174,397 results

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pEF6-myc-TBC1D15

Plasmid

#79148

Purpose

mammalian expression of TBC1D15

Depositor

Aimee Edinger

Article

Peralta et al J Biol Chem. 2010 May 28. 285(22):16814-

Insert

TBC1D15 (Tbc1d15 Mouse)

Tags

His, Myc, and V5

Expression

Mammalian

Mutation

S616N (please see depositor comment below)

Promoter

EF-1alpha

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP gRNA (BRDN0000561167)

Plasmid

#80034

Purpose

3rd generation lentiviral gRNA plasmid targeting EGFP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-AID-EGFP

Plasmid

#80076

Purpose

Mammalian expression vector for C-terminal AID-EGFP tagging

Depositor

Andrew Holland

Insert

AID-EGFP

Tags

AID and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab11a

Plasmid

#79805

Purpose

Express TagBFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab11a (RAB11A Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pGWB517

Plasmid

#74859

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-V2-ORF

Plasmid

#73636

Purpose

Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pNICKclos1.0

Plasmid

#73639

Purpose

Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

Cas9 nickase
sGRNA to pyrE

Use

E.coli-clostridium shuttle vector

Mutation

D10A

Promoter

j23119 and ptb

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pUCP20T-eyfp

Plasmid

#78466

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Article

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

eYFP

Expression

Bacterial

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGAMA-empty

Plasmid

#74755

Purpose

Mammalian lentiviral vector

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-DIO-Magneto2.0-sNRPpA

Plasmid

#74307

Purpose

Cre-dependent expression of Magneto2.0 in mammalian cells

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

Magneto2.0 (FTL, FTH1, Trpv4 Rat, Human)

Use

AAV and Cre/Lox

Tags

Flag

Expression

Mammalian

Mutation

TRPV4: delta 760-871

Promoter

CMV (after Cre recombination)

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCR3.1-mIL2

Plasmid

#74467

Purpose

expression of mouse IL2 in mammalian cells

Depositor

Loree Heller

Article

Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

Insert

IL2 (Il2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-SLC38A9.1

Plasmid

#71855

Purpose

overexpression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGE011

Plasmid

#71537

Purpose

For CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pQE30-hPolB

Plasmid

#70761

Purpose

bacterial expression of human DNA Polymerase beta (POLB)

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

POLB (POLB Human)

Tags

6x His

Expression

Bacterial

Mutation

R333L

Promoter

T5/lac operon

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMCSG7-Wt-NmeCas9

Plasmid

#71474

Purpose

bacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-WT-NmeCas9

Tags

6His

Expression

Bacterial

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1_SEPT9 i1

Plasmid

#71622

Purpose

mammalian expression of human SEPT9 with an mCherry fusion

Depositor

Elias Spiliotis

Article

Dolat et al J Cell Biol. 2014 Oct 27;207(2):225-35.

Insert

Septin 9 (SEPTIN9 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCAM-kan-R4R3

Plasmid

#71275

Purpose

Binary destination vector for plant transformation. Kanamycin resistance.

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Type

Empty backbone

Use

Gateway Cloning

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO_FOXA1_#1

Plasmid

#70095

Purpose

Expression of shRNA to human FOXA1, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pGZ108 (pFA6a-3FLAG-MNase-kanMX6)

Plasmid

#70231

Purpose

ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

Depositor

Steven Henikoff

Article

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

3xFLAG tag and aa 83-231 of Mnase

Use

Yeast genomic targeting

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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CAG-FLEx(FRT)-G

Plasmid

#67828

Purpose

Flp-dependent expression of rabies glycoprotein to mediate trans-synaptic spread of modifed rabies virus in cells

Depositor

Liqun Luo

Article

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

rabies glycoprotein

Use

AAV

Expression

Mammalian

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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