159,481 results

1. pTECH-MmAcK3RS(IPYE)

   Plasmid

   #104071

   Purpose

   Expression plasmid encoding MmAcK3RS(IPYE) and pylT

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp MmAcK3RS(IPYE); PproK pyl
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSICO-eGFP-TNPO3

   Plasmid

   #167590

   Purpose

   Expresses human TNPO3 fused to eGFP

   Depositor

   Zandrea Ambrose

   Article

   Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

   Insert

   eGFP-TNPO3 (TNPO3 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mutation

   WT

   Promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2

   Plasmid

   #112004

   Purpose

   For enriched expression of GCaMP6s in axons with cytosolic expression of mKate2

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Has Service

   AAV9

   Insert

   axon-GCaMP6s-P2A-mKate2
   

   Use

   AAV

   Tags

   GAP43 palmitoylation domain

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. GUT BuGZ FL

   Plasmid

   #84024

   Purpose

   Expression of GFP tagged BuGZ in human cells

   Depositor

   Patrick Paddison

   Article

   Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

   Insert

   BuGZ (ZNF207 Human)
   

   Use

   Lentiviral

   Tags

   TurboGFP

   Expression

   Mammalian

   Mutation

   Q394R

   Promoter

   UCOE / SFFV

   Available since

   Oct. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. DRD2-Tango

   Plasmid

   #66269

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   DRD2 (DRD2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDestTol2-QUAS:GFP-CAAX

   Plasmid

   #184813

   Purpose

   Used to generate the Tg(QUAS:GFP-CAAX)c591 transgenic line

   Depositor

   Marnie Halpern

   Article

   Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

   Insert

   QUAS:GFP-CAAX-pA
   

   Use

   Zebrafish tol2 transgenesis

   Tags

   GFP-CAAX

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

   Plasmid

   #210525

   Purpose

   AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   mtagBFP-KASH
   U6-gRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBEVY-GL

   Plasmid

   #51225

   Purpose

   bi-directional expression vector for yeast, galactose inducible, LEU2 selection

   Depositor

   Charles Miller

   Article

   Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1,10

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Vamp2-pHluorin

   Plasmid

   #190151

   Purpose

   Expresses (pH-sensitive) super ecliptic pHluorin-tagged mouse/rat Vamp2 for imaging exocytosis

   Depositor

   Brad Zuchero

   Article

   Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

   Insert

   Vamp2 (Vamp2 Mouse)
   

   Use

   AAV

   Tags

   superecliptic pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SZ_EC_02

    Plasmid

    #155341

    Purpose

    Overexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XR

    Depositor

    Jens Nielsen

    Article

    Konzock et al BMC Microbiol. 2021 Mar 8;21(1):77. doi:

    Inserts

    tef1 promoter - xylulose kinase - pex20 terminator
    pyk1 promoter - xylose reductase - pex16 terminator
    gapdh promoter - xylitol dehydrogenase - lip2 terminator
    

    Use

    Easycloneyali insert vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    gapdh, pyk1, and tef1

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. ARTX_ctrl

    Plasmid

    #214065

    Purpose

    Fluorescent reporter system, NMD negative control. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    TurboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Remnant of linker sequence present at the end of …

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PTPRC-bio-His

    Plasmid

    #51813

    Purpose

    Expresses full-length Leukocyte common antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PTPRC (PTPRC Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15-ZmPYK

    Plasmid

    #220230

    Purpose

    Inducible expression of Zymomonas mobilis pyruvate kinase

    Depositor

    Liskin Swint-Kruse, Aron Fenton

    Article

    Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

    Insert

    Zymomonas mobilis pyruvate kinase (pyk, ZMO0152 Zymomonas mobilis)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEZYmyc-His

    Plasmid

    #18701

    Purpose

    pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

    Plasmid

    #159908

    Purpose

    Mutagenesis of Htr2a

    Depositor

    Larry Zweifel

    Insert

    Htr2a (Htr2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVSV eGFP RABV-G

    Plasmid

    #31833

    Depositor

    Connie Cepko

    Article

    Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    VSV genome
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSG5 Large T

    Plasmid

    #9053

    Depositor

    William Hahn

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-APEX2-C Cloning Vector

    Plasmid

    #128146

    Purpose

    To express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEM

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Flag-APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF CAG luc puro

    Plasmid

    #67501

    Purpose

    Constitutive expression of firefly luciferase in mammalian cells

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRX-IZ-ANXA6-mRuby3

    Plasmid

    #184892

    Purpose

    Stable expression of ANXA6-mRuby3 (Annexin A6-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA6 (ANXA6 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    R587K

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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