We narrowed to 14,066 results for: ckb
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Plasmid#177825PurposeHost Cell Reactivation (HCR) control GFP plasmid (pMax backbone)DepositorInsertGFP
ExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICH41331::pU6cm-pegR::EURb7
Plasmid#227695PurposeGolden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).DepositorInsertAltered epegRNA backbone with cloning sites for gRNA and RTT.
UseCRISPRExpressionPlantPromoterU6 compositeAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pp-CBBC-Fluc
Plasmid#232920PurposeAllows the Golden Gate (BsmBI) cloning of pegRNA between two Csy4-hairpins in the intron of a CMV-FLUC encoding plasmid (POL II): ipegRNAs must be processed by Csy4 in VLP- producer cells.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF-XMAS-1xStop
Plasmid#164411PurposeTransient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyTagsT2A-GFPExpressionMammalianPromoterEF1-alphaAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
MCS-13X Linker-BioID2-HA
Plasmid#80899PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.DepositorHas ServiceCloning Grade DNATypeEmpty backboneTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianPromoterCMVAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide_DKO
Plasmid#183193PurposePlasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screenDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterhU6, mU6Available SinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCXMG
Plasmid#204409PurposeExpression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI siteDepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromotertacAvailable SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
plenti-CMV-mCherry-T2A-GFP
Plasmid#109427PurposeA lentiviral backbone expressing mCherry-T2A-eGFP off of a CMV promoter.DepositorInsertmCherry T2A GFP
UseLentiviralPromoterCMVAvailable SinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-MCS--4x6T-WPRE
Plasmid#196417PurposeEmpty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorTypeEmpty backboneUseAAV; Astrocyte-selectiveExpressionMammalianPromoterGfaABC1DAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_mCherry_P2A_Hygro
Plasmid#135003PurposeLentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterEF1aAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB501
Plasmid#74843PurposeGateway cloning compatible binary vector for simple cloning of gene.DepositorTypeEmpty backboneExpressionPlantPromoterNo PromoterAvailable SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB401
Plasmid#74795PurposeGateway cloning compatible binary vector for simple cloning of gene.DepositorTypeEmpty backboneExpressionPlantPromoterNo PromoterAvailable SinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-multi-Guide
Plasmid#85401PurposeLentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neoDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWB441
Plasmid#74827PurposeGateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
myc-BioID2-MCS
Plasmid#74223PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneTagsMyc and humanized A. aeolicus BioID2 with R40GExpressionMammalianPromoterCMVAvailable SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB442
Plasmid#74828PurposeGateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
mycBioID2-13X Linker-MCS
Plasmid#92308PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.DepositorHas ServiceCloning Grade DNATypeEmpty backboneTagsHumanized A. aeolicus BioID2 with R40G and MycExpressionMammalianPromoterCMVAvailable SinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUltra
Plasmid#24129Purpose3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interestDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianAvailable SinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462) V2.0
Plasmid#62987PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcw107
Plasmid#62511PurposeGateway Expression VectorDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterPGKAvailable SinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only