We narrowed to 161,916 results for: addgene

1. B2M-SDMutation-gRNA

   Plasmid

   #215547

   Purpose

   gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   B2M (B2M Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCriprV2-sgRNA-PER1-#2

   Plasmid

   #189988

   Purpose

   Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982

   Depositor

   Achim Kramer

   Article

   Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

   Insert

   Period1 (PER1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mito-PDCD10-mScarlet-I

   Plasmid

   #208887

   Purpose

   Express mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   CCM3 (PDCD10 Human)
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   PDCD10 targeted to the mitonchondria

   Promoter

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUGW-shRIIα-RIIαS97A-IRES-EGFP

   Plasmid

   #183457

   Purpose

   Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA and Prkar2a (Prkar2a Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   shRNA-resistant mutations: C135>G, G138>A, …

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUGW-shRIIα-RIIαS97E-IRES-EGFP

   Plasmid

   #183458

   Purpose

   Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA and Prkar2a (Prkar2a Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   shRNA-resistant mutations: C135>G, G138>A, …

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 564aa ELL2 bluescript

   Plasmid

   #127267

   Purpose

   For in vitro translation of human ELL2 lacking COOH region

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   Addgene Plasmid 127256--SphI site filled in, then…

   Promoter

   T7

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

   Plasmid

   #58490

   Purpose

   Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3

   Insert

   Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Aspartic Acid 95 to Glutamine

   Promoter

   Ubiquitin

   Available since

   Aug. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX304_CdTag-HA_EmptyVector

   Plasmid

   #221466

   Purpose

   Gateway backbone for C-terminal dTag cloning

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FKBP F36V

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO-Tet-On-ARNTL-shRNA1

   Plasmid

   #166913

   Purpose

   Lentivirus for inducible knockdown of human ARNTL(BMAL1)

   Depositor

   Roland Friedel

   Insert

   ARNTL shRNA (BMAL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX311_CdTag-HA_EmptyVector

    Plasmid

    #221468

    Purpose

    Gateway backbone for C-terminal dTag cloning

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FKBP F36V

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRE112

    Plasmid

    #43828

    Depositor

    Dieter Schifferli

    Article

    Edwards et al Gene. 1998 Jan 30;207(2):149-57.

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb1

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid

    #118154

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    EF1a core and U6

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMV-FLEX-SaCas9-U6-sgRosa26

    Plasmid

    #159914

    Purpose

    Mutagenesis of Rosa26 with SauCas9

    Depositor

    Larry Zweifel

    Insert

    Rosa26 gRNA (Gt(ROSA)26Sor Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCM66

    Plasmid

    #45827

    Depositor

    Mary Lidstrom

    Article

    Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75

    Type

    Empty backbone

    Use

    Broad host range cloning vector in bacteria

    Tags

    Expression

    Mutation

    Promoter

    lac

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. 5E26 (PANK2)

    Plasmid

    #79516

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PANK2 (PANK2 Human)
    

    Use

    Baculovirus expression

    Tags

    6xHis and TEV cleavage site

    Expression

    Mutation

    Promoter

    polyhedrin

    Available since

    June 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. 7TGC

    Plasmid

    #24304

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-eGFP // SV40-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    EGFP is GFP with S65T and F64L mutations

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUK21

    Plasmid

    #49788

    Purpose

    E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

    Depositor

    Joachim Messing

    Article

    Vieira et al Gene. 1991 Apr;100:189-94.

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lacZ

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDMS197

    Plasmid

    #43831

    Depositor

    Dieter Schifferli

    Article

    Edwards et al Gene. 1998 Jan 30;207(2):149-57.

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb1

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRE118

    Plasmid

    #43830

    Depositor

    Dieter Schifferli

    Article

    Edwards et al Gene. 1998 Jan 30;207(2):149-57.

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb1

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIRE YIC

    Plasmid

    #18784

    Depositor

    Han Htun

    Article

    Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

    Insert

    H-ferritin iron response element
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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