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pIRES2-DsRed-MST-Kv2.1

Plasmid

#131707

Purpose

Expresses Kv2.1in mammalian cells

Depositor

Jennifer Kearney

Article

Jorge et al Proc Natl Acad Sci U S A. 2011 Mar 29;10

Insert

KCNB1 (KCNB1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1

Plasmid

#205443

Purpose

human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD46-HLA-G SCT-hCD47-hCD59

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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G-GECO1.2-Orai1

Plasmid

#73562

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

G-GECO1.2-Orai1 (ORAI1 Human, Synthetic)

Tags

G-GECO1.2

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_UBXN4_R3HDM1

Plasmid

#205950

Purpose

Express mEGFP-tagged fusion protein, UBXN4_R3HDM1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

UBXN4_R3HDM1 (UBXN4, R3HDM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAcBac1-EcTyr-TAG

Plasmid

#228782

Purpose

EcTyr aaRS/tRNA system for mammalian ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcTyrRS
EcYtR
BstR

Use

Baculoviral

Expression

Mammalian

Mutation

TAG suppressor

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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hKCNB1 L211P

Plasmid

#124487

Purpose

missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

Depositor

Federico Sesti

Article

Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

Insert

human KCNB1 (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed leucine 211 to proline

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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hKCNB1 G381R

Plasmid

#124490

Purpose

missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

Depositor

Federico Sesti

Article

Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

Insert

human KCNB1 (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed glycine at 381 to arginine

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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hKCNB1 F416L

Plasmid

#124491

Purpose

missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

Depositor

Federico Sesti

Article

Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

Insert

human KCNB1 (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed phenyalanine 416 to leucine

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNAI-GAL4-CREB

Plasmid

#46769

Purpose

Expression of GAL4 fusion to wild type CREB

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

GAL4(1-147)-CREB (Creb1, GAL4 Budding Yeast, Rat)

Tags

GAL4

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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TPC2(N257A)-mCherry

Plasmid

#135186

Purpose

Pore-dead mutant of TPC2, tagged on C terminus with mCherry.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC2 (Tpcn2 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Mutation of asparagine257 to alanine

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZ A-Strep-Opa1 (SB251)

Plasmid

#227600

Purpose

Inducible expression of Strep-tagged Opa1 in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Tags

Twin-StrepTag

Expression

Yeast

Mutation

contains aa 253-960 only

Promoter

AOX1

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_ATG101

Plasmid

#223796

Purpose

Expression of recombinant protein for purification (co-expression with ATG13)

Depositor

James Hurley, Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG101 (ATG101 Human)

Expression

Mammalian

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBK-EcTrpRS(H14)-EcTyrRS(VSMA,D165G)

Plasmid

#231130

Purpose

Expresses mutant E. coli TrpRSTyrRS from a glnS promoters for proteome-wide incorporation of tryptophan/tyrosine analogues.

Depositor

Abhishek Chatterjee

Article

Loynd et al Nat Chem Biol. 2025 Oct 2. doi: 10.1038/

Insert

E. coli tyrosyl tRNA synthetase

Expression

Bacterial

Mutation

EcTrpRS: S8A, V144G, V146C; EcTyrRS: Y37V, D165G,…

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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VP16-ER beta (long)

Plasmid

#11352

Depositor

Donald McDonnell

Article

Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

Insert

ER beta long (ESR2 Human)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA4c/His-Xpress-HA-hBIG1(E793K)

Plasmid

#79433

Purpose

Expresses N-terminally His6, Xpress and HA-tagged BIG1(E793K) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

Insert

BIG1 (ARFGEF1 Human)

Tags

HA, His6, and Xpress

Expression

Mammalian

Mutation

changed Glutamic acid 793 to Lysine

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only
CaV1.3e[8a,11,31b,Δ32,Δ42a]

Plasmid

#49332

Purpose

Repaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

Promoter

CMV

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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139H2_HC

Plasmid

#206201

Purpose

For recombinant expression of the full heavy chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells. Includes a C-terminal -His8 tag for purification.

Depositor

Joost Snijder

Article

Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

Insert

anti-MUC1 antibody 139H2 heavy chain (Ighg1 Mouse)

Tags

-AAAHHHHHHHH

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC

Plasmid

#114377

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targeting

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv2.1C-linker-TlcnC (NEWENTRY )

Use

AAV

Tags

EYFP and Kv2.1C-linker-TlcnC

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-p2A-full length LIC1

Plasmid

#74601

Purpose

expresses LIC1 in mammalian cells; untagged but cells indicate expression with diffuse mCherry

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 (DYNC1LI1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pb-CAGGS-dCas9-blasto

Plasmid

#218555

Purpose

Encodes CAG promoter-driven dCas9 with a C-terminal bipartite SV40 NLS, along with blasticidin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

dCas9

Use

CRISPR; Piggybac transposition

Tags

2xSV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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