161,873 results

1. pHel2

   Plasmid

   #102960

   Purpose

   E.coli/H.Pylori shuttle vector

   Depositor

   Rainer Haas

   Article

   Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

   Type

   Empty backbone

   Use

   Plasmid shuttle vector system for genetic complem…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-Anillin

   Plasmid

   #68027

   Purpose

   Full length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1

   Depositor

   Michael Glotzer

   Article

   Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

   Insert

   Anillin (ANLN Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   silent mutations at 798 5'-TGCC TCTTTGAATAAA…

   Promoter

   CMV

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-nEF-Con/Fon DREADD Gi-mCherry

   Plasmid

   #177672

   Purpose

   Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells

   Depositor

   Karl Deisseroth

   Has Service

   AAV8

   Insert

   DREADD Gi-mCherry
   

   Use

   AAV, Cre/Lox, and Mouse Targeting ; Intrsect

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   nEF

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-GRAB_OT1.0

   Plasmid

   #185386

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Has Service

   AAV1 and AAV9

   Insert

   GPCR activation based oxytocin sensor GRAB_OT1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-3xFLAG-GFP-puroR

   Plasmid

   #167463

   Purpose

   3rd-gen lentiviral expression of FLAG-GFP in mammalian cells

   Depositor

   Steven Artandi

   Article

   Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. RFP-HA-DAT

   Plasmid

   #90265

   Purpose

   Expresses RFP-HA-DAT in mammalian cells

   Depositor

   Alexander Sorkin

   Article

   Caltagarone et al Mol Cell Neurosci. 2015 Sep;68:120-30. d

   Insert

   DAT (dopamine transporter) (SLC6A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   inserted HA tag (see YFP-HA-DAT)

   Promoter

   CMV

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-FLEX-EGFP-KASH

   Plasmid

   #154373

   Purpose

   Vector for Cre-dependent expression of EGFP tagged KASH protein domain

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   EGFP-KASH
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. V5-APEX2-SENP2

   Plasmid

   #129276

   Purpose

   Expression of APEX2 at nuclear pore

   Depositor

   Alice Ting

   Article

   Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

   Insert

   V5-APEX2-SENP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a_FLI1_P2A_Hygro_Barcode

   Plasmid

   #120437

   Purpose

   Barcoded lentiviral vector to express FLI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   FLI1 (FLI1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. LRT2B-GO2

    Plasmid

    #136909

    Purpose

    Lentivirus expressing GO2 sgRNA

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    sgGO2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    hU6

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60227

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBUN6A11

    Plasmid

    #50579

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-VP64
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Expression

    Mutation

    dCas9 = defective in DNA cleavage; 4×minimal VP16…

    Promoter

    OsU3p and Ubi1p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. aCKAR

    Plasmid

    #124427

    Purpose

    FRET-based reporter for monitoring aPKC activity in cells

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al Sci Signal. 2019 Jan 1;12(562). pii: 12/

    Insert

    aCKAR
    

    Use

    Tags

    mCFP and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-HA-H-Ras_wt

    Plasmid

    #39503

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS (HRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-Sequestosome1-N-18

    Plasmid

    #55132

    Purpose

    Localization: Membrane Scaffold, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Sequestosome1 (SQSTM1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pBabe 12S E1A

    Plasmid

    #18742

    Depositor

    Scott Lowe

    Article

    Samuelson et al Proc Natl Acad Sci U S A. 1997 Oct 28. 9

    Insert

    12S E1A
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pS23·Pm-GFP

    Plasmid

    #122593

    Purpose

    Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    Pm promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-N-CretrcintG

    Plasmid

    #69570

    Purpose

    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

    Has Service

    AAV1

    Insert

    kozak consensus - N-CretrcintG
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDZ513 (pHIS MET25pro PP7-PS-yeGFP)

    Plasmid

    #45931

    Depositor

    Daniel Zenklusen

    Article

    Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

    Insert

    PCP-PS
    

    Use

    Tags

    2x-yeGFP

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. WFS1-Flag-pcDNA3

    Plasmid

    #13011

    Depositor

    Fumihiko Urano

    Article

    Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

    Insert

    Wolfram Syndrome 1 (WFS1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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