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pET30ax

Plasmid

#85761

Purpose

A bacterial expression vector with a His-tag at N and C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Use

Tags

His tag (N-term and C-term)

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pUdO1a

Plasmid

#210226

Purpose

high-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTagRFP657-C1

Plasmid

#31872

Purpose

For fusion of TagRFP657 to N-term of insert. SEE DEPOSITOR COMMENTS BELOW.

Depositor

Vladislav Verkhusha

Article

Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

Insert

TagRFP657

Use

Tags

Expression

Mammalian

Mutation

M44Q, K69H, F84W, S148H, S165T, D166A, M167L, L18…

Promoter

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pBABE-puro SHP2 WT

Plasmid

#8329

Depositor

Ben Neel

Article

Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

Insert

SHP2 (PTPN11 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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pLenti-FRA1

Plasmid

#188665

Purpose

Expresses murine FRA1 (Fosl1)

Depositor

Günter Schneider

Article

Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

Insert

Fosl1 (Fosl1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-10V5

Plasmid

#181938

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xV5

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAG425GAL-ccdB-EGFP

Plasmid

#14201

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

Plasmid

#131004

Purpose

bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

Kv2.1-HA

Expression

Mutation

Promoter

hSyn

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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APOE_Halo_C_allele

Plasmid

#178121

Purpose

Donor vector for endogenous tagging of human APOE at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (APOE Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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HA-MAP1LC3C

Plasmid

#137758

Purpose

Expression of LC3C

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

MAP1LC3C (MAP1LC3C Human)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - EGFP sgRNA 1

Plasmid

#51760

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Article

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS and U6

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-CLC

Plasmid

#193559

Purpose

Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-EGFP-NLS

Plasmid

#165441

Purpose

Expresses nuclear EGFP from CMV promoter

Depositor

Esteban Engel

Article

Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

Insert

EGFP-NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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PSMA4_GFP_Cterm

Plasmid

#221514

Purpose

Fluorescently tagged Psma4-GFP

Depositor

Alessandro Ori

Insert

PSMA4 (PSMA4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b-CPDBamHI-Leu

Plasmid

#38256

Depositor

Aimee Shen, Matthew Bogyo

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Use

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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mCherry-Lrrc59

Plasmid

#226565

Purpose

Encodes Lrrc59 protein laebeled with mCherry

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

Lrrc59 (Leucine-rich repeat containing protein 59) (LRRC59 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1484 - pAAV SYN1 HA-hM4D(Gi)

Plasmid

#121538

Purpose

An adeno-associated viral vector expressing HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter

Depositor

Christopher Richie

Article

Galvan et al Eur J Neurosci. 2019 May 7. doi: 10.1111

Has Service

AAV2 and AAV5

Insert

HA-tagged hM4D(Gi) (CHRM4 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

SYN1

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pP450cam

Plasmid

#85085

Purpose

Expresses P450cam (CYP101A1) in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

P450cam

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pSM-GFPnovo2

Plasmid

#116943

Purpose

GFPnovo2_unc-54 utr vector for expression in C. elegans

Depositor

Kota Mizumoto

Article

Hendi et al MicroPubl Biol. 2018 Sep 6;2018:10.17912

Insert

C. elegans codon optimized GFPnovo2

Use

Tags

GFPnovo2

Expression

Worm

Mutation

Y145F, V163A, S202T, L221V

Promoter

none

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Tbx20

Plasmid

#72697

Purpose

Doxycyline regulated expression of Tbx20 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Tbx20 (Tbx20 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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