Addgene

We narrowed to 60,826 results for: cell;

Showing: 2201 - 2240 of 60826 results

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p6VP1

Plasmid

#24725

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

VP1 from Human Polyomavirus 6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
p7VP1

Plasmid

#24726

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

VP1 from Human Polyomavirus 7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pQCXI Neo DsRed-LC3-GFP

Plasmid

#31183

Depositor

David Sabatini

Article

Sheen et al Cancer Cell. 2011 May 17. 19(5):613-28.

Insert

DsRed-LC3-GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-UBF

Plasmid

#26672

Depositor

Sui Huang

Article

Chen et al J Cell Biol. 2001 Apr 2. 153(1):169-76.

Insert

UBF1 (UBTF Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only
LRG2.1-mOrange

Plasmid

#124772

Purpose

3rd generation lentiviral plasmid to co-express a guide RNA and mOrange

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGTetRnls

Plasmid

#26599

Depositor

Peter Andrews

Article

Zafarana et al Stem Cells. 2009 Apr . 27(4):776-82.

Insert

Tetracycline repressor (TetR E.Coli)

Use

Tags

nls

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pHR Fyn-TagBFP

Plasmid

#171035

Purpose

TagBFP-tagged Fyn kinase

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Fyn

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GG-dest

Plasmid

#69538

Purpose

Destination plasmid for gRNA concatenation Golden Gate cloning

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR Lck-TagBFP

Plasmid

#171034

Purpose

TagBFP-tagged Lck kinase

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Lck

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
7TC

Plasmid

#24315

Depositor

Roel Nusse

Article

ten Berge et al Cell Stem Cell. 2008 Nov 6. 3(5):508-18.

Insert

7xTcf-mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB768

Plasmid

#60139

Purpose

Green click beetle luciferase from Promega driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBG99

Use

Tags

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-miR17

Plasmid

#21113

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 9. ()

Insert

miR-17 (MIR17 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
p273 EF1a_mCherry2_hH11

Plasmid

#164852

Purpose

mCherry2-expression vector for human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

mCherry2

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiGuide-BC-start

Plasmid

#127168

Purpose

Entry plasmid for two-step guide-barcode cloning

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

N/A

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE80L SpyTag-ELP-SpyTag

Plasmid

#112634

Purpose

Two SpyTags linked by an elastin-like polypeptide for hydrogel formation

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

SpyTag-ELP-SpyTag

Use

Tags

His6

Expression

Bacterial

Mutation

central cysteine

Promoter

T5

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-miR30

Plasmid

#20670

Depositor

Bryan Cullen

Article

Zeng et al Mol Cell. 2002 Jun . 9(6):1327-33.

Insert

miR-30 (MIR30A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
iDuet101a

Plasmid

#17629

Depositor

Linzhao Cheng

Article

Zhou et al Stem Cells. 2007 Mar . 25(3):779-89.

Insert

TetO7 - EF-1a promoter - GFP - PGK promoter - Hygro

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB1609_pRR-EGFP

Plasmid

#65852

Purpose

EGFP-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

Depositor

Marc Bühler

Article

Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

Insert

split EGFP coding sequence

Use

CRISPR and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-Rex1

Plasmid

#13368

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Zinc finger protein 42 (Zfp42 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pMXs-Dppa3

Plasmid

#13365

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

developmental pluripotency associated 3 (Dppa3 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pCIS-krPAM1

Plasmid

#25456

Depositor

Richard Mains

Article

Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

Insert

PAM1 (Pam Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pAd NeuroD-I-nGFP

Plasmid

#19414

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Aug 27. ():.

Insert

neurogenic differentiation 1 (Neurod1 Mouse)

Use

Adenoviral; Entry vector

Tags

GFP and IRES

Expression

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
hsp70:mKate2-NWASP-CA

Plasmid

#105967

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

NWASP-CA

Use

Tags

mKate2

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCVII-BFP

Plasmid

#162755

Purpose

Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

mTagBFP2

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
3494 pcDNA3 Bax

Plasmid

#8779

Depositor

Stanley Korsmeyer

Article

Oltvai et al Cell 1993 Aug 27;74(4):609-19.

Insert

Bax (Bax Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pZH516

Plasmid

#102669

Purpose

Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG86-J23151-LasR

Plasmid

#102456

Purpose

Expresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

LasR (lasR )

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23151

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd 2B Ngn3-I-nGFP

Plasmid

#19410

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Aug 27. ():.

Insert

neurogenin 3 (Neurog3 Mouse)

Use

Adenoviral; Entry vector

Tags

GFP and IRES

Expression

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
pZH514

Plasmid

#102667

Purpose

Constitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG84-pLas-TetRssrA

Plasmid

#102455

Purpose

Expresses TetR protein with ssrA degradation tag. The expression is controlled by a pLas promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

TetR (tetR )

Use

Tags

ssrA

Expression

Bacterial

Mutation

Promoter

pLas

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB778

Plasmid

#60154

Purpose

Green click beetle luciferase (Promega) driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBG99

Use

Tags

Expression

Yeast

Mutation

Promoter

LEU1pr

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pPolII-Luc

Plasmid

#17223

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

PolII promoter

Use

Luciferase

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pk-myc-Par6A

Plasmid

#15472

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Insert

Partitioning-defective protein 6A (Pard6g Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pRK5myc Rac1 N17

Plasmid

#15904

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Rac1 (RAC1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

T17N

Promoter

Available since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pQE80L TriCatcher-ELP

Plasmid

#112630

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-ELP

Use

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T5

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pZH520

Plasmid

#102673

Purpose

Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pZH521

Plasmid

#102674

Purpose

Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG13-pBAD-TetRssrA

Plasmid

#102449

Purpose

Expresses TetR protein with ssrA degradation tag. The expression is controlled by a pBAD promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

TetR (tetR )

Use

Tags

ssrA

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB648

Plasmid

#60140

Purpose

NanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Use

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
iDuet011a

Plasmid

#17628

Depositor

Linzhao Cheng

Article

Zhou et al Stem Cells. 2007 Mar . 25(3):779-89.

Insert

TetO7 - UBC promoter - GFP - PGK promoter - Hygro

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Showing: 2201 - 2240 of 60826 results

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