We narrowed to 30,611 results for: TRO

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pAAV-hSyn-SIO-PdCO-mScarlet-WPRE

Plasmid

#198509

Purpose

Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

hSyn1

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR

Plasmid

#197265

Purpose

Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

Type

Empty backbone

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Expression

Mammalian

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLJD-KLF4-ER

Plasmid

#34596

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Retroviral

Tags

Myc epitope tag and estrogen receptor fusion

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pEX-CFP-hLC3ΔG

Plasmid

#24986

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a mutant human MAP1-LC3B fused with ECFP (MAP1LC3B Human)

Tags

ECFP

Expression

Mammalian

Mutation

deleted Gly at 121

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
AAVS1-Puro CAG PSMA nanobody T-CAR

Plasmid

#171962

Purpose

Donor plasmid for PSMA nanobody T-CAR expression in human cells

Depositor

Xiaoping Bao

Article

Harris et al J Immunol Regen Med. 2023 May;20:100074.

Insert

Synthetic anti-PSMA nanobody CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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AAV hSYN DIO mGluR1-myc-FLAG

Plasmid

#166227

Purpose

AAV expressing cre-dependent mGluR1-myc-FLAG

Depositor

Jason Aoto

Article

Cai et al Neuron. 2021 Apr 7;109(7):1137-1149.e5.

Insert

metabotropic glutamate receptor 1 (Grm1 Mouse)

Use

AAV and Cre/Lox

Tags

Myc and FLAG

Expression

Mammalian

Promoter

human Synapsin

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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DHC1-AID-Clover donor (Neo)

Plasmid

#158621

Purpose

Donor plasmid for tagging DHC1 with AID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR; Tagging donor

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-p62(W340A/ΔUBD)

Plasmid

#119688

Purpose

For mammalian expression of LIR mutant p62 without its UBD

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

p62 (SQSTM1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

W340A & UBD deletion (1-385)

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pQCXIX-Myc-hTIM3

Plasmid

#110893

Purpose

Expression of Myc-tagged hTIM3 at the cell surface

Depositor

Hyeryun Choe

Article

Jemielity et al PLoS Pathog. 2013 Mar;9(3):e1003232. doi

Insert

Hepatitis A virus Cellular Receptor 2 (HAVCR2) (HAVCR2 Human)

Use

Retroviral

Tags

Myc

Promoter

CMV

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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CRY2-INPP5E

Plasmid

#79562

Purpose

expression of CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

INPP5E (INPP5E Human)

Tags

CRY2 (photolyase homology region domain of Arabid…

Expression

Mammalian

Mutation

C-terminal region, aa 214_644, C641A mutation to …

Promoter

CMV

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only
pMSCV40-EF1a-hBCL10-SO

Plasmid

#75256

Purpose

retroviral expression vector for expression of StrepOne-Tagged human BCL10

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

Bcl10 (BCL10 Human)

Use

Retroviral

Tags

StrepOne Tag

Expression

Mammalian

Promoter

pMSCV-LTRs, EF1a

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pdCas9-M-3A2

Plasmid

#73435

Purpose

CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

Repressor 3A2 (orthogonal T7-lac repressor)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Constitutive wild-type S. pyogenes promoter

Available Since

March 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO GFP MPS1 K-R

Plasmid

#59819

Purpose

Allows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Tighe et al J Cell Biol. 2008 Jun 16;181(6):893-901.

Insert

Mps1 (TTK Human)

Tags

EmGFP

Expression

Mammalian

Mutation

KD, catalytically inactive, D664A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(30Q)

Plasmid

#21753

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 32 amino acids (30 Q…

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only
pMXs-hu-c-Myc-dN1

Plasmid

#50773

Purpose

retroviral expression of human c-Myc dN1 fragment

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

c-Myc -dN1 (MYC Human)

Use

Retroviral

Expression

Mammalian

Mutation

N-terminal deletion, contains aa 14-439

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pCLXSN-Tax

Plasmid

#44038

Depositor

Shao-Cong Sun

Article

Rivera-Walsh et al J Biol Chem. 2001 Nov 2;276(44):40385-8.

Insert

5'UTR-Tax-3'UTR (CNTN2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only
pMXs-IP-EGFP-ULK2(K39T)

Plasmid

#38206

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

Unc-51 like kinase 2 (Ulk2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed Lysine 39 to Threonine

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO HIS DNAJB6a

Plasmid

#19556

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB6a (DNAJB6 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pLPC FLAG hRap1 delta Br delta M

Plasmid

#13256

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Br delta M (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Mutation

contains hRap1 aa2-44 + 184-399 and the point mut…

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pLPC FLAG hRap1 delta Myb

Plasmid

#13253

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Myb (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Mutation

contains hRap1 aa2-131 + 184-399 and the point mu…

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only