Addgene

We narrowed to 22,198 results for: Sele

Showing: 1621 - 1650 of 22198 results

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pSF463

Plasmid

#167671

Purpose

Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-SREBP-1c

Plasmid

#26802

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

SREBP-1c (SREBF1 Human)

Use

Tags

2XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pTarget

Plasmid

#186452

Purpose

used as source for acquisition of new spacers

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSF280

Plasmid

#167675

Purpose

Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSF279

Plasmid

#167674

Purpose

Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Kan

Plasmid

#232099

Purpose

Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

Depositor

Jeffrey Lewis

Article

Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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His-PIMT

Plasmid

#34852

Depositor

Bruce Downie

Article

Dinkins et al Plant J. 2008 Jul;55(1):1-13.

Insert

PIMT (PCMT1 Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-2xFLAG-SREBP-1a

Plasmid

#26801

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

SREBP-1a (SREBF1 Human)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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p11-LacY-wtx1

Plasmid

#69056

Purpose

The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

Depositor

Huimin Zhao

Article

Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

Inserts

ccdB (ccdB )
lacY (lacY )

Use

Tags

A177C

Expression

Mutation

Promoter

pBAD and pLac

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pBad-mCherry-XL (R125K)

Plasmid

#188054

Purpose

Expresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteria

Depositor

Ralph Jimenez

Article

Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

Insert

mCherry-XL (R125K)

Use

Tags

6xHis

Expression

Bacterial

Mutation

differs from mCherry by R125K, W143S, I161V, Q163…

Promoter

araBAD

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMS79 (eft-3p::Cas9 + sgRNA)

Plasmid

#154839

Purpose

Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

sgRNA

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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HA-GFP

Plasmid

#137763

Purpose

Control plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GFP

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB109

Plasmid

#68563

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

3xFLAG

Expression

Plant

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast

Plasmid

#125133

Purpose

Lentiviral empty backbone control vector with CMV promoter and Blasticidin resistance gene

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-TAX1BP1

Plasmid

#175761

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-TAX1BP1

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-ZFN-AAVS1

Plasmid

#89707

Purpose

Mammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locus

Depositor

Dmitriy Mazurov

Article

Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

Insert

ZFN-AAVS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDD282

Plasmid

#66823

Purpose

GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

GFP-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized GFP

Expression

Worm

Mutation

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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PL-SIN-Oct4-EGFP

Plasmid

#21319

Purpose

Lentiviral vector, expresses EGFP under Oct3/4 promoter

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Oct-4 promoter region

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1

Plasmid

#175766

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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PL-SIN-EOS-S(4+)-EGFP

Plasmid

#21317

Purpose

Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EOS-S(4+) promoter region

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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PL-SIN-Nanog-EGFP

Plasmid

#21321

Purpose

Lentiviral vector, expresses EGFP under Nanog promoter

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Nanog promoter region

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pRSET YBX-1

Plasmid

#13035

Depositor

Peter Newburger

Article

Shen et al J Biol Chem. 1998 Mar 6. 273(10):5443-6.

Insert

YBX1 (YBX1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-CBA-DO(FAS)-GCaMP6s

Plasmid

#110135

Purpose

Cre-off expression of GCaMP6s

Depositor

Bernardo Sabatini

Article

Markowitz et al Cell. 2018 May 10. pii: S0092-8674(18)30

Insert

GCaMP6s

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6c

Plasmid

#215821

Purpose

Express CBE6c (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6c-SpCas9-2xUGI (cas9 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pTrex-Luc-Neo

Plasmid

#48336

Purpose

Trasgenically expresses firefly luciferase in T. cruzi with neomycin selection

Depositor

Rick Tarleton

Article

Sanchez-Valdez et al Elife. 2018 Mar 26;7. pii: 34039. doi: 1

Insert

firely luciferase

Use

Trypanasoma cruzi expression

Tags

Expression

Mutation

Promoter

T. cruzi rRNA

Available since

July 20, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMpGWB300

Plasmid

#68628

Purpose

MCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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mOtop3_pGEMHE

Plasmid

#114676

Purpose

This construct was used to express mouse Otop3 in Xenopus laevis oocytes

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 3 (Otop3 Mouse)

Use

For generating the mrna, which can highly express…

Tags

Expression

Mutation

Promoter

T7

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4 huZAP(S)

Plasmid

#45906

Depositor

Harmit Malik

Article

Kerns et al PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1

Insert

ZAP(S) isoform (ZC3HAV1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

M202T, L388F

Promoter

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_miniSOG2 T2A H2B-EGFP

Plasmid

#87410

Purpose

Mammalian expression vector encoding Histone 2B GFP fusion and miniSOG2

Depositor

Xiaokun Shu

Article

Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

Insert

mini singlet oxygen generator 2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pQTEV-SCP2

Plasmid

#31336

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

sterol carrier protein 2 (SCP2 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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