We narrowed to 12,182 results for: pcDNA

Showing: 11161 - 11180 of 12182 results

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AfrLEA3m_mCh-2xFKBP (pBS1137)

Plasmid

#185321

Purpose

For the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfrLEA3m

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-del.GW-I+II+III-NES-mut

Plasmid

#42040

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

deleted aa457-532, aa716-772 and aa804-924, F951A…

Promoter

CMV

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pRD398

Plasmid

#163105

Purpose

Expression of mCherry_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_mCherry_H-NSdbd_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pTOPO-MCL1S159A

Plasmid

#21606

Depositor

Ulrich Maurer

Article

Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

Insert

MCL-1 S159A (MCL1 Human)

Expression

Mammalian

Mutation

Serine 159 mutated to Alanine, abolishing phosph…

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pc-FLAGCSK-SH3-SH2 (2 to 190 amino acids)

Plasmid

#74505

Purpose

Overexpression of CSK SH3-SH2 in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

Insert

CSK (CSK Human)

Tags

FLAG

Expression

Mammalian

Mutation

SH3-SH2 domains

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
BirA-Myc_N_HNF1a

Plasmid

#167710

Purpose

Expresses HNF1a in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

HNF1a (HNF1A Human)

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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Opto-GPR150

Plasmid

#106050

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR150; Opto-GPR150; E4)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR150 (GPR150 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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HA-HIF1a (S668A)

Plasmid

#49463

Purpose

HA-tagged HIF-1a Ser668 resuide mutated to Ala

Depositor

Wafik El-Deiry

Article

Warfel et al Cell Cycle. 2013 Oct 25;12(23).

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 668 to Alanine

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-MED9

Plasmid

#15407

Depositor

Joan Conaway, Ronald Conaway

Article

Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

Insert

MED9 (Med9 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pRD190

Plasmid

#163100

Purpose

Expression of mEos3.2 in eukaryotes to stain the nuclear compartment

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_mEos3.2_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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CAHS2parrc-mCh-2xFKBP (pBS1109)

Plasmid

#185304

Purpose

For the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2parrc

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-FLAG

Plasmid

#105783

Purpose

Expression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-FLAG

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTE3987

Plasmid

#137716

Purpose

Expresses dLbCas12a-RVRR-BE in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

Insert

dLbCas12a-RVRR-BE

Tags

6xHis, APOBEC-1, SV40 NLS, and UGI

Expression

Mammalian

Mutation

G532A, K538V, Y542R, K595R

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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hMEF2A K403R

Plasmid

#118355

Purpose

This plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hMEF2A (MEF2A Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K403R

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-NLS/NES-mut

Plasmid

#42002

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

R930A, R931A, R932A, R934A, F951A, F955A, I958A, …

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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pIKAT111

Plasmid

#69996

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

C1459T (R487C)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRR.6

Plasmid

#69997

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

Q172H, K262R

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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FR_HNF1B_A263insGG

Plasmid

#31103

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF1B (HNF1B Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

mutation A263insGG

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2 C548A

Plasmid

#126592

Purpose

Expresses siRNA resistant SENP2 (catalytic dead) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C548A

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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C211

Plasmid

#174360

Purpose

For expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfection

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (PABPC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

I110L, D111E, D117E, A121G, G139A, Y140F, T147S, …

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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