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pCMU-GAr

Plasmid

#61172

Purpose

Fluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

GmMAN49-mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pSico PGK puro

Plasmid

#11586

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression.

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pJUMP24_T24_pRham-ilvA-relE

Plasmid

#201531

Purpose

Expressing synthetic overlapping gene, ilvA-relE. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Article

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE

Use

Synthetic Biology

Promoter

PrhaBAD (rhamnose)

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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TCARZ-SV40-GFP-PAmin-GF3-R4A22

Plasmid

#203769

Purpose

For production of template RNA for use in PRINT, GFP under SV40 promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for GFP

Use

Ivt template

Promoter

SV40

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMM178

Plasmid

#61270

Purpose

Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.

Depositor

Timothy Lu

Article

Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

Insert

Cas9, tracrRNA, crRNA

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Promoter

pLtetO, native, native (respectively)

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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HcKCR1_mCherry_pcDNA3.1

Plasmid

#177336

Purpose

Expresses KCR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

Insert

HcKCR1

Tags

mCherry

Expression

Mammalian

Mutation

mammalian codon-adapted

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP

Plasmid

#213119

Purpose

Expression of an ActA FKBP payload for MinDE recruitment circuits in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

ActA(1-584)-BFP-FKBP (actA Listeria monocytogenes)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-deltaBamHI-human MIZ1

Plasmid

#74167

Purpose

Mammalian expression of human MIZ1

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

MIZ1 (ZBTB17 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
NmCas9_sgRNA_expression_in_pBluescript

Plasmid

#122091

Purpose

U6 driven NmCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

NmCas9 tracrRNA (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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Stx3

Plasmid

#186685

Purpose

Expresses Syntaxin 3 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

Syntaxin 3

Tags

6 his tag

Expression

Bacterial

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3

Plasmid

#67276

Purpose

RMCE donor plasmid for FlpO. the marker gene is green fluorescent (mWasabi) with PA

Depositor

Jannik Elverløv-Jakobsen

Article

Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

Inserts

GFP (mWasabi)
hygro

Tags

F3 and Frt

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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pFW1000

Plasmid

#224210

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCMU-PDESr

Plasmid

#61206

Purpose

Fluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPDLP1

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pMJ841

Plasmid

#39318

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A) and Histidine 840 …

Promoter

T7

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pAC150-KEIMA-RAMP4

Plasmid

#201929

Purpose

PiggyBac Vector expressing KEIMA-RAMP4

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

RAMP4 (SERP1 Human)

Expression

Mammalian

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-NLuc_SV40:PuroR

Plasmid

#183045

Purpose

Expresses FLAG-tagged NanoLuciferase (NLuc-only) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

NanoLuciferase

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)::NL

Plasmid

#141289

Purpose

NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

NLUC

Use

Luciferase

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pKS diaCas9_sgRNA

Plasmid

#74923

Purpose

Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter

Depositor

Per Winge

Article

Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10

Inserts

LHCF2 promoter
diaCas9
LHCF1 terminator
U6 promoter
sgRNA
U6 3' region

Use

CRISPR

Promoter

Cas9 module and sgRNA module

Available Since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

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pTK-EdnrbE2-mCherry

Plasmid

#127772

Purpose

Enhancer reporter construct containing the chicken EdnrB enhancer E2 driving mCherry activity

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

ednrbE2

Use

Enhancer reporter construct

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pQTEV-MPP6

Plasmid

#34806

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

membrane protein, palmitoylated 6 (PALS2 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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