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We narrowed to 126,154 results for: CIL

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Showing: 10901 - 10920 of 126154 results

  7. Ezh2[SET]-dCas9

    Plasmid
    #100087
    Purpose
    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    Ezh2[SET] (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Ezh2[SET] includes aa 482-746
    Promoter
    CMV
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100087-plasmid-map-sequence-id-197556

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  13. pX330-BsaIx2-DD-Cas9

    Plasmid
    #75380
    Purpose
    Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1
    Depositor
    Michele Calos
    Article
    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv
    Insert
    DD-SpCas9
    Use
    CRISPR
    Tags
    FKBP12 L106P DD
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    75380-plasmid-map-sequence-id-192102

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  16. PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins

    Plasmid
    #78099
    Purpose
    Nuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).
    Depositor
    Michael Elowitz
    Article
    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do
    Insert
    Histone H2B - Citrine (H2BC21 Synthetic, Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    5xTetO pEF alpha
    Available Since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid
    #197511
    Purpose
    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and BPNLS-NpuC(intein)
    Mutation
    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…
    Promoter
    Cbh
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197511-plasmid-map-sequence-id-386647

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Showing: 10901 - 10920 of 126154 results