Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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pRSF_his6_sumo_SARS_CoV2_NSP14

Plasmid

#213518

Purpose

Expresses NSP14 of SARS-CoV-2

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

N-terminal His6-sumo

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+HIF1a-AS1_deltaExon1 (nucleotides 26-78)

Plasmid

#194175

Purpose

Expresses lncRNA HIF1a-AS1 mutant

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

HIF1a-AS1 (HIF1A-AS1 Human)

Expression

Mammalian

Mutation

Within Exon1, nucleotides 26-78 were removed

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta55-118-10xHis

Plasmid

#194180

Purpose

Expresses MPP8-delta55-118_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta55-118 (chromo domain deletion)

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-Cry2-mCherry- YAP-N-IDR

Plasmid

#166446

Purpose

Expresses fusion of CRY2PHR with mCherry and YAP-N-IDR

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

Cry2, mCherry, YAP-N-IDR(1-164) (YAP1, CRY2 Human)

Use

Lentiviral

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4-eIF3B MBE2 mutant

Plasmid

#133309

Purpose

Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2

Depositor

Josep Domingo-Domenech

Article

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter MBE2 mutant (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Mutation

gccacatgcacc changed to gcAaAaAAcacc

Promoter

eIF3B

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_omicron_BA.1

Plasmid

#180375

Purpose

Pseudovirus plasmid expressing SARS-CoV-2 omicron BA.1 strain spike protein

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (omicron BA.1 variant) (S SARS-CoV-2)

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

c terminal D18

Promoter

CMV

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_omicron BA.2

Plasmid

#183700

Purpose

expressing SARS-CoV-2 BA.2 spike protein for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (BA.2) (S SARS-CoV-2)

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

c-terminal 18 aa deletion

Promoter

CMV

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCW-codon optimized catalytically inactive PHGDH

Plasmid

#154903

Purpose

Expresses codon optimized catalytically inactive PHGDH in mammalian cells

Depositor

Michael Pacold

Article

Ngo et al Cancer Discov. 2020 Sep;10(9):1352-1373.

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Three mutations: D175N, R236K, H283A

Promoter

TRE

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_BA.4/5

Plasmid

#186810

Purpose

pseudovirus plasmid expressing BA.4 and BA.5 spike protein

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

43740568 (S SARS-CoV-2)

Expression

Mammalian

Mutation

HY69-70del, L452R, F486V, Q493R, on the basis of …

Promoter

CMV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-Beta

Plasmid

#177962

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G) (S )

Expression

Mammalian

Mutation

K417N, E484K, N501Y, D614G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_omicron_BA.2.75

Plasmid

#190674

Purpose

expressing SARS-CoV-2 BA.2.75 spike protein for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Cell Host Microbe. 2022 Nov 4. pii: S193

Insert

Spike (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147E, W152R, F157L, I210V, G257S, D339H, G446S, …

Promoter

CMV

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-Epsilon

Plasmid

#177964

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G) (S )

Expression

Mammalian

Mutation

S13I, W152C, L452R, D614G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-K417N

Plasmid

#177969

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, K417N)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, K417N) (S )

Expression

Mammalian

Mutation

D614G, K417N

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-N439K

Plasmid

#177970

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N439K)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, N439K) (S )

Expression

Mammalian

Mutation

D614G, N439K

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-L452R

Plasmid

#177971

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, L452R)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, L452R) (S )

Expression

Mammalian

Mutation

D614G, L452R

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-S477N

Plasmid

#177972

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S477N)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, S477N) (S )

Expression

Mammalian

Mutation

D614G, S477N

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-N501Y

Plasmid

#177975

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N501Y)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, N501Y) (S )

Expression

Mammalian

Mutation

D614G, N501Y

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-S13I

Plasmid

#177965

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S13I)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, S13I) (S )

Expression

Mammalian

Mutation

D614G, S13I

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-T20N

Plasmid

#177966

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, T20N)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, T20N) (S )

Expression

Mammalian

Mutation

D614G, T20N

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-P26S

Plasmid

#177967

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, P26S)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, P26S) (S )

Expression

Mammalian

Mutation

D614G, P26S

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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