtermQ3

    Plasmid

    #51440

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) Q3 C-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Mutation

    K788Q, R792Q and R801Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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