166,169 results

1. pGW-GFP-GOI-TurboID

   Plasmid

   #209382

   Purpose

   Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pADH118

   Plasmid

   #90983

   Purpose

   NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. albicans LEU2 2 of 2
   

   Use

   CRISPR

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pADH137

   Plasmid

   #90986

   Purpose

   NAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH118-series gRNA expression constructs.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Inserts

   C. albicans LEU2 1 of 2
   pENO1
   Cas9
   NAT 1 of 2
   

   Use

   CRISPR

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPI226

   Plasmid

   #113228

   Purpose

   safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-mCherry

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Insert

   LifeAct
   

   Use

   Safe haven knock-in plasmid for the act5 locus, l…

   Tags

   mCherry

   Promoter

   act5

   Available Since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pADH119

   Plasmid

   #90985

   Purpose

   NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   gRNA conserved, C. albicans LEU2 2 of 2
   

   Use

   CRISPR

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTRbasEGFP

   Plasmid

   #22453

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Insert

   EGFP (eGFP )
   

   Use

   Entry vector

   Available Since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

   Plasmid

   #209086

   Purpose

   Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   BirA
   

   Tags

   AviTag

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   TRE4G and hSyn

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBW2602_pCAG-PV1-iCre-N256-L1-FRB-NLS-BGHpA

   Plasmid

   #108735

   Purpose

   Expresses N-terminus to aa256 of Cre recombinase fused to FRB CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment N-terminus to aa256
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FRB,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. tCRMod-GGSGGS5

   Plasmid

   #111760

   Purpose

   Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-GGSGGS5-mRuby2 fusion
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPI227

    Plasmid

    #113231

    Purpose

    safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-GFP

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    LifeAct
    

    Use

    Safe haven knock-in plasmid for the act5 locus,li…

    Tags

    GFP

    Promoter

    act5

    Available Since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_TBK1_WT

    Plasmid

    #82285

    Purpose

    Gateway Donor vector containing TBK1, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Gateway entry vector

    Mutation

    N388D; K570Q

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. MAC-CTSL

    Plasmid

    #172414

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    Procathepsin L (Cathepsin L1) (CTSL Human)
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Nov. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. HaloTag-G protein gamma-1

    Plasmid

    #67038

    Purpose

    G protein gamma-1 tagged with HaloTag on amino terminus

    Depositor

    Catherine Berlot

    Insert

    HaloTag-G protein gamma-1 (GNGT1 Bovine)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX307-CTSB-G418

    Plasmid

    #158465

    Purpose

    Constitutive lentiviral expression of human CTSB.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    CTSB (CTSB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-ConVERGD-ArchT(eGFP)-WPRE

    Plasmid

    #218760

    Purpose

    Provides AND intersectional (Cre+Flp) expression of ArchT-eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    ArchT
    

    Use

    AAV

    Tags

    eGFP

    Promoter

    hSyn

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3796 pGEX-16E2 (wt)

    Plasmid

    #24123

    Depositor

    Peter Howley

    Article

    Schweiger et al J Virol. 2006 May . 80(9):4276-85.

    Insert

    HPV16 E2 WT
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    None

    Available Since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

17. TMEM216-mCherry

    Plasmid

    #41633

    Depositor

    Joseph Gleeson

    Article

    Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

    Insert

    Transmembrane Protein 216 (TMEM216 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pUAS-kkv::NG

    Plasmid

    #138953

    Purpose

    Used to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila using Gal4

    Depositor

    Paul Adler

    Article

    Adler et al bioRxiv 2020

    Insert

    kkv (NEWENTRY Fly)
    

    Use

    Uas transgene vector

    Tags

    Neon Green fused to C terminus of Kkv

    Expression

    Insect

    Available Since

    Nov. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWalium-ChtVis-Tomato

    Plasmid

    #67756

    Purpose

    Expresses a fluorescent chitin reporter under Gal4 control in Drosophila.

    Depositor

    Paul Adler

    Article

    Sobala et al Development. 2015 Nov 15;142(22):3974-81

    Insert

    dyl - chitin binding domain - tdTomato - dyl
    

    Tags

    td-tomato

    Expression

    Insect

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-ELMOD1-myc-His6

    Plasmid

    #201900

    Purpose

    Used for expression of human protein ELMOD1 in mammalian cells

    Depositor

    Richard Kahn

    Insert

    ELMOD1 (ELMOD1 Human)
    

    Tags

    myc, 6xHis

    Expression

    Mammalian

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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