We narrowed to 4,114 results for: SPL

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pYAL_HLA-A2_MART1

Plasmid

#171177

Purpose

Displays MART-1 peptide in HLA-A*02 as a single-chain trimer

Depositor

Michael Birnbaum

Article

Huisman et al Methods Mol Biol. 2022;2491:263-291. doi

Insert

MART1_HLA-A2*01 single-chain trimer fusion

Expression

Bacterial and Yeast

Promoter

GAL1

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-pMagHigh-FlpC

Plasmid

#180586

Purpose

PA-Flp

Depositor

Won Do Heo

Article

Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

Insert

Split FlpO

Use

AAV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGLOW79

Plasmid

#177339

Purpose

C. elegans mNeonGreen co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-3::mNeonGreen

Tags

mNeonGreen

Expression

Worm

Promoter

myo-3

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pdCas9+sgRNA expression vector precursor

Plasmid

#171798

Purpose

PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression

Depositor

Stephen Elledge

Article

Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

pLtetO

Available Since

Aug. 16, 2021

Availability

Academic Institutions and Nonprofits only

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V180 pLP Triple-Flag SD - Acceptor

Plasmid

#11707

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

3xFLAG

Expression

Mammalian

Available Since

June 22, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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aP2-GFPtpz

Plasmid

#114211

Purpose

aP2/Fabp4 promoter driving GFPtopaz for labeling differentiated adipocytes and macrophage

Depositor

David Rowe

Article

Case et al Bone. 2013 Jan;52(1):454-64. doi: 10.101

Insert

aP2 promoter + pOB4 + eGFPtopaz (Fabp4 Synthetic, Aequorea victoria, Mouse)

Tags

None

Expression

Mammalian

Promoter

aP2/Fabp4

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-GFP-SF3B6-FLAG

Plasmid

#86870

Purpose

To express SF3B6 with a GFP and FLAG tags at N- and C-terminus, respectively.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

SF3B6 (SF3B6 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalA (short) wt

Plasmid

#19723

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

NOTE: short splice variant Long Sp. Variant: MV…

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
p10xUAS-luc2

Plasmid

#173006

Purpose

firefly luciferase reporter vector with 10x clustered UAS binding sites

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

10xUAS-luc2

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nV5-AMOTL1

Plasmid

#172998

Purpose

constitutive expression of V5-tagged AMOTL1

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

V5-AMOTL1 (AMOTL1 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC051-HisMS2_PLP_pac_LC

Plasmid

#128234

Purpose

Lower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

Depositor

Paul Freemont

Article

de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

Inserts

Maturation Protein
Coat Protein Dimer

Expression

Bacterial

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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(271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706)

Plasmid

#168102

Purpose

HA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

HA-nEPEA-(EAAAK)3-OGA(544-706)

Tags

HA-tag

Expression

Mammalian

Mutation

OGA truncated to contain residues 544-706

Promoter

T7/CMV

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-IKZF1-V1-Q146H-FLAG-IRES-GFP

Plasmid

#69048

Purpose

lentiviral expression of IKZF1 variant 1 with Q146H mutation and FLAG tag

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1 (IKZF1 Human)

Use

Lentiviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

splice variant 1, Q146H

Promoter

CAG

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C

Plasmid

#90014

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Ago2
NBirA*-TNRC6C

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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nLuc_AP4_TEVs_P3mS

Plasmid

#119299

Purpose

Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker

Use

Luciferase

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV/3' Box_(GLuc)_TOP2

Plasmid

#68435

Purpose

Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP2 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-pEF1a-EBFP-tTAn

Plasmid

#172122

Purpose

Constitutive expression of EBFP and rtTA-InteinN

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

EBFP, tTAn-InteinN

Use

AAV

Promoter

EF1a

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_CYLD_WT

Plasmid

#82326

Purpose

Gateway Donor vector containing CYLD , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CYLD (CYLD Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-MLPmin-luc2

Plasmid

#194383

Purpose

Reporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoter

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

10xUAS-luc2

Expression

Mammalian

Promoter

MLP minimal

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-SUN1_888

Plasmid

#125851

Purpose

Expresses SUN1_888 tagged with GFP in mammalian cells

Depositor

Miki Hieda

Article

Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

Insert

SUN1_888 (SUN1 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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